Specific point mutations in key redox enzymes are associated with chemoresistance in epithelial ovarian cancer

Fletcher, N.M.; Belotte, J; Saed, Mohammed G.; Memaj, Ira; Diamond, Michael P.; Morris, Robert T.; Saed, Ghassan M.

doi:10.1016/j.freeradbiomed.2016.11.028

Cited by 36 publications

(66 citation statements)

References 61 publications

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“…The correction or deletion of resistance‐related genes is a promising development for tumor therapy. Current studies on drug resistance of tumor cells using CRISPR/Cas9 in lung cancer, breast cancer, melanoma, urogenital tumors, and gastrointestinal tumors about chemotherapy drugs, targeted drugs, and inhibitors were summarized in Table . Besides the above‐mentioned studies, there are other studies on the mechanisms related to drug resistance of other malignant tumors cells by using CRISPR/Cas9.…”

Section: Using Crispr/cas9 In the Study Of Tumor Therapiesmentioning

confidence: 99%

“…Current studies on drug resistance of tumor cells using CRISPR/Cas9 in lung cancer, breast cancer, melanoma, urogenital tumors, and gastrointestinal tumors about chemotherapy drugs, targeted drugs, and inhibitors were summarized in Table 2. 56,88,94,97,100,113,[141][142][143][144][145][146][147][148][149][150][151][152][153][154][155] Compared with wild-type cells, ATG5 KO cells had stronger migration ability and significantly increased resistance to the Src tyrosine kinase inhibitor PP2. 156 The results showed that autophagy deficiency can induce malignant cell transformation and resistance to PP2.…”

Section: Drug Resistance Study Of Tumorsmentioning

confidence: 99%

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Application of CRISPR/Cas9 gene editing technique in the study of cancer treatment

et al. 2019

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In recent years, gene editing, especially that using clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9, has made great progress in the field of gene function. Rapid development of gene editing techniques has contributed to their significance in the field of medicine. Because the CRISPR/Cas9 gene editing tool is not only powerful but also has features such as strong specificity and high efficiency, itcan accurately and rapidly screen the whole genome, facilitating the administration of gene therapy for specific diseases. In the field of tumor research, CRISPR/Cas9 can be used to edit genomes to explore the mechanisms of tumor occurrence, development, and metastasis. In these years, this system has been increasingly applied in tumor treatment research. CRISPR/Cas9 can be used to treat tumors by repairing mutations or knocking out specific genes. To date, numerous preliminary studies have been conducted on tumor treatment in related fields. CRISPR/Cas9 holds great promise for gene-level tumor treatment. Personalized and targeted therapy based on CRISPR/Cas9 will possibly shape the development of tumor therapy in the future. In this study, we review the findings of CRISPR/Cas9 for tumor treatment research to provide references for related future studies on the pathogenesis and clinical treatment of tumors.

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Section: Using Crispr/cas9 In the Study Of Tumor Therapiesmentioning

confidence: 99%

Section: Drug Resistance Study Of Tumorsmentioning

confidence: 99%

Application of CRISPR/Cas9 gene editing technique in the study of cancer treatment

et al. 2019

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“…Pro-oxidant enzymes such as myeloperoxidase (MPO), inducible nitric oxide synthase (iNOS) and NAD(P)H oxidase have been associated with initiation, progression, survival, and increased risk in cancers such as breast, ovarian, lung, prostate, bladder, colorectal and malignant melanoma [21,44]. Moreover, the expression of those key pro-oxidant enzymes was found to change based on the histological type and grade of the tumor [21,45,46]. Likewise, antioxidants have also been associated with initiation, progression, survival, and increased risk in cancers such as lung, head and neck, and prostate cancer [47][48][49][50].…”

Section: Cancer Cells Are Under Intrinsic Oxidative Stressmentioning

confidence: 99%

“…Likewise, antioxidants have also been associated with initiation, progression, survival, and increased risk in cancers such as lung, head and neck, and prostate cancer [47][48][49][50]. The expression of GSR and GPX, key antioxidant enzymes, has also been reported to be altered in various types of cancer [21]. The activity and expression of SOD, a powerful antioxidant enzyme, has been reported to be decreased in colorectal carcinomas, pancreatic, lung, gastric, ovarian, and breast cancers [21,45,46].…”

Section: Cancer Cells Are Under Intrinsic Oxidative Stressmentioning

confidence: 99%

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New Insights into the Pathogenesis of Ovarian Cancer: Oxidative Stress

Saed¹,

Morris²,

Fletcher³

2018

Ovarian Cancer - From Pathogenesis to Treatment

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Ovarian cancer is the leading cause of death from gynecologic malignancies yet the underlying pathophysiology is not clearly established. Epithelial ovarian cancer (EOC) has long been considered a heterogeneous disease with respect to histopathology, molecular biology, and clinical outcome. Treatment of ovarian cancer includes a combination of cytoreductive surgery and combination chemotherapy, with platinums and taxanes. Despite initial success, over 75% of patients with advanced disease will relapse around 18 months and the overall 5-year survival is approximately 50%. Cancer cells are known to be under intrinsic oxidative stress, which alters their metabolic activity and reduces apoptosis. Epithelial ovarian cancer has been shown to manifest a persistent pro-oxidant state as evident by the upregulation of several key oxidant enzymes in EOC tissues and cells. In the light of our scientific research and the most recent experimental and clinical observations, this chapter provides the reader with up to date most relevant findings on the role of oxidative stress in the pathogenesis and prognosis of ovarian cancer, as well as a novel mechanism of apoptosis/survival in EOC cells.

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The Current Landscape of 3D In Vitro Tumor Models: What Cancer Hallmarks Are Accessible for Drug Discovery?

Rodenhizer

Dean

D’Arcangelo

et al. 2018

Adv Healthcare Materials

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Cancer prognosis remains a lottery dependent on cancer type, disease stage at diagnosis, and personal genetics. While investment in research is at an all-time high, new drugs are more likely to fail in clinical trials today than in the 1970s. In this review, a summary of current survival statistics in North America is provided, followed by an overview of the modern drug discovery process, classes of models used throughout different stages, and challenges associated with drug development efficiency are highlighted. Then, an overview of the cancer hallmarks that drive clinical progression is provided, and the range of available clinical therapies within the context of these hallmarks is categorized. Specifically, it is found that historically, the development of therapies is limited to a subset of possible targets. This provides evidence for the opportunities offered by novel disease-relevant in vitro models that enable identification of novel targets that facilitate interactions between the tumor cells and their surrounding microenvironment. Next, an overview of the models currently reported in literature is provided, and the cancer biology they have been used to explore is highlighted. Finally, four priority areas are suggested for the field to accelerate adoption of in vitro tumour models for cancer drug discovery.

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Specific point mutations in key redox enzymes are associated with chemoresistance in epithelial ovarian cancer

Cited by 36 publications

References 61 publications

Application of CRISPR/Cas9 gene editing technique in the study of cancer treatment

Application of CRISPR/Cas9 gene editing technique in the study of cancer treatment

New Insights into the Pathogenesis of Ovarian Cancer: Oxidative Stress

The Current Landscape of 3D In Vitro Tumor Models: What Cancer Hallmarks Are Accessible for Drug Discovery?

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