1981
DOI: 10.1073/pnas.78.1.120
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Specific phospholipids are required to reconstitute adenylate cyclase solubilized from rat brain.

Abstract: (5,6). Examples exist where this modulation seems to reflect changes in membrane fluidity (7,8) and others where there appears to be a requirement for certain specific phospholipids for enzyme activity (9-11). Such investigations have been extended to the study of adenylate cyclase principally by the direct addition of phospholipids' (or cholesterol) to cells or membranes by lipic4 fusion or exchange or by supplemention of the medium of auxotrophic mutant cells with phospholipid precursors (12)(13)(14)(15). Al… Show more

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Cited by 47 publications
(12 citation statements)
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“…(37). Solubilized adenylate cyclase was reported to require phosphatidylcholine or sphingomyelin, whereas phosphatidylserine was without effect (38). A similar pattern was observed for UDP-glucuronosyltransferase from guinea pig liver (39).…”
Section: Methodsmentioning
confidence: 51%
“…(37). Solubilized adenylate cyclase was reported to require phosphatidylcholine or sphingomyelin, whereas phosphatidylserine was without effect (38). A similar pattern was observed for UDP-glucuronosyltransferase from guinea pig liver (39).…”
Section: Methodsmentioning
confidence: 51%
“…Reconstitution of Guanylate Cyclase into Phosphatidylcholine Vesicles-Guanylate cyclase was reconstituted into membrane vesicles using a dilution method similar to that of Hebdon et al (24). Phosphatidylcholine, NaCl, MgCl 2 , and Hepes, pH 7.6, were added to a concentrated fraction of purified guanylate cyclase in 2.5 mM DDM, 0.025% phosphatidylcholine, 20% glycerol, 120 mM NaCl, 6 mM EGTA, 10 mM Hepes, pH 7.6, to make a final concentration of 5 mg/ml phosphatidylcholine, 120 mM NaCl, 5 mM MgCl 2 , and 10 mM Hepes.…”
Section: Materials-8-n 3 [␣-mentioning
confidence: 99%
“…True, but it must be recalled that (i) detergents, in contrast to phospholipids, uncouple the functional units of adenylate cyclase (38,45), whereas phospholipids augment their interaction (39,40). Thus, if we find that both Ns and C are associated in detergent, in all likelihood they are associated even tighter within the lipid bilayer, and (ii) the shuttle model is based on experiments performed in digitonin extracts in the absence of added phospholipids to stabilize the components (12,44).…”
Section: C8ec9mentioning
confidence: 99%
“…This value is similar to the size of S49 lymphoma adenylate cyclase (38 NsC, however, is less stable than N' p[NH]ppG-C' on DEAE-Sephacel, probably because active N, confers more stability on C than native inactive Ns. The presence of phospholipids during the purification probably augments the Nsto-C interaction (39,40). To resolve Ns from C, the combination of detergent with high salt is required (15,41).…”
Section: C8ec9mentioning
confidence: 99%