Specific PCR Detection of Four Quarantine Fusarium Species in Korea

Hong, Sae Yeon; Kang, Moon Gi; Choi, Eun Ji; Kim, Hee Kyoung; Yun, Sung Hwan

doi:10.5423/ppj.2010.26.4.409

Cited by 21 publications

(11 citation statements)

References 13 publications

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“…incarnatum , also known by the synonyms F. pallidoroseum and F. semitectum , is often regarded as a secondary colonizer of plant tissues and causes several plant diseases including pod and collar rot in soybeans [35], soybean root rot [36], and postharvest fruit rot in oriental melon [37]. It produces apicidins phytotoxic to seedlings and 2-wk-old plants of diverse species [38] and is one of 11 pathogenic Fusarium species listed as quarantine pests in Korea [39]. In addition, it has also been isolated from rotten ginseng roots [5].…”

Section: Discussionmentioning

confidence: 99%

Antagonistic Bacillus species as a biological control of ginseng root rot caused by Fusarium cf. incarnatum

Song

Yun

Kim

2014

Journal of Ginseng Research

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BackgroundThis study aimed to develop a biocontrol system for ginseng root rot caused by Fusarium cf. incarnatum.MethodsIn total, 392 bacteria isolated from ginseng roots and various soils were screened for their antifungal activity against the fungal pathogen, and a bacterial isolate (B2-5) was selected as a promising candidate for the biocontrol because of the strong antagonistic activity of the bacterial cell suspension and culture filtrate against pathogen.ResultsThe bacterial isolate B2-5 displayed an enhanced inhibitory activity against the pathogen mycelial growth with a temperature increase to 25°C, produced no pectinase (related to root rotting) and no critical rot symptoms at low [106 colony-forming units (CFU)/mL] and high (108 CFU/mL) inoculum concentrations. In pot experiments, pretreatment with the bacterial isolate in the presumed optimal time for disease control reduced disease severity significantly with a higher control efficacy at an inoculum concentration of 106 CFU/mL than at 108 CFU/mL. The establishment and colonization ability of the bacterial isolates on the ginseng rhizosphere appeared to be higher when both the bacterial isolate and the pathogen were coinoculated than when the bacterial isolate was inoculated alone, suggesting its target-oriented biocontrol activity against the pathogen. Scanning electron microscopy showed that the pathogen hyphae were twisted and shriveled by the bacterial treatment, which may be a symptom of direct damage by antifungal substances.ConclusionAll of these results suggest that the bacterial isolate has good potential as a microbial agent for the biocontrol of the ginseng root rot caused by F. cf. incarnatum.

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Section: Discussionmentioning

confidence: 99%

Antagonistic Bacillus species as a biological control of ginseng root rot caused by Fusarium cf. incarnatum

Song

Yun

Kim

2014

Journal of Ginseng Research

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“…Fungal genomic DNA was extracted as described previously (Hong et al, 2010). All primers used for PCR amplification were synthesized by Bioneer Corporation (Daejeon, Korea).…”

Section: Methodsmentioning

confidence: 99%

“…All primers used for PCR amplification were synthesized by Bioneer Corporation (Daejeon, Korea). The PCR amplification was performed with the conditions described previously (Hong et al, 2010;Kang et al, 2011). For diagnostic PCR of the G. fujikuroi species complex, three specific primer sets were used: VER1/VER2 and PRO1/PRO2 as F. verticillioides-and F. proliferatumspecific primer sets, respectively , and rp32/rp33 for the FUM1 gene, responsible for fumonisin biosynthesis (Proctor et al, 2004).…”

Section: Methodsmentioning

confidence: 99%

Population Structure of the Gibberella fujikuroi Species Complex Associated with Rice and Corn in Korea

Kim¹,

Kang²,

Kim³

et al. 2012

The Plant Pathology Journal

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“…cucurbitae (F.s.c.) used in this study as; Hantula and Müller, (1997;Zhou et al, (1999); Hong et al, (2010); Kolawole et al, (2015), and proved to be particularly useful for resolving taxonomic conflicts within F. solani strains. In our investigation, the ISSR analysis showed different levels of genetic diversities among the F.s.c.…”

Section: Earlier Established Pcr-rflp Of Ribosomal Intergenic Spacer mentioning

confidence: 99%

Genetic diversity of Fusarium solani f.sp. cucurbitae the causal agent of crown and root rot of watermelon in Tunisia using ISSR markers

Hamdi

Benfradj

Salem

et al. 2019

Novel Research in Microbiology Journal

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Fusarium solani f.sp. cucurbitae (F.s.c.) Snyder and Hansen is one of the most important fungi causing serious damages to the cucurbits production areas in Tunisia. The goal of this investigation was to study the genetic characterization of F. solani f.sp. cucurbitae Tunisian population. Ten isolates of F.s.c. (F.s.c.1 to F.s.c.10) collected from infected watermelon plants in numerous regions of Tunisia; were subjected to PCR identification using ITS1-ITS4 primers pair, and Inter-simple sequence repeat (ISSR) technique. Results obtained confirmed the presence of F. solani f.sp. cucurbitae race 1 in Tunisia. ISSR dendrogram of the ten F.s.c. isolates generated by bi-and trinucleotide primers; and conducted by the unweight pair grouping using mathematical averaging (UPGMA) method from Nei's genetic distance, revealed the presence of similarities between the isolates that ranged from 31 to 84%. The isolates F.s.c.8 (collected from Gabes) and F.s.c.4 (collected from Sidi Bouzid), were genetically dissimilar presenting the lowest similarity coefficient. In contrast; isolates F.s.c.3 (collected from Sfax) and F.s.c.1 (collected from Beja) were similar. Cluster analysis based on UPGMA of the ISSR markers data assembled F.s.c. isolates into two major groups. These results are helpful to develop integrated management and future breeding programs for plant resistance.

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Specific PCR Detection of Four Quarantine Fusarium Species in Korea

Cited by 21 publications

References 13 publications

Antagonistic Bacillus species as a biological control of ginseng root rot caused by Fusarium cf. incarnatum

Antagonistic Bacillus species as a biological control of ginseng root rot caused by Fusarium cf. incarnatum

Population Structure of the Gibberella fujikuroi Species Complex Associated with Rice and Corn in Korea

Genetic diversity of Fusarium solani f.sp. cucurbitae the causal agent of crown and root rot of watermelon in Tunisia using ISSR markers

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