Specific numerical chromosomal aberrations induced by adriamycin

Aly, Magda M.; Othman, Othman E.; Nahas, Soheir M. El

doi:10.1002/(sici)1098-2280(1999)33:2<161::aid-em8>3.0.co;2-m

Cited by 10 publications

(2 citation statements)

References 28 publications

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“…4 ). These in vivo observations are also in line with an earlier in vitro report on human lymphocytes cultured from healthy individuals and cancer patients in whom doxorubicin exposure caused an increase in the trisomies of chromosomes 7 and 17 [40] . Furthermore, Ganapathi et al [41] reported that human leukemia HL-60 cells that carry monosomy 8 as the only karyotypic change acquired 7q21 markers following exposure to doxorubicin.…”

Section: Discussionsupporting

confidence: 90%

Aneugenic Effects of Epirubicin in Somatic and Germinal Cells of Male Mice

et al. 2014

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The ability of the antineoplastic agent epirubicin to induce aneuploidy and meiotic delay in the somatic and germinal cells of male mice was investigated by fluorescence in situ hybridization assay using labeled DNA probes and BrdU-incorporation assay. Mitomycin C and colchicine were used as positive controls for clastogen and aneugen, respectively, and these compounds produced the expected responses. The fluorescence in situ hybridization assay with a centromeric DNA probe for erythrocyte micronuclei showed that epirubicin is not only clastogenic but also aneugenic in somatic cells in vivo. By using the BrdU-incorporation assay, it could be shown that the meiotic delay caused by epirubicin in germ cells was approximately 48 h. Disomic and diploid sperm were shown in epididymal sperm hybridized with DNA probes specific for chromosomes 8, X and Y after epirubicin treatment. The observation that XX- and YY-sperm significantly prevailed over XY-sperm indicates missegregation during the second meiotic division. The results also suggest that earlier prophase stages contribute less to epirubicin-induced aneuploidy. Both the clastogenic and aneugenic potential of epirubicin can give rise to the development of secondary tumors and abnormal reproductive outcomes in cured cancer patients and medical personnel exposed to epirubicin.

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Section: Discussionsupporting

confidence: 90%

Aneugenic Effects of Epirubicin in Somatic and Germinal Cells of Male Mice

et al. 2014

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“…A dose-dependent increase of micronucleus frequency was also reported in the same cell system [ 36 ]. Additionally, DOX aneugenic effect was also observed in lymphocytes from healthy individuals and cancer patients, since an increase in the trisomy of chromosomes 7 and 17, using FISH DNA-specific probes was identified [ 37 ]. To better clarify the aneugenic effect of DOX in complement to the micronucleus analysis, we investigated the integrity of the mitotic apparatus.…”

Section: Discussionmentioning

confidence: 99%

Combined study on clastogenic, aneugenic and apoptotic properties of doxorubicin in human cells in vitro

Chondrou

Trochoutsou

Panayides

et al. 2018

J of Biol Res-Thessaloniki

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BackgroundDoxorubicin is a widely used anticancer drug due to its broad spectrum of antitumor activity. Various mechanisms have been proposed for its cytostatic activity, including DNA intercalation, topoisomerase II inhibition, generation of free radicals and apoptosis. The present study aims to further clarify the cytostatic activity of doxorubicin by its specific effect on (a) DNA damage, (b) micronucleation and (c) apoptosis, using a combination of different methods and cell systems such as human lymphocytes and HL-60 human leukemic cells. DNA lesions were analyzed by the alkaline comet assay in combination with formamidopyrimidine (Fpg) and human 8-oxoguanine (hOGG1) repair enzymes. Micronucleation was investigated by the Cytokinesis-Block Micronucleus assay (CBMN) in combination with Fluorescence In Situ Hybridization analysis. Impairment on mitotic apparatus was investigated by double immunofluorescence of β- and γ-tubulin. Apoptotic cell frequency was determined by the CBMN cytome assay. Complementary to the above, caspase-3 level was investigated by Western blot.ResultsIt was found that doxorubicin generates DNA breakage induced by oxidative damage in DNA bases, which can be repaired by the Fpg and hOGG1 enzymes. Increased micronucleus frequency was identified mainly through chromosome breakage and, at a lesser extent, through chromosome delay. Analysis of mitotic spindle showed disturbance of chromosome orientation and centrosome duplication and/or separation, leading to aneuploidy. Enhanced frequency of apoptotic leukemic cells was also observed. Caspase-3 seems to be involved in the generation of apoptosis.ConclusionsThe aforementioned findings derived from different treatment schedules, doses and time of exposure on primary versus transformed cells extend our knowledge about doxorubicin genotoxicity and contribute to the better understanding of the mechanisms by which doxorubicin induces genotoxic effects on human cells.

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Parameiosis inAspergillus nidulans in response to doxorubicin

Becker

Castro-Prado

2004

Folia Microbiol

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The recombinagenic effect of doxorubicin (an anticancer agent that impairs DNA synthesis and causes chromosome breaks) was used to induce parameiotic events in Aspergillus nidulans. Heterokaryons formed with master strains and uvs mutants were inoculated with and without doxorubicin. Haploid segregants (parameiotics and parents) and aneuploids were selected as heterokaryon-derived visible sectors. Among parameiotic segregants, recombinants by intergenic mitotic crossing-over and recombinants by chromosome-independent segregation were found. Whereas segregants of the former type were obtained only with doxorubicin, those of the latter type were recovered both with and without the drug.

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Specific numerical chromosomal aberrations induced by adriamycin

Cited by 10 publications

References 28 publications

Aneugenic Effects of Epirubicin in Somatic and Germinal Cells of Male Mice

Aneugenic Effects of Epirubicin in Somatic and Germinal Cells of Male Mice

Combined study on clastogenic, aneugenic and apoptotic properties of doxorubicin in human cells in vitro

Parameiosis inAspergillus nidulans in response to doxorubicin

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