Specific nuclear binding of adenosine 3',5'-monophosphate-binding protein complex with subsequent poly(A) RNA synthesis in embryonic chick cartilage.

Burch, Warner M.; Lebovitz, H. E.

doi:10.1172/jci109885

Cited by 11 publications

(4 citation statements)

References 36 publications

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“…4). This observation suggests that cyclic AMP may trigger growth directly by acting on gene transcription [7]. This tachyphylaxis to PTH is similar to the PTH refractiveness in cyclic AMP production in rat calvaria reported by Heersche and Aurbach [25].…”

Section: Discussionsupporting

confidence: 85%

Parathyroid hormone stimulates growth of embryonic chick pelvic cartilagein vitro

Burch

1983

Calcif Tissue Int

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Section: Discussionsupporting

confidence: 85%

Parathyroid hormone stimulates growth of embryonic chick pelvic cartilagein vitro

Burch

1983

Calcif Tissue Int

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“…In vitro incubation of chick embryo pelvic cartilage with butyrylated cyclic AMP derivatives or agents that elevate intracellular cyclic AMP increases amino acid transport (4), and stimulates proteoglycan, total protein and total RNA synthesis (5). The increase in RNA synthesis is coordinate (6) and the stimulation of poly(A) RNA synthesis occurs by direct nuclear effects of a specific cyclic AMP-cytosolic cyclic AMP binding protein complex (7). Studies with developing limb buds in vitro show that cyclic AMP levels rise as mesenchymal cells are differentiating into cartilage (8).…”

Section: Introductionmentioning

confidence: 99%

Adenosine 3',5'-monophosphate: a modulator of embryonic chick cartilage growth.

Burch¹

1981

J. Clin. Invest.

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A B S T R A C T We tested the hypothesis that cyclic AMP plays a significant role in modulating the growth of embryonic chick cartilage by determining whether cyclic AMP levels change in growing embryonic cartilage and whether cyclic AMP could stimulate embryonic cartilage growth in a long term in vitro organ culture. Cyclic AMP levels were low (0.1 pmol/mg wet wt) in 8-d chick embryo pelvic cartilage, and increased progressively through the 11th d of embryonic development at which time they reached a maximum (1.8 pmol/mg wet weight) and thereafter remained constant. We developed an in vitro organ culture system to determine whether cyclic AMP, a factor known to stimulate radiolabeled precursor incorporation into macromolecules in short-term studies does, in fact, stimulate growth of cartilage. Individual pelvic cartilages were isolated from 9-d chick embryos, placed in serum-free medium (BGJb-FJ modification) and incubated for 3 to 5 d during which time they increased in size (39 and 60% in length, respectively), wet weight (90 and 141%, respectively), and content of total soluble protein (30 and 48%, respectively). N6-monobutyryl cyclic AMP (BtcAMP) added to the medium caused a dose-dependent (0.05 to 1.0 mM) stimulation of growth. After 3 d of incubation, 1.0 mM BtcAMP increased wet weight (125%), [14C]leucine incorporation into protein (75%), and [3H]thymidine incorporation into DNA (48%) compared with control cartilages incubated in medium alone. 1-methyl-3-isobutyl xanthine, a phosphodiesterase inhibitor, also increased cartilage growth above control while sodium butyrate, AMP, and ATP had no effect. Histological examination of cartilage grown in medium was similar to that of cartilage developing in ovo, whereas, cartilage grown in medium containing BtcAMP showed marked hypercellularity with many immature chondrocytes. Our observations are compatible with the hypothesis that cyclic AMP can significantly modulate the growth of embryonic cartilage.

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“…The presence of CAMP-dependent protein kinase in prechondrogenic limb cells demonstrates further that the effector system through which cAMP is thought to exert its effects on genomic activity (Earp and Steiner, 1978) is intact and is as easily activated in undifferentiated cells as in the more differentiated phenotypes. While PKA activity has been reported in embryonic cartilage (Burch and Lebovitz, 1980), this is to our knowledge the first report of its presence and characteristics in prechondrogenic limb cells. Our preliminary studies on its distribution indicate that the major activity is soluble, as has been reported in many other differentiated cell types (Krebs and Beavo, 1979).…”

Section: Discussionmentioning

confidence: 65%

Limb development in chick embryos: Cyclic AMP‐dependent protein kinase activity, cyclic AMP, and prostaglandin concentrations during cytodifferentiation and morphogenesis

Smales

Biddulph

1985

Journal Cellular Physiology

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The effects of prostaglandin E2 (PGE2) on cyclic AMP (cAMP) concentrations of chick limb bud cells obtained from limbs at various stages of development were investigated. In addition, endogenous concentrations of PGE2 were examined in whole limbs from comparable stages. Prior to either chondrogenesis or myogenesis (stages 20-23), cells were more responsive to PGE2, in terms of cAMP levels, than those of differentiated phenotypes, obtained at stages 25-28. This greater responsiveness to PGE2 of undifferentiated cells was correlated with endogenous concentrations of PGE2 which were significantly higher in undifferentiated limbs than in limbs containing differentiated cartilage and muscle. Cyclic AMP-dependent protein kinase (PKA) activity was detectable in cell homogenates at each stage examined and did not appear to change in cAMP dependency at any stage. The majority (80-85%) of total enzyme activity was localized in soluble fractions of cell homogenates while the residual activity was localized to membrane-enriched, particulate fractions. The results demonstrate that both responsiveness of limb mesenchyme to PGE2 and endogenous concentrations of PGE2 are maximal prior to cytodifferentiation of limb tissues. The presence of cAMP-dependent protein kinase in these undifferentiated cells supports a regulatory role for both PGE2 and a cAMP-protein phosphorylation system in the differentiation of limb tissues.

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Specific nuclear binding of adenosine 3',5'-monophosphate-binding protein complex with subsequent poly(A) RNA synthesis in embryonic chick cartilage.

Abstract: A B S T R A C T

Cited by 11 publications

References 36 publications

Parathyroid hormone stimulates growth of embryonic chick pelvic cartilagein vitro

Parathyroid hormone stimulates growth of embryonic chick pelvic cartilagein vitro

Adenosine 3',5'-monophosphate: a modulator of embryonic chick cartilage growth.

Limb development in chick embryos: Cyclic AMP‐dependent protein kinase activity, cyclic AMP, and prostaglandin concentrations during cytodifferentiation and morphogenesis

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