Specific neural phase relation of serotonin and dopamine modulate the testicular activity in Japanese quail

Banerjee, S.; Chaturvedi, Chandra Mohini

doi:10.1002/jcp.27104

Cited by 7 publications

(8 citation statements)

References 59 publications

(122 reference statements)

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“…However, before concluding this and validating the earlier ideas, we must consider the specificity and sensitivity of the techniques that we have used. Although histochemistry may be considered an old method, this analysis has been used to identify not only 5-HT but also adrenaline, noradrenaline, and dopamine in testes, raising the possibility that other neurotransmitters could directly participate in the regulation of spermatogenesis in adult rats (Meizel, 2004;Hökfelt, 2010;Banerjee and Chaturvedi, 2019). The monoclonal antibodies against 5-HT that we used did not allow us to exclude the possibility of the presence of another indolamine, i.e., evidence of the local synthesis of melatonin in rat testes has been reported (Tijmes et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Indolaminergic System in Adult Rat Testes: Evidence for a Local Serotonin System

et al. 2021

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Serotonin (5-HT) is member of a family of indolamine molecules that participate in a wide variety of biological processes. Despite its important role in the regulation of local blood systems, little is known about the physiological function of 5-HT in reproductive organs, its functional implications, and its role in the reproduction of mammals. In the present work, we evaluated the localization and distribution of 5-HT (using histochemical analysis of indolamines) and different components of the serotoninergic system in rat testes. We detected local synthesis and degradation through immunofluorescence and western blot analyses against the TPH1, MAOA, 5-HTT, and VMAT1 serotonin transporters. We also identified the localization and distribution of the 5-HT1B, 5-HT2A, and 5-HT3A receptors. RT-PCR results showed the presence of the Tph1, Maoa, Slc6a4, and Htr3a genes in testes and in the brain stem (Tph1 was used as a negative control). High-performance liquid chromatography was used to determine the presence of 5-HT and the activity of tryptophan hydroxylase in testes homogenates in vitro. Our observations suggest that TPH1 activity and local 5-HT synthesis befall in rat testes. We propose that 5-HT could participate in the regulation of testosterone synthesis and in the spermatogenesis process via local serotoninergic system. However, more studies are needed before concluding that rat testes, or those of other mammals, contain an active form of tryptophan hydroxylase and produce 5-HT.

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Section: Discussionmentioning

confidence: 99%

Indolaminergic System in Adult Rat Testes: Evidence for a Local Serotonin System

et al. 2021

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“…The results of the present study suggested that OPN5 played an important role in the regulation of avian reproductive activity by mediating photoperiod in non-seasonal breeding avian. OPN5 affects TSH, which activates the DIO2/DIO3 conversion system ( Ono et al, 2008 ), which in turn affects the GnRH/GnIH system and the GnRHR/GnIHR system, altering the expression of related reproductive genes and causing changes in the corresponding reproductive hormones, thus regulating the ovarian functional system ( Banerjee and Chaturvedi, 2018 ). Furthermore, OPN5 has been shown to mediate light signaling and cause a drift in rhythmic phase, which in turn is involved in the regulation of animal biological rhythms and reproductive functions, such as the association of altered sexual behavior and GnRH release ( Prasad et al, 2015 ; Han et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

OPN5 Regulating Mechanism of Follicle Development Through the TSH-DIO2/DIO3 Pathway in Mountain Ducks Under Different Photoperiods

et al. 2022

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Photoperiod is an important environmental factor that influence seasonal reproduction behavior in bird. Birds translates photoperiodic information into neuroendocrine signals through deep brain photoreceptors (DBPs). OPN5 has been considered as candidate DBPs involving in regulation of seasonal reproduction in birds. However, little is known about the effect of OPN5 in non-seasonal breeding birds. Thus, we pondered on whether OPN5 regulating follicular development through TSH-DIO2/DIO3 system responds to different photoperiods in non-seasonal laying ducks. As an ideal non-seasonal breeding bird, a total of 120 mountain ducks were randomly divided into three groups and treated respectively to a different photoperiod: group S (8 L:18D), group C (17 L:7D), and group L (24 L:0D). The ducks were caged in a fully enclosed shelter with the same feeding conditions for each group, free water and limited feeding (150 g per duck each day). Samples were collected from each group at d 0, d 5, d 8, d 20, and d 35 (n = 8). The ducks in 24 h photoperiod had the highest laying rate and the lowest feed-to-egg ratio, while the ducks in 8 h photoperiod had the lowest laying rate and the highest feed-to-egg ratio. Long-day photoperiod for 24 h significantly increased the ovarian index and GnRH, LH, E2, and P4 levels in serum; short-day photoperiod for 8 h increased PRL and testosterone levels in serum. Compared with 8 h photoperiod, long-day photoperiod significantly or highly significantly increased the mRNA level and protein expression of OPN5 in the hypothalamus of long-day photoperiod on d 35 (p < 0.05). The gene or protein expression patterns of GnRH, TRH, TSHβ, DIO2, THRβ, Vasoactive Intestinal peptide(IP), and PRL were positively correlated with OPN5, whereas the gene expression patterns of GnIH and DI O 3 were negatively correlated with OPN5. The results revealed that OPN5 mediated the effect of light on follicular development through the TSH-DIO2/DIO3 pathway, the expression of OPN5 increased with light duration and improved the efficiency of the HPG axis to promote follicular development in mountain ducks.

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“…Testosterone treatment in some studies is showed a strong influence on SERT expression (Kranz et al, 2015), whereas our data are almost absent in S-to-L groups and only reduced SERT expression in L-to-S groups. Several studies concerned with the association of neurotransmitters, especially serotonin, with seasonal reproduction have been reported (Lambert et al, 2002;Banerjee and Chaturvedi, 2019). Moreover, numerous studies have found that steroidogenesis is closely associated with seasonal reproduction, but the correlations between neurotransmitter and steroidogenesis are still unclear.…”

Section: Discussionmentioning

confidence: 99%

Photoperiodic Changes in Both Hypothalamus Neurotransmitters and Circulating Gonadal Steroids Metabolomic Profiles in Relation to Seasonal Reproduction in Male Quail

Jiang

Liu

et al. 2022

Front. Physiol.

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Both hypothalamic neurotransmitters and serum steroid hormones are impacted by photoperiod and have effects on physiology and seasonal reproductive. However, the relationship between circulating gonadal steroids and hypothalamic neurotransmitters underlying different photoperiod is still unclear. To further understand the crosstalk of neurotransmitters and steroids in seasonal reproduction, metabolic changes of 27 neurotransmitters concentrated in hypothalamus tissues and 42 steroids hormones in serum were assessed during two artificial photoperiodic programs. The results showed that photoperiod induce testicular atrophy and recrudescence. In L-to-S groups, significantly decreased levels of testosterone concentration were found in serum (P < 0.001) and increased 11-Dehydrocorticosterone (P < 0.05); Testosterone were almost undetectable at SD_14d. In addition, the hypothalamus exhibited significantly increased arginine and 4-aminobutyric acid (GABA) concentration and decreased serotonin and epinephrine content (P < 0.01 or P < 0.05). Accordingly, serum testosterone and androstenedione became detectable at LD_3d in the S-to-L group and were markedly increase at LD_7d. Furthermore, Serum androstenedione showed a significant increase with long light expose (P < 0.01). Additionally, the hypothalamus exhibited both significantly increased L.Tryptophan and phenylalanine concentration, as well as decreased L-glutamine and L-glutamine.acid content (P < 0.01 or P < 0.05). Serotonin metabolism showed significant differences between L-to-S group and S-to-L group. Furthermore, in the correlation analysis, serum testosterone had a positive correlation with 5-Hydroxyindole-3-acetic acid (5-HIAA), while Androstenedione was significantly negative with L.Tryptophan in L-to-S (P < 0.05). However, in S-to-L group, serum testosterone showed strong negative correlation with both serotonin and 5-HIAA (P < 0.05), but positive correlation with L.Tryptophan (P < 0.01), while Androstenedione was significantly negative correlation with both serotonin (P < 0.05) and L-Glutamine (P < 0.01). Photoperiod also had significant effects on the mRNA expression. We found significant differences in gene expression patterns of both serotonin signaling and steroid biosynthesis, while MAOB, NR5A1, and 3β-HSD showed an opposite tendency between two groups. Taken together, our results revealed that circulating gonadal steroids and hypothalamic neurotransmitters were significantly impact quail’s seasonal reproduction. Circulating gonadal steroids have different effects on neurotransmitter at different photoperiodism, which may coordinately influence the seasonal reproduction of quails.

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Specific neural phase relation of serotonin and dopamine modulate the testicular activity in Japanese quail

Cited by 7 publications

References 59 publications

Indolaminergic System in Adult Rat Testes: Evidence for a Local Serotonin System

Indolaminergic System in Adult Rat Testes: Evidence for a Local Serotonin System

OPN5 Regulating Mechanism of Follicle Development Through the TSH-DIO2/DIO3 Pathway in Mountain Ducks Under Different Photoperiods

Photoperiodic Changes in Both Hypothalamus Neurotransmitters and Circulating Gonadal Steroids Metabolomic Profiles in Relation to Seasonal Reproduction in Male Quail

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