Specific MR imaging of human lymphocytes by monoclonal antibody‐guided dextran‐magnetite particles

Bulte, Jeff W.M.; Hoekstra, Y; Kamman, R. L.; Magin, Richard L.; Webb, Andrew; Briggs, Richard W.; Go, K.G.; Hulstaert, C.E.; Miltenyi, Stefan; Leij, Lfmh de

doi:10.1002/mrm.1910250115

Cited by 133 publications

(82 citation statements)

References 26 publications

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“…Monoclonal antibodies (mAbs) were the first targeting agents to exploit molecular recognition to deliver MNPs [125,126,127,128] and continue to be widely used due to their high specificity. Recently, the development of Herceptin ™ , an FDA-approved mAb to the HER2/neu (erbB2) receptor, has made it a popular targeting agent for nanoparticles [129,130].…”

Section: Active Targetingmentioning

confidence: 99%

Magnetic nanoparticles in MR imaging and drug delivery

Sun

Lee

Zhang

2008

Advanced Drug Delivery Reviews

2,287

1,507

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Magnetic nanoparticles (MNPs) possess unique magnetic properties and the ability to function at the cellular and molecular level of biological interactions making them an attractive platform as contrast agents for magnetic resonance imaging (MRI) and as carriers for drug delivery. Recent advances in nanotechnology have improved the ability to specifically tailor the features and properties of MNPs for these biomedical applications. To better address specific clinical needs, MNPs with higher magnetic moments, non-fouling surfaces, and increased functionalities are now being developed for applications in the detection, diagnosis, and treatment of malignant tumors, cardiovascular disease, and neurological disease. Through the incorporation of highly specific targeting agents and other functional ligands, such as fluorophores and permeation enhancers, the applicability and efficacy of these MNPs have greatly increased. This review provides a background on applications of MNPs as MR imaging contrast agents and as carriers for drug delivery and an overview of the recent developments in this area of research.

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Section: Active Targetingmentioning

confidence: 99%

Magnetic nanoparticles in MR imaging and drug delivery

Sun

Lee

Zhang

2008

Advanced Drug Delivery Reviews

2,287

1,507

View full text Add to dashboard Cite

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“…MRI is an appealing noninvasive approach for early cancer diagnostics and therapeutics [10]. However, in clinical diagnosis, it is often the case that the diseased and healthy tissues are similar in composition and MRI technology alone will not always differentiate the two.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and characterization of PVP-functionalized superparamagnetic Fe3O4 nanoparticles as an MRI contrast agent

Arsalani¹,

Fattahi²,

Nazarpoor³

2010

Express Polym. Lett.

225

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Abstract. The magnetite (Fe3O4) nanoparticles (MNPs) coated with poly(N-vinyl pyrrolidone) (PVP) via covalent bonds were prepared as T2 contrast agent for magnetic resonance imaging (MRI). The surface of MNPs was first coated with 3-(trimethoxysilyl) propyl methacrylate (silan A) by a silanization reaction to introduce reactive vinyl groups onto the surface, then poly(N-vinyl pyrrolidone) was grafted onto the surface of modified-MNPs via surface-initiated radical polymerization. The obtained nanoparticles were characterized by FT-IR (Fourier transform infrared spectroscopy), XRD (X-ray diffraction), TEM (transmission electron microscopy), VSM (vibrating sample magnetometer), and TGA (thermogravimetric analysis). The MNPs had an average size of 14 nm and exhibited superparamagnetism and high saturation magnetization at room temperature. T2-weighted MRI images of PVP-grafted MNPs showed that the magnetic resonance signal is enhanced significantly with increasing nanoparticle concentration in water. The r1 and r2 values per millimole Fe, and r2/r1 value of the PVP-grafted MNPs were calculated to be 2.6 , 72.1, and 28.1(mmol/l) -1 ·s -1 , respectively. These results indicate that the PVP-grafted MNPs have great potential for application in MRI as a T2 contrast agent. Vol.4, No.6 (2010) 329-338 Available online at www.expresspolymlett.com DOI: 10.3144/expresspolymlett.2010.42 region that they are accumulated, and hence cause negative contrast and provide a dark state in the image where the compounds are accumulated [15]. However, the direct use of magnetic nanoparticles as in vivo MRI contrast agent results in biofouling of the particles in blood plasma and formation of aggregates that are quickly sequestered by cells of the reticular endothelial system (RES) such as macrophages [16,17]. Furthermore, aggregated nanoparticles change their superparamagnetic response [7]. Therefore, in order to minimize biofouling and aggregation of particles and escape from the RES for longer circulation times, the nanoparticles are usually coated with a layer of hydrophilic and biocompatible polymer such as dextran [18], dendrimers [19], poly(ethylene glycol) (PEG) [20], and poly(vinyl pyrrolidone) (PVP) [21][22][23]. Of synthetic polymers, PVP is water-soluble, non-charged, non-toxic, and is used in various medical applications [24]. While there is a potential concern about covalent interaction between hydrophilic polymers and magnetic nanoparticles in order to increase their stability in physiological medium [25][26][27][28][29], PVP coating on Fe 3 O 4 nanoparticles in all previous works has been achieved through noncovalent interaction. Now, polymers grafting of magnetic nanoparticles is one of the most attractive methods of surface modification [30,31]. In the present study, we carried out chemical synthesis and characterization of PVP-functionalized magnetite (Fe 3 O 4 ) nanoparticles. Since the PVP was bonded to the surface of magnetite nanoparticles through covalent bonds, the prepared magnetic fluid (ferrofluid) was ve...

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“…The strategy of targeting iron-oxide nanoparticles to selected receptors using mAb has been used to image lymphocytes in culture; a biotinylated anti-lymphocyte-directed mAb was conjugated to a biotinylated dextran-coated SPIO with a streptavidin linker, generating selective negative contrast enhancement of lymphocyte suspensions [21]. Kang et al [22] have imaged E-selectin expression in human endothelial cells by conjugating a F(ab′)2 fragment of the anti-human-E-selectin mAb to cross-linked iron oxide (CLIO) nanoparticles.…”

Section: T 2 Contrast Agents-sincementioning

confidence: 99%

Molecular Imaging of Cancer: Applications of Magnetic Resonance Methods

et al. 2005

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Cancer is a complex disease exhibiting a host of phenotypic diversities. Noninvasive multinuclear magnetic resonance imaging (MRI) and spectroscopic imaging (MRSI) provide an array of capabilities to characterize and understand several of the vascular, metabolic, and physiological characteristics unique to cancer. The availability of targeted contrast agents has widened the scope of MR techniques to include the detection of receptor and gene expression. In this paper, we have highlighted the application of several MR techniques in imaging and understanding cancer.

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Specific MR imaging of human lymphocytes by monoclonal antibody‐guided dextran‐magnetite particles

Cited by 133 publications

References 26 publications

Magnetic nanoparticles in MR imaging and drug delivery

Magnetic nanoparticles in MR imaging and drug delivery

Synthesis and characterization of PVP-functionalized superparamagnetic Fe3O4 nanoparticles as an MRI contrast agent

Molecular Imaging of Cancer: Applications of Magnetic Resonance Methods

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