Specific molten globule conformation of stem bromelain at alkaline pH

Dave, Sandeep; Mahajan, Sahil; Chandra, Vemika; Dkhar, Hedwin Kitdorlang; Sambhavi, None; Gupta, Pawan

doi:10.1016/j.abb.2010.05.005

Cited by 16 publications

(15 citation statements)

References 38 publications

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“…Previous studies in our laboratory demonstrated that SBM adopts a specific molten globule (SMG) conformation at pH 10, near its isoelectric point [3]. In the present study, we demonstrate the formation of an MG-like state characterized by disordered sidechain interactions but with considerable secondary structure when the SMG is subjected to 30% TFE.…”

Section: Introductionmentioning

confidence: 52%

“…We used ANS to study the TFE-induced structural features of the SMG form of SBM. ANS has been shown to bind more effectively to the SMG form than to the native conformation (i.e., at pH 7) [3]. In the present study, TFE-induced conformational changes in the SMG led to an increase in ANS binding ( Fig.…”

Section: Effect Of Tfe On Ans Binding To the Smg Form Of Sbmmentioning

confidence: 67%

“…Previously, SBM at pH 10 was shown to exist in a pre-SMG conformation that retained its native-like secondary and tertiary content, but in which its hydrophobic groups were more accessible [3]. Changes in the SMG's secondary and tertiary structure as a function of TFE concentration were monitored by far-and near-UV CD (Fig.…”

Section: Effects Of Tfe On the CD Spectra Of The Smg Form Of Sbmmentioning

confidence: 99%

“…One such intermediate is the MG, a compact collapsed form of a protein with a pronounced secondary structure but lacking rigid tertiary structure [5,[22][23][24]. However, in several instances, the MG has been shown to possess well-defined tertiary contacts, i.e., a pre-molten globule state [3,5,25,26]. Comparing these structural intermediates of a protein could provide clues to the nature of protein folding [23,27].…”

Section: Introductionmentioning

confidence: 97%

“…However, the protein conformation can be influenced by many factors including, but not limited to, solvents, mechanical forces, chemical denaturants, and pH. Extremes of pH can induce pre-molten globule states, molten globules (MGs), partially folded intermediates, and aggregates of native proteins [2][3][4][5][6][7][8]. Protein folding at alkaline pH has not been extensively characterized, and even less studied is the effect of fluoroalcohols on these alkali-induced states [8].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Trifluoroethanol stabilizes the molten globule state and induces non-amyloidic turbidity in stem bromelain near its isoelectric point

Dave

Mahajan

Chandra

et al. 2011

International Journal of Biological Macromolecules

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Section: Introductionmentioning

confidence: 52%

Section: Effect Of Tfe On Ans Binding To the Smg Form Of Sbmmentioning

confidence: 67%

Section: Effects Of Tfe On the CD Spectra Of The Smg Form Of Sbmmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Trifluoroethanol stabilizes the molten globule state and induces non-amyloidic turbidity in stem bromelain near its isoelectric point

Dave

Mahajan

Chandra

et al. 2011

International Journal of Biological Macromolecules

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Heterologous expression of the plant cysteine protease bromelain and its inhibitor in Pichia pastoris

Luniak

Meiser

Burkart³

et al. 2016

Biotechnology Progress

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Expression of proteases in heterologous hosts remains an ambitious challenge due to severe problems associated with digestion of host proteins. On the other hand, proteases are broadly used in industrial applications and resemble promising drug candidates. Bromelain is an herbal drug that is medicinally used for treatment of oedematous swellings and inflammatory conditions and consists in large part of proteolytic enzymes. Even though various experiments underline the requirement of active cysteine proteases for biological activity, so far no investigation succeeded to clearly clarify the pharmacological mode of action of bromelain. The potential role of proteases themselves and other molecules of this multi-component extract currently remain largely unknown or ill defined. Here, we set out to express several bromelain cysteine proteases as well as a bromelain inhibitor molecule in order to gain defined molecular entities for subsequent studies. After cloning the genes from its natural source Ananas comosus (pineapple plant) into Pichia pastoris and subsequent fermentation and purification, we obtained active protease and inhibitor molecules which were subsequently biochemically characterized. Employing purified bromelain fractions paves the way for further elucidation of pharmacological activities of this natural product. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:54-65, 2017.

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Synthesis of Polypeptides

Tsuchiya

Miyamoto

et al. 2019

Green Chemistry and Sustainable Technology

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Specific molten globule conformation of stem bromelain at alkaline pH

Cited by 16 publications

References 38 publications

Trifluoroethanol stabilizes the molten globule state and induces non-amyloidic turbidity in stem bromelain near its isoelectric point

Trifluoroethanol stabilizes the molten globule state and induces non-amyloidic turbidity in stem bromelain near its isoelectric point

Heterologous expression of the plant cysteine protease bromelain and its inhibitor in Pichia pastoris

Synthesis of Polypeptides

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