Specific molecular detection of Phytophthora sojae using conventional and real-time PCR

Bienapfl, J. C.; Malvick, Dean; Percich, J. A.

doi:10.1016/j.funbio.2011.05.007

Cited by 31 publications

(21 citation statements)

References 12 publications

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“…Canada and multiple Midwest states in the USA [12,13]. It causes discoloration and rotting of lateral root and internal discoloration and rotting of the taproot.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial Genome Sequence ofPhytophthora sansomeanaand Comparative Analysis ofPhytophthoraMitochondrial Genomes

Cai

Scofield

2020

Preprint

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ABSTRACTPhytophthora sansomeana infects soybean and causes root rot. It was recently separated from the species complex P. megasperma sensu lato. In this study, we sequenced and annotated its complete mitochondrial genome and compared it to that of nine other Phytophthora species. The genome was assembled into a circular molecule of 39,618 bp with a 22.03% G+C content. Forty-two protein coding genes, 25 tRNA genes and two rRNA genes were annotated in this genome. The protein coding genes include 14 genes in the respiratory complexes, four ATP synthetase genes, 16 ribosomal proteins genes, a tatC translocase gene, six conserved ORFs and a unique orf402. The tRNA genes encode tRNAs for 19 amino acids. Comparison among mitochondrial genomes of 10 Phytophthora species revealed three inversions, each covering multiple genes. These genomes were conserved in gene content with few exceptions. A 3’ truncated atp9 gene was found in P. nicotianae. All 10 Phytophthora species, as well as other oomycetes and stramenopiles, lacked tRNA genes for threonine in their mitochondria. Phylogenomic analysis using the mitochondrial genomes supported or enhanced previous findings of the phylogeny of Phytophthora spp.

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“…Canada and multiple Midwest states in the USA [12,13]. It causes discoloration and rotting of lateral root and internal discoloration and rotting of the taproot.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial Genome Sequence ofPhytophthora sansomeanaand Comparative Analysis ofPhytophthoraMitochondrial Genomes

Cai

Scofield

2020

Preprint

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“…Previously included in the species complex P. megasperma sensu lato, it is morphologically similar to but phylogenetically distinct from P. megasperma sensu strictu. P. sansomeana was isolated from soybean in Indiana in 1990 [14] and has now been reported in China [15], Canada and multiple Midwest states in the USA [16,17]. It lives in the soil, infects soybean roots, and causes discoloration and rotting of lateral root and internal discoloration and rotting of the taproot.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial genome sequence of Phytophthora sansomeana and comparative analysis of Phytophthora mitochondrial genomes

Cai

Scofield

2020

PLoS ONE

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Phytophthora sansomeana infects soybean and causes root rot. It was recently separated from the species complex P. megasperma sensu lato. In this study, we sequenced and annotated its complete mitochondrial genome and compared it to that of nine other Phytophthora species. The genome was assembled into a circular molecule of 39,618 bp with a 22.03% G+C content. Forty-two protein coding genes, 25 tRNA genes and two rRNA genes were annotated in this genome. The protein coding genes include 14 genes in the respiratory complexes, four ATP synthase genes, 16 ribosomal proteins genes, a tatC translocase gene, six conserved ORFs and a unique orf402. The tRNA genes encode tRNAs for 19 amino acids. Comparison among mitochondrial genomes of 10 Phytophthora species revealed three inversions, each covering multiple genes. These genomes were conserved in gene content with few exceptions. A 3' truncated atp9 gene was found in P. nicotianae. All 10 Phytophthora species, as well as other oomycetes and stramenopiles, lacked tRNA genes for threonine in their mitochondria. Phylogenomic analysis using the mitochondrial genomes supported or enhanced previous findings of the phylogeny of Phytophthora spp.

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“…A PCR analysis using a species-specific primer pair (PSOJF1 and PSOJR1) ( Bienapfl et al, 2011 ) confirmed that the expected amplicon was produced for all isolates except for four isolates provided by the Hokuriku Research Center, CARC/NARO. The four isolates from the Hokuriku Research Center have been already identified as P. sojae by means of morphological observation, PCR-based molecular characterization, and inoculation of soybean seedlings ( Moriwaki, 2010 ).…”

Section: Methodsmentioning

confidence: 82%

Variation in the Resistance of Japanese Soybean Cultivars to Phytophthora Root and Stem Rot during the Early Plant Growth Stages and the Effects of a Fungicide Seed Treatment

et al. 2019

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Specific molecular detection of Phytophthora sojae using conventional and real-time PCR

Cited by 31 publications

References 12 publications

Mitochondrial Genome Sequence ofPhytophthora sansomeanaand Comparative Analysis ofPhytophthoraMitochondrial Genomes

Mitochondrial Genome Sequence ofPhytophthora sansomeanaand Comparative Analysis ofPhytophthoraMitochondrial Genomes

Mitochondrial genome sequence of Phytophthora sansomeana and comparative analysis of Phytophthora mitochondrial genomes

Variation in the Resistance of Japanese Soybean Cultivars to Phytophthora Root and Stem Rot during the Early Plant Growth Stages and the Effects of a Fungicide Seed Treatment

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