Specific microRNAs Regulate Dental Pulp Stem Cell Behavior

Kulthanaamondhita, Promphakkon; Kornsuthisopon, Chatvadee; Photichailert, Suphalak; Manokawinchoke, Jeeranan; Limraksasin, Phoonsuk; Osathanon, Thanaphum

doi:10.1016/j.joen.2022.02.012

Cited by 12 publications

(5 citation statements)

References 80 publications

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“…In addition, this study was only able to evaluate DPSCs from a single repository; therefore, these results should be confirmed using other DPSC biorepositories and ex vivo samples [ 62 ]. Finally, other factors, such as the expression of microRNAs, may be important factors to consider in future studies of DPSC responsiveness to the ECM as in other recent studies of MSCs [ 63 , 64 , 65 ].…”

Section: Discussionmentioning

confidence: 99%

Differential Effects of Extracellular Matrix Glycoproteins Fibronectin and Laminin-5 on Dental Pulp Stem Cell Phenotypes and Responsiveness

Lee

Bae

Kim

et al. 2023

JFB

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Dental pulp stem cells (DPSCs) are mesenchymal stem cells (MSCs) with the potential to differentiate in a limited number of other tissue types. Some evidence has suggested the modulation of DPSC growth may be mediated, in part, by exogenous extracellular matrix (ECM) glycoproteins, including fibronectin (FN) and laminin-5 (LN5). Although preliminary research suggests that some ECM glycoproteins may work as functional biomaterials to modulate DPSC growth responses, the primary goal of this project is to determine the specific effects of FN and LN5 on DPSC growth and viability. Using an existing DPSC repository, n = 16 DPSC isolates were cultured and 96-well growth assays were performed, which revealed FN, LN5 and the combination of these were sufficient to induce statistically significant changes in growth among five (n = 5) DPSC isolates. In addition, the administration of FN (either alone or in combination) was sufficient to induce the expression of alkaline phosphatase (ALP) and dentin sialophosphoprotein (DSPP), while LN5 induced the expression of ALP only, suggesting differential responsiveness among DPSCs. Moreover, these responses appeared to correlate with the expression of MSC biomarkers NANOG, Oct4 and Sox2. These results add to the growing body of evidence suggesting that functional biomaterials, such as ECM glycoproteins FN and LN5, are sufficient to induce phenotypic and differentiation-specific effects in a specific subset of DPSC isolates. More research will be needed to determine which biomarkers or additional factors are necessary and sufficient to induce the differentiation and development of DPSCs ex vivo and in vitro for biomedical applications.

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Section: Discussionmentioning

confidence: 99%

Differential Effects of Extracellular Matrix Glycoproteins Fibronectin and Laminin-5 on Dental Pulp Stem Cell Phenotypes and Responsiveness

Lee

Bae

Kim

et al. 2023

JFB

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“…As small non-coding RNAs, miRNAs play a crucial role in orchestrating post-transcriptional gene expression and intricately influence various cellular processes, including angiogenesis. Leveraging the angiogenic processes of miRNAs can positively influence vascular network formation and dental pulp regeneration [10,11]. Despite their potential for regulating cellular processes, miRNAs have certain inherent shortcomings.…”

Section: Introductionmentioning

confidence: 99%

Chemically Modified MicroRNA Delivery via DNA Tetrahedral Frameworks for Dental Pulp Regeneration

Wei,

Xu,

Zhou

et al. 2024

Preprint

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Dental pulp regeneration is a promising strategy for addressing tooth disorders. Incorporating this strategy involves the fundamental challenge of establishing functional vascular networks using dental pulp stem cells (DPSCs) to support tissue regeneration. Current therapeutic approaches lack efficient and stable methods for activating DPSCs. In the study, we used a chemically modified microRNA (miRNA)-loaded tetrahedral-framework nucleic acid nanostructure to promote DPSC-mediated angiogenesis and dental pulp regeneration. Incorporating chemically modified miR-126-3p into tetrahedral DNA nanostructures (miR@TDNs) represents a notable advancement in the stability and efficacy of miRNA delivery into DPSCs. These nanostructures enhanced DPSC proliferation , migration, and upregulated angiogenesis-related genes, enhancing their paracrine signaling effects on endothelial cells. This enhanced effect was substantiated by improvements in endothelial cell tube formation, migration, and gene expression. Moreover, in vivo investigations employing matrigel plug assays and 1 ectopic dental pulp transplantation confirmed the potential of miR@TDNs in promoting angiogenesis and facilitating dental pulp regeneration. Our findings demonstrated the potential of chemically modified miRNA-loaded nucleic acid nanostructures in enhancing DPSC-mediated angiogenesis and supporting dental pulp regeneration. These results highlighted the promising role of chemically modified nucleic acid-based delivery systems as therapeutic agents in regenerative dentistry and tissue engineering.

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“…MicroRNAs (miRNAs) are one of the molecules involved in the regulation of biological processes of the cells through the degradation of mRNA. MiRNAs have been shown to modulate the stemness and differentiation of various types of mesenchymal stem cells including PDLSCs [ 13 , 14 ]. During the differentiation of human PDLSCs stimulated by various osteogenic conditions, the differential expression of miRNAs was observed.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA expression in JAG1/Notch-activated periodontal ligament stem cells

Kulthanaamondhita,

Kornsuthisopon,

Chansaenroj

et al. 2024

BDJ Open

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Objectives The study explored the expression profile of miRNAs in Notch-activated periodontal ligament stem cells (PDLSCs) and examined their potential cellular targets. Methods PDLSCs were cultured and treated with indirect immobilized Jagged1. The miRNA expression profile was examined using NanoString analysis. Bioinformatic analysis was performed together with enrichment, and miRNA expression was evaluated and validated using a quantitative polymerase chain reaction (qPCR). Results A total of 26 miRNAs were differentially expressed in Jagged1 treated PDLSCs compared with the controls. Pathway analysis revealed that altered miRNAs were significantly associated with the transforming growth factor β (TGF-β) signaling pathway. Target prediction analysis demonstrated that 11,170 genes as predictable targets of these altered miRNAs. Enrichment of predicted target genes revealed that they were related to ErbB, Ras and MAPK signaling pathways and small GTPase transduction. Conclusions The research concludes that several miRNAs are differentially expressed in jagged-1 treated PDLSCs. In translational terms the differential functionality of these miRNAs offer promise for the development of targeted regenerative materials that are necessary for managing lost tissue replacement in periodontal diseases.

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Specific microRNAs Regulate Dental Pulp Stem Cell Behavior

Cited by 12 publications

References 80 publications

Differential Effects of Extracellular Matrix Glycoproteins Fibronectin and Laminin-5 on Dental Pulp Stem Cell Phenotypes and Responsiveness

Differential Effects of Extracellular Matrix Glycoproteins Fibronectin and Laminin-5 on Dental Pulp Stem Cell Phenotypes and Responsiveness

Chemically Modified MicroRNA Delivery via DNA Tetrahedral Frameworks for Dental Pulp Regeneration

MicroRNA expression in JAG1/Notch-activated periodontal ligament stem cells

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