Specific Maltose Derivatives Modulate the Swarming Motility of Nonswarming Mutant and Inhibit Bacterial Adhesion and Biofilm Formation by <i>Pseudomonas aeruginosa</i>

Shetye, Gauri; Singh, Nischal; Chen, Jia; Nguyen, Chan D.K.; Wang, Guirong; Luk, Yan Yeung

doi:10.1002/cbic.201402093

Cited by 18 publications

(15 citation statements)

References 62 publications

(95 reference statements)

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“…This is particularly significant as maltose and its derivatives have been linked to adhesion in other organisms. For example, maltose has been shown to inhibit adhesion and biofilm formation in Pseudomonas aeruginosa and enhance adhesion to epithelial cells in Candida albicans [39,40]. In fact, the deletion of an Hbt.…”

Section: Resultsmentioning

confidence: 99%

Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants

Legerme

Yang

Esquivel

et al. 2016

Life

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Archaea, like bacteria, use type IV pili to facilitate surface adhesion. Moreover, archaeal flagella—structures required for motility—share a common ancestry with type IV pili. While the characterization of archaeal homologs of bacterial type IV pilus biosynthesis components has revealed important aspects of flagellum and pilus biosynthesis and the mechanisms regulating motility and adhesion in archaea, many questions remain. Therefore, we screened a Haloferax volcanii transposon insertion library for motility mutants using motility plates and adhesion mutants, using an adapted air–liquid interface assay. Here, we identify 20 genes, previously unknown to affect motility or adhesion. These genes include potential novel regulatory genes that will help to unravel the mechanisms underpinning these processes. Both screens also identified distinct insertions within the genomic region lying between two chemotaxis genes, suggesting that chemotaxis not only plays a role in archaeal motility, but also in adhesion. Studying these genes, as well as hypothetical genes hvo_2512 and hvo_2876—also critical for both motility and adhesion—will likely elucidate how these two systems interact. Furthermore, this study underscores the usefulness of the transposon library to screen other archaeal cellular processes for specific phenotypic defects.

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Section: Resultsmentioning

confidence: 99%

Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants

Legerme

Yang

Esquivel

et al. 2016

Life

View full text Add to dashboard Cite

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“…Due to these factors, targeting a biofilm to prevent mycobacterial growth and spread continues to be challenging. Luk and co‐workers showed that maltose‐containing alkyl glycosides restrained bacterial adhesion and biofilm formation in P. aeruginosa . Our earlier studies demonstrated that synthetic glycolipids not only inhibited mycobacterial growth, but also impeded biofilm formation and sliding motility .…”

Section: Resultsmentioning

confidence: 75%

“…Synthetic glycolipids composed of the GalNAcβ(1–4)Galβ disaccharide moiety have emerged as a potent inhibitor of surface adhesion and biofilm formation in Pseudomonas aeruginosa . Studies also reveal that glycolipids with maltose moieties modulate quorum sensing and inhibit adhesion and biofilm formation of P. aeruginosa . Concentration‐dependent biofilm regulation was observed in a non‐rhamnolipid‐producing mutant strain of P. aeruginosa by synthetic rhamnolipids …”

Section: Introductionmentioning

confidence: 99%

Mannopyranoside Glycolipids Inhibit Mycobacterial and Biofilm Growth and Potentiate Isoniazid Inhibition Activities in M. smegmatis

et al. 2019

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Lipomannan and lipoarabinomannan are integral components of the mycobacterial cell wall. Earlier studies demonstrated that synthetic arabinan and arabinomannan glycolipids acted as inhibitors of mycobacterial growth, in addition to exhibiting inhibitory activities of mycobacterial biofilm. Herein, it is demonstrated that synthetic mannan glycolipids are better inhibitors of mycobacterial growth, whereas lipoarabinomannan has a higher inhibition efficiency to biofilm. Syntheses of mannan glycolipids with a graded number of mannan moieties and an arabinomannan glycolipid are conducted by chemical methods and subsequent mycobacterial growth and biofilm inhibition studies are conducted on Mycobacterium smegmatis. Growth inhibition of (73±3) % is observed with a mannose trisaccharide containing a glycolipid, whereas this glycolipid did not promote biofilm inhibition activity better than that of arabinomannan glycolipid. The antibiotic supplementation activities of glycolipids on growth and biofilm inhibitions are evaluated. Increases in growth and biofilm inhibitions are observed if the antibiotic is supplemented with glycolipids, which leads to a significant reduction of inhibition concentrations of the antibiotic.

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“…Luk and co‐workers demonstrated that maltose‐containing alkyl glycosides inhibit bacterial adhesion and biofilm in the case of P . aeruginosa . Our earlier studies on arabinomannan pentasaccharide glycolipids found that they inhibited M .…”

Section: Resultsmentioning

confidence: 99%

“…Maltose‐containing glycolipids were demonstrated by Luk and co‐workers to modulate quorum‐sensing circuits, leading to activation of swarming motility in a non‐swarming mutant of P. aeruginosa , even when the glycolipids inhibited the adhesion and biofilm formation . Similarly, the same authors recently identified that synthetic rhamnolipids could either promote or inhibit biofilm formation in a non‐rhamnolipid‐producing mutant strain of P. aeruginosa , depending on the concentrations of the synthetic rhamnolipids .…”

Section: Introductionmentioning

confidence: 99%

Synthetic Arabinomannan Heptasaccharide Glycolipids Inhibit Biofilm Growth and Augment Isoniazid Effects in Mycobacterium smegmatis

et al. 2017

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Biofilm formation, involving attachment to an adherent surface, is a critical survival strategy of mycobacterial colonies in hostile environmental conditions. Here we report the synthesis of heptasaccharide glycolipids based on mannopyranoside units anchored on to a branched arabinofuranoside core. Two types of glycolipids-2,3-branched and 2,5-branched-were synthesized and evaluated for their efficacies in inhibiting biofilm growth by the non-pathogenic mycobacterium variant Mycobacterium smegmatis. Biofilm formation was inhibited at a minimum biofilm growth inhibition concentration (MBIC) of 100 μg mL in the case of the 2,5-branched heptasaccharide glycolipid. Further, we were able to ascertain that a combination of the drug isoniazid with the branched heptasaccharide glycolipid (50 μg mL ) potentiates the drug, making it three times more effective, with an improved MBIC of 30 μg mL . These studies establish that synthetic glycolipids not only act as inhibitors of biofilm growth, but also provide a synergistic effect when combined with significantly lowered concentrations of isoniazid to disrupt the biofilm structures of the mycobacteria.

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Specific Maltose Derivatives Modulate the Swarming Motility of Nonswarming Mutant and Inhibit Bacterial Adhesion and Biofilm Formation by Pseudomonas aeruginosa

Cited by 18 publications

References 62 publications

Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants

Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants

Mannopyranoside Glycolipids Inhibit Mycobacterial and Biofilm Growth and Potentiate Isoniazid Inhibition Activities in M. smegmatis

Synthetic Arabinomannan Heptasaccharide Glycolipids Inhibit Biofilm Growth and Augment Isoniazid Effects in Mycobacterium smegmatis

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