Specific Lymphocyte Stimulation by Purified, Heat-inactivated Hepatitis-B Antigen

Gast, G. C. De; Houwen, B; Nieweg, H. O.

doi:10.1136/bmj.4.5894.707

Cited by 34 publications

(9 citation statements)

References 12 publications

(9 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The finding of no or weak lymphocyte sensitization to HBsAg in patients with chronic (18) or acute (19) type B hepatitis has been described. Many other hepatitis B virus (HBV)-correlated antigens or auto-antigens might play a role in stimulating the release of lymphokines and they could do it even starting from an increased basal level.…”

Section: Discussionmentioning

confidence: 99%

Immune mechanisms for hepatic fibrogenesis. T‐lymphocyte‐mediated stimulation of fibroblast collagen production in chronic active hepatitis

Casini

Ricci

Paoletti

et al. 1985

Liver

View full text Add to dashboard Cite

ABSTRACT— Lymphocytes can produce soluble factors capable of enhancing fibroblast proliferation and collagen synthesis. T‐lymphocytes, adherent cells and undifferentiated peripheral blood mononuclear cells from patients with HBsAg‐positive chronic active hepatitis and from HBsAg healthy carriers were triggered with purified HBsAg and tested for their ability to enhance collagen production by human dermal fibroblast cultures. Purified HBsAg did not induce any proliferative response in the mononuclear cell cultures. Addition of patient T‐lymphocyte supernates to monolayers of fibroblast consistently resulted in a significant enhancement of collagen production. On the contrary, supernates harvested from adherent cell cultures did not demonstrate any stimulatory activity. We therefore assumed that the observed enhancement of collagen production was probably the result of lymphokine(s) produced by T‐lymphocytes. This fibrogenic factor (or factors), which is released by T‐cells independently of the presence in vitro of HBsAg, is stable at –80°C, not dialyzable and, by Sephadex G‐100 gel filtration, is present in a fraction which collects substances of a molecular weight between 50000 and 100000. Mononuclear cell supernates from HBsAg healthy carriers did not influence fibroblast collagen accumulation. These data emphasize the possible role that lymphokines may play in the pathogenesis of fibrosis during the natural history of chronic liver disease.

show abstract

Section: Discussionmentioning

confidence: 99%

Immune mechanisms for hepatic fibrogenesis. T‐lymphocyte‐mediated stimulation of fibroblast collagen production in chronic active hepatitis

Casini

Ricci

Paoletti

et al. 1985

Liver

View full text Add to dashboard Cite

show abstract

“…Especially the T cell response to hepatitis B vaccine has not been fully characterized. Several studies were performed to detect the lymphocyte proliferative response in vitro with purified HBsAg, recombinant HBsAg, preS1, preS2 and peptides of HBsAg, but results were not consistent [26][27][28][29] . The immune response to HBsAg in vitro was difficult to detect and might not be detected in all vaccinated individuals [27] .…”

Section: Discussionmentioning

confidence: 99%

“…A correlation between in vivo antibody production and in vitro T cell proliferative response following immunization with HBsAg vaccine has been reported [24,25] . In previous studies we demonstrated that the B cell memory to HBsAg persisted for a long time after HB vaccination [26] . The purpose of this study was to determine whether the HBsAg-specific T lymphocyte memory could persist for a long time after HB vaccination especially in vaccine recipients whose serum anti-HBs level was less than 10 IU/L in an attempt to determine the optimal policy of booster vaccination.…”

Section: Introductionmentioning

confidence: 99%

Long term persistence of T cell memory to HBsAg after hepatitis B vaccination

Wang¹,

Boland²,

Hattum³

et al. 2004

WJG

View full text Add to dashboard Cite

AIM:To determine if the T cell memory to HBsAg can persist for a long time after hepatitis B (HB) vaccination. METHODS:Thirty one vaccine recipients who were healthcare workers (18 females and 13 males aged 34-58 years) from Utrecht University Hospital, Netherlands, and had previously received a standard course of vaccination for hepatitis B were investigated and another 9 unvaccinated healthy volunteers from the same hospital were used as the control. Blood samples were taken just before the experiment to test serum anti-HBs levels and the subjects were classified into different groups according to their serum titers of antiHBs and vaccination history. Their peripheral blood mononuclear cells (PBMC) were isolated from freshly heparinized venous blood and the proliferative response of T lymphocytes to the recombinant hepatitis B surface antigen (HBsAg) was investigated. RESULTS:Positive serum anti-HBs was found in 61.3% (19/31) vaccine recipients and a significant in vitro lymphocyte proliferative response to recombinant HBsAg was observed in all the vaccinees with positive anti-HBs. Serum anti-HBs level 10 IU/L was found in 38.7% (12/31) subjects. In this study, we specially focused on lymphocyte proliferative response to recombinant HBsAg in those vaccine recipients with serum anti-HBsAg less than 10 IU/L. Most of them had received a standard course of vaccination about 10 years before. T lymphocyte proliferative response was found positive in 7 of the 12 vaccine recipients. These results confirmed that HBsAg-specific memory T cells remained detectable in the circulation for a long time after vaccination, even when serum anti-HBs level had been undetectable. CONCLUSION:The T cell memory to HBsAg can persist for at least 10 years after HB vaccination. Further booster injection is not necessary in healthy responders to HB vaccine.Wang RX, Boland GJ, van Hattum J, de Gast GC. Long term persistence of T cell memory to HBsAg after hepatitis B vaccination.

show abstract

“…Lymphocyte reactivity to the antigens was expressed as the difference in dpm between the cultures with antigen and the control cultures without antigen. A difference of more than 400 dpm was considered evidence of re- No. 4 LYMPHOCYTE REACTIVITY IN MALIGNANT MELANOMA de G a t et al…”

Section: Lymphocyte Culturesmentioning

confidence: 99%

“…Lymphocyte reactivity to PHA in melanoma patients has been reported to be normal in all stages.m We had similar findings, with the exception of Stage IIIC, but this stage was not regarded separately in the above-mentioned report. Another author1 has found a lowered lymphocyte reactivity to PHA in patients with No. 4 LYMPHOCYTE REACTIVITY IN MALIGNANT MELANOMAde Gast et al 1297…”

mentioning

confidence: 96%

Humoral and cell-mediated immune response in patients with malignant melanoma.I. In vitro lymphocyte reactivity to pha and antigens following immunization

Gast

Koops

Huiges

et al. 1975

Cancer

Self Cite

View full text Add to dashboard Cite

In vitro lymphocyte function was evaluated in 61 patients with different clinical stages of malignant melanoma. Thirty‐one of these patients had localized disease, 13 regional metastases, 10 distant lymph node or skin metastases, and 7 visceral metastases. Following immunization, in vitro lymphocyte reactivity to three antigens (diphtheria toxoid, tetanus toxoid and α‐hemocyanin of Helix pomatia) was studied in the presence of autologous serum, in addition to lymphocyte reactivity to phytohemagglutinin (PHA). The relationship of these tests with the clinical stage and the subsequent course of the disease in a 6 months' observation period was determined. The patients with visceral metastases (7) had a lowered lymphocyte reactivity to PHA compared with controls and the patients with other stages, while they also had a low reactivity to the test antigens (only significantly lowered compared with patients with localized disease). All these patients showed tumor progression or died from metastatic disease. Between the other stages (54 patients) there was no difference in lymphocyte reactivity to the test antigens or PHA. No correlation between lymphocyte reactivity to PHA and the subsequent course of the disease could be demonstrated in these 54 patients. However, lymphocyte reactivity to the test antigens following immunization showed a definite correlation with the subsequent course. Sixty‐four percent (9/14) of patients without any lymphocyte reactivity to the three antigens showed tumor recurrence or progression, against 3% (1/40) of patients with positive lymphocyte reactivity to one, two, or three antigens. A suppressive effect of autologous serum on lymphocyte reactivity could be found only in 1 of 20 patients with a low reactivity to PHA or antigens. It is concluded that defects in lymphocyte function are related to subsequent tumor growth in patients with malignant melanoma.

show abstract

Specific Lymphocyte Stimulation by Purified, Heat-inactivated Hepatitis-B Antigen

Cited by 34 publications

References 12 publications

Immune mechanisms for hepatic fibrogenesis. T‐lymphocyte‐mediated stimulation of fibroblast collagen production in chronic active hepatitis

Immune mechanisms for hepatic fibrogenesis. T‐lymphocyte‐mediated stimulation of fibroblast collagen production in chronic active hepatitis

Long term persistence of T cell memory to HBsAg after hepatitis B vaccination

Humoral and cell-mediated immune response in patients with malignant melanoma.I. In vitro lymphocyte reactivity to pha and antigens following immunization

Contact Info

Product

Resources

About