Specific localization of transcription factors in the chromatin of mouse mature spermatozoa

Pittoggi, Carmine; Magnano, Anna Rosa; Sciamanna, Ilaria; Giordano, Roberto; Lorenzini, R; Spadafora, Corrado

doi:10.1002/mrd.1066

Cited by 34 publications

(25 citation statements)

References 43 publications

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“…For example, TBP was not detected in mature sperms by the immunochemical method (Martianov et al, 2002a and this study); however, an immunoprecipitation assay demonstrated that the TBP-binding sites in sperm appeared to be occupied. In addition, with the use of appropriate antibody and protocol, Oct-1, Oct-4, Ets-1, and C/EBP were detected in the sperm nucleus (Pittoggi et al, 2001). In the present study, some CFs, e.g., TFIIB, SRG3, and HDAC1 were not detected in the immunochemical assay but detected in Western blots.…”

Section: Discussioncontrasting

confidence: 51%

Erasure of the paternal transcription program during spermiogenesis: The first step in the reprogramming of sperm chromatin for zygotic development

Zheng

Xia

Ding

et al. 2008

Developmental Dynamics

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Male germ cells possess a unique epigenetic program and express a male-specific transcription profile. However, when its chromatin is passed onto the zygote, it expresses an transcription/epigenetic program characteristic of the zygote. The mechanism underlying this reprogramming process is not understood at present. In this study, we show that an extensive range of chromatin factors (CFs), including essential transcription factors and regulators, remodeling factors, histone deacetylases, heterochromatin-binding proteins, and topoisomerases, were removed from chromatin during spermiogenesis. This process will erase the paternal epigenetic program to generate a relatively naive chromatin, which is likely to be essential for installation of the zygotic developmental program after fertilization. We have also showed that transcription termination in male germ cells was temporally correlated with CF dissociation. A genome-wide CF dissociation will inevitably disassemble the transcription apparatus and regulatory mechanism and lead to transcription silence. Based on data presented in this and previous studies (Sun et al., Cell Research [2007] 17:117-134), we propose that paternal-zygotic transcription reprogramming begins with a genome-wide CF dissociation to erase the existing transcription program in later stages of spermatogenesis. This will be followed by assembling of the zygotic equivalent after fertilization. The transcription/epigenetic program of the male germ cell is transformed into a zygotic one using an erase-and-rebuild strategy similar to that used in the maternal-zygotic transition. It is also noted that transcription is terminated long after meiosis is completed and before chromatin becomes highly condensed during spermatogenesis. The temporal order of these events suggests that transcription silence does not have to be coupled to meiosis or chromatin condensation.

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Section: Discussioncontrasting

confidence: 51%

Erasure of the paternal transcription program during spermiogenesis: The first step in the reprogramming of sperm chromatin for zygotic development

Zheng

Xia

Ding

et al. 2008

Developmental Dynamics

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“…The spermatozoon is a highly differentiated and transcriptionally silent cell; nevertheless, it does contain some transcription factors (Herrada & Wolgemuth 1997, D'Cruz et al 2001, Pittoggi et al 2001. Among them, Stat1 was reported in human spermatozoa, whereas Stat4 was reported in both human and mouse spermatozoa; mouse Stat4 was located in the sperm PT (Herrada & Wolgemuth 1997, D'Cruz et al 2001.…”

Section: Discussionmentioning

confidence: 99%

“…Even though the spermatozoon is considered as a transcriptionally silent cell, it does contain some transcription factors (Herrada & Wolgemuth 1997, D'Cruz et al 2001, Pittoggi et al 2001. In spermatozoa, the Jak-Stat members detected to date are tyrosine kinase 2 (Tyk2), Stat4 andStat1 (Herrada &Wolgemuth 1997, D'Cruz et al 2001).…”

Section: Introductionmentioning

confidence: 99%

In spermatozoa, Stat1 is activated during capacitation and the acrosomal reaction

Bastián

Zepeda‐Bastida²,

Uribe³

et al. 2007

Reproduction

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A role for sperm-specific proteins during the early embryonic development has been suggested by a number of recent studies. However, little is known about the participation of transcription factors in that stage. Here, we show that the signal transducer and activator of transcription 1 (Stat1), but not Stat4, was phosphorylated in response to capacitation and the acrosomal reaction (AR). Moreover, Stat1 phosphorylation correlated with changes in its localization: during capacitation, Stat1 moved from the cytoplasm to the theca/flagellum fraction. During AR, Stat1 phosphorylation increased again. In addition, blocking protein kinase A (PKA) and PKC during capacitation abolished both phosphorylation and migration of Stat1. Our results show tight spatio-temporal rearrangements of Stat1, suggesting that after fertilization Stat1 participates in the first rounds of transcription within the male pronucleus.

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“…The finding that ␤-arrestin2 mediates transactivation upon hOR17-4 stimulation suggests participation in nuclear functions in mammalian spermatozoa, despite the fact that these cells have a highly condensed, inactive chromatin structure. Interestingly, a growing number of studies indicate that the nucleus of mature spermatozoa contains potentially active chromatin domains (GardinerGarden et al, 1998;Pittoggi et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Novel function of β-arrestin2 in the nucleus of mature spermatozoa

Neuhaus

Mashukova

Barbour

et al. 2006

Journal of Cell Science

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A growing number of proteins originally found in endocytic structures of the plasma membrane appear to be able to traffic into the nucleus, but the cellular function of this translocation remains unclear. We have found that β-arrestin2, which typically shows a cytoplasmic localization owing to constitutive nuclear export, appears in the nucleus after stimulation of the G-protein-coupled odorant receptor hOR17-4. In the nucleus, β-arrestin2 was involved in transcriptional regulation as shown by a Gal4-based transactivation assay. Moreover, we discovered that β-arrestin2 and hOR17-4, a receptor known to have a role in sperm-egg communication, colocalize in the midpiece of mature human spermatozoa. Stimulation of hOR17-4 in spermatozoa induced PKA-dependent translocation of β-arrestin2 to the nucleus and nuclear accumulation of phosphorylated MAPKs. Analysis of the interaction partners of β-arrestin2 indicates that odorant receptor signaling in spermatozoa may be important for the regulation of gene expression during the early processes of fertilization.

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Specific localization of transcription factors in the chromatin of mouse mature spermatozoa

Cited by 34 publications

References 43 publications

Erasure of the paternal transcription program during spermiogenesis: The first step in the reprogramming of sperm chromatin for zygotic development

Erasure of the paternal transcription program during spermiogenesis: The first step in the reprogramming of sperm chromatin for zygotic development

In spermatozoa, Stat1 is activated during capacitation and the acrosomal reaction

Novel function of β-arrestin2 in the nucleus of mature spermatozoa

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