Specific labelling of cell populations in blood with targeted immuno-fluorescent/magnetic glyconanoparticles

Gallo, Juan; Garcı́a, Isabel; Genicio, Nuria; Padró, Daniel; Penadés, Soledad

doi:10.1016/j.biomaterials.2011.09.010

Cited by 33 publications

(34 citation statements)

References 44 publications

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“…For instance, by incorporating a fluorescent dye into gold-coated MNPs, Gallo et al [29] designed dual contrast agents that could be used to assess cellular labelling in blood. For the specific labelling of lowpopulation peripheral blood mononuclear cells, glycoMNPs were functionalized with protein G in order to further incorporate in an oriented manner an anti-CD45 antibody to target leucocytes.…”

Section: Cell Targetingmentioning

confidence: 99%

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Recent advances in biosensing using magnetic glyconanoparticles

2015

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In this critical review we discuss the most recent advances in the field of biosensing applications of magnetic glyconanoparticles. We first give an overview of the main synthetic routes to obtain magnetic-nanoparticle-carbohydrate conjugates and then we highlight their most promising applications for magnetic relaxation switching sensing, cell and pathogen detection, cell targeting and magnetic resonance imaging. We end with a critical perspective of the field, identifying the main challenges to be overcome, but also the areas where the most promising developments are likely to happen in the coming decades.

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Section: Cell Targetingmentioning

confidence: 99%

“…3). The carboxyl groups available on the surface of the glycoMNPs can be used as anchoring points for further functionalization to yield (immuno)fluorescent glycoMNPs [28][29][30][31][32].…”

Section: Ligand Exchangementioning

confidence: 99%

Recent advances in biosensing using magnetic glyconanoparticles

2015

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“…Another recent example was the preparation of glycoferrite nanoparticles (superparamagnetic water-soluble coreshell X Fe 2 O 4 (where X = iron (Fe), manganese (Mn), or cobalt (Co)) nanoparticles) with different carbohydrates and carboxylic linkers and functionalized with a fluorescent dye (Texas Red). Additionally, protein G was conjugated to attach well oriented antibodies to obtain immuno-fluorescent-magnetic glycoferrites [97,98]. These multimodal GNPs have allowed for the specific labeling of peripheral blood mononuclear cells (PBMCs) present in blood in a percentage below 2% by fluorescent microscopy and by magnetic resonance imaging [97].…”

Section: Metallic Glyconanoparticlesmentioning

confidence: 99%

“…Additionally, protein G was conjugated to attach well oriented antibodies to obtain immuno-fluorescent-magnetic glycoferrites [97,98]. These multimodal GNPs have allowed for the specific labeling of peripheral blood mononuclear cells (PBMCs) present in blood in a percentage below 2% by fluorescent microscopy and by magnetic resonance imaging [97]. The study of their uptake, the intracellular fate by confocal microscopy [99], and the in vivo tracking of the migration of endogenous neural progenitors in response to an induced tumor have been carried out by MRI.…”

Section: Metallic Glyconanoparticlesmentioning

confidence: 99%