Specific Isolation of Phenolic Lipids By Phase Separations

Resorcinolic Lipids, the Natural Non-isoprenoid Phenolic Amphiphiles and Their Biological Activity

“…In some studies notably with Ononis species 70-72,74,75 the array of compounds reported appears partly attributable to methylation or acetylation reactions carried out during column chromatographic separation to enhance separations. An interesting approach for the prepurification and selective separation of resorcinolic lipid from phenolic lipids or resorcinolic lipids from impurities has been recently reported in which a selective partitioning of different non-isoprenoic phenolic lipids between two immiscible organic solvents (e.g., diol and hexane) is used. , Column and thin-layer chromatographies on unmodified or hydrophobic (reversed-phase) silica gels are used depending on preparative/analytical objectives. In general, chromatography on plain silica gel is used for the isolation and purification of the resorcinolic lipid fraction, whereas partition chromatography on hydrophobic silica gel (silica gel modified with octadecylsilane residues, RP-18, is most frequently used) is applied for the separation of individual homologues.…”

Phenolic Structure and Colour in Mannich Reaction Products

“…In Mannich reactions, cardol was more reactive than cardanol and this was used subsequently in two methods for the preparation of purer cardanol with a reduced % cardol 23,24 although a phase separation procedure was later 25 found to be more efficient. The patentees 1 do not mention the formation of a red by-product although their raw material is described as 90-92% cardanol and 2-10% of cardol.…”