Specific inulin‐type fructan fibers protect against autoimmune diabetes by modulating gut immunity, barrier function, and microbiota homeostasis

Chen, Kang; Chen, Hao; Faas, Marijke M.; Haan, Bart J. de; Li, Jiahong; Xiao, Peipei; Zhang, Hao; Diana, James S.; Vos, Paul de; Sun, Jia

doi:10.1002/mnfr.201601006

Cited by 123 publications

(88 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This may be a possible mechanism for the observed enhancing effects of hMOs and NDCs and its beneficial effects on gut microbiota that need the glycocalyx to adhere to the gut epithelium, with subsequent positive effects on fermentation products short chain fatty acids (SCFAs) and vitamins . Also, it may be a possible mechanistic explanation for the hMOs and NDCs induced enhanced gut barrier function . As shown in our current study the glycocalyx reinforcing effects of the tested food ingredients are due to direct effects on the epithelial cells and not induced by possible microbiota effects as all tests were performed in the absence of bacteria.…”

Section: Discussionmentioning

confidence: 50%

Modulation of Intestinal Epithelial Glycocalyx Development by Human Milk Oligosaccharides and Non‐Digestible Carbohydrates

Kong

Elderman

Cheng

et al. 2019

Molecular Nutrition Food Res

View full text Add to dashboard Cite

Scope The epithelial glycocalyx development is of great importance for microbial colonization. Human milk oligosaccharides (hMOs) and non‐digestible carbohydrates (NDCs) may modulate glycocalyx development. Methods and results The effects of hMOs and NDCs on human gut epithelial cells (Caco2) are investigated by quantifying thickness and area coverage of adsorbed albumin, heparan sulfate (HS), and hyaluronic acid (HA) in the glycocalyx. Effects of hMOs (2′‐FL and 3‐FL) and NDCs [inulins with degrees of polymerization (DP) (DP3‐DP10, DP10‐DP60, DP30‐DP60) and pectins with degrees of methylation (DM) (DM7, DM55, DM69)] are tested using immunofluorescence staining at 1 and 5 days stimulation. HMOs show a significant enhancing effect on glycocalyx development but effects are structure‐dependent. 3‐FL induces a stronger albumin adsorption and increases HS and HA stronger than 2′‐FL. The DP3‐DP10, DP30‐60 inulins also increase glycocalyx development in a structure‐dependent manner as DP3‐DP10 selectively increases HS, while DP30‐DP60 specifically increases HA. Pectins have less effects, and only increase albumin adsorption. Conclusion Here, it is shown that 2′‐FL and 3‐FL and inulins stimulate glycocalyx development in a structure‐dependent fashion. This may contribute to formulation of effective hMO and NDC formulations in infant formulas to support microbial colonization and gut barrier function.

show abstract

Section: Discussionmentioning

confidence: 50%

Modulation of Intestinal Epithelial Glycocalyx Development by Human Milk Oligosaccharides and Non‐Digestible Carbohydrates

Kong

Elderman

Cheng

et al. 2019

Molecular Nutrition Food Res

View full text Add to dashboard Cite

show abstract

“…Interestingly, we observed that those pigs fed IN, WB and MIX had a low concentration of acetic acid and total SCFAs in colon. The action of wheat bran fibre depends on the composition arabinoxylan of wheat bran and its particle size, and the different effects of inulin on energy metabolism are related with its various type and chain length (Chen et al., 2015; Chen et al, 2017; Koo, Hossain, & Nyachoti, 2017; Molist, Manzanilla, Pérez, & Nyachoti, 2012; Molist et al., 2009). Chen et al.…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary fibres on gut microbial metabolites and liver lipid metabolism in growing pigs

Chen

et al. 2020

Animal Physiology Nutrition

View full text Add to dashboard Cite

This study was conducted to investigate the effects of dietary supplementation with wheat bran fibre, inulin and their combination on growth performance, short‐chain fatty acids (SCFAs) production in caecum and colon and liver lipid metabolism in growing pigs. A total of 48 Duroc × Landrace × Yorkshire cross‐bred growing pigs (73 ± 2 days of age; 24.37 ± 2.86 kg) were allocated to four groups randomly, each group consisting of six pens with two pigs each. The pigs were fed a control diet (CON), a diet containing 2% wheat bran fibre (WB), a diet containing 2% inulin (IN), and a diet containing both of 1% wheat bran fibre and 1% inulin (MIX), respectively. The trial lasted for 28 days. The results showed that MIX fed pigs had a higher percentage of fat in the liver than those fed the CON (p < .05). IN, WB or MIX feeding decreased the concentrations of acetate and total SCFAs in colon compared with CON (p < .01). Feeding WB or IN also decreased the colonic butyrate concentrations compared with CON (p < .01). However, the serum level of valeric acid was elevated in the IN, WB and MIX group (p < .01). MIX fed pigs tended to have lower levels of propionate in serum than the WB fed pigs (p = .098). MIX feeding enhanced the mRNA expression of lipid synthesis‐related genes in liver compared with CON (p < .05). Feeding IN decreased the expression of bile acids synthesis‐related genes in liver and increased mRNA expression of SCFAs transporter SLC16A1 in colon compared with CON (p < .05). In this study, these data indicated that the combined supplementation of wheat bran fibre and inulin decreased the SCFAs concentrations in the colon, enhanced the genes FAS and HNF‐4α mRNA expression in liver and induced liver lipid accumulation in growing pigs.

show abstract

“…By tuning down excessive innate responses, promotion of tissue‐protective mechanisms and stimulating specific adaptive immunity, fermentable inulin fiber, and SCFAs create an immune equilibrium that protects against immune‐associated pathologies (Trompette et al., ). Long‐chain inulin‐type fructan fiber protects against autoimmune disease by modulating microbiota homeostasis and gut immunity (K. Chen et al., ). Moreover, fermentable DF (pectin) can shape the immunological environment in the lung and suppress allergic airway disease in mice (Trompette et al., ).…”

Section: Effects Of Dfs From Fruits and Vegetables On Host Healthmentioning

confidence: 99%

Dietary Fibers from Fruits and Vegetables and Their Health Benefits via Modulation of Gut Microbiota

Cui

Lian²,

Zhao

et al. 2019

Comp Rev Food Sci Food Safe

131

View full text Add to dashboard Cite

Dietary fibers (DFs) regulate host health through various mechanisms related to their dietary sources, specific physicochemical structures, fermentability, and physiological properties in the gut. Considering the numerous types and sources of DFs and their different physicochemical and physiological properties, it is challenging yet important to establish the key mechanisms for the beneficial health effects of DFs. In this review, the types and structures of DFs from different fruits and vegetables were summarized and the effects of different processing methods on DF properties were discussed. Moreover, the impacts of DFs on gut microbial ecology, host physiology, and health were described. Understanding the complex interaction between different DFs and gut microbiota is vital for personalized nutrition. It is also important to comprehend factors influencing gut microbiota and strategies to regulate the microbiota, thereby augmenting beneficial health responses. The exploration of molecular mechanism linking DFs, gut microbiota, and host physiology may allow for the identification of effective targets to fight against major chronic diseases.

show abstract

Specific inulin‐type fructan fibers protect against autoimmune diabetes by modulating gut immunity, barrier function, and microbiota homeostasis

Abstract: Our data demonstrate that ITF(l) but not ITF(s) delays the development of T1D via modulation of gut-pancreatic immunity, barrier function, and microbiota homeostasis.

Cited by 123 publications

References 43 publications

Modulation of Intestinal Epithelial Glycocalyx Development by Human Milk Oligosaccharides and Non‐Digestible Carbohydrates

Modulation of Intestinal Epithelial Glycocalyx Development by Human Milk Oligosaccharides and Non‐Digestible Carbohydrates

Effects of dietary fibres on gut microbial metabolites and liver lipid metabolism in growing pigs

Dietary Fibers from Fruits and Vegetables and Their Health Benefits via Modulation of Gut Microbiota

Contact Info

Product

Resources

About