Specific interactions of liposomes with PMN leukocytes upon incorporating tuftsin in their bilayers

Singhal, Arun; Bali, Anu; Jain, Rakesh; Gupta, C. M.

doi:10.1016/0014-5793(84)81251-x

Cited by 33 publications

(10 citation statements)

References 20 publications

(6 reference statements)

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“…The tube was dried to remove any trace of solvent. The lipid film was then hydrated and dispersed in a solution of appropriate quantity of p-tuftsin (7-8% by PC weight) in Tris-buffered saline (10 mM Tris containing 150 mM NaCl, pH 7.4) and processed by probe sonication (W-220) to get the desired preparation of liposomal tuftsin (Singhal et al 1984).…”

Section: Palmitoyl Tuftsinmentioning

confidence: 99%

Augmentation of antileishmanial efficacy of miltefosine in combination with tuftsin against experimental visceral leishmaniasis

et al. 2012

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Current drugs for the treatment of visceral leishmaniasis are inadequate, and their efficacies are also compromised due to suppression of immune function during the course of infection. Miltefosine is the only promising orally active antileishmanial drug, but due to its long half-life, there is risk of development of resistance. To overcome these problems, efforts are needed to develop combination therapy of miltefosine with effective immunostimulating agents where a decrease of parasitic burden and simultaneous enhancement of adaptive immunity can be achieved. In the present study, we have explored the antileishmanial efficacy of a subcurative dose of miltefosine in combination with free as well as liposomal palmitoyl tuftsin (p-tuftsin) using a Leishmania donovani/BALB/c mouse model. When miltefosine (2.5 mg/kg for 5 days) was given with free p-tuftsin, the inhibitory effect was significantly increased from 49.6% to 66% (P < 0.01), which was further enhanced up to 81% (P < 0.001) when given after liposomal encapsulation of p-tuftsin. Significant enhancement in parasitic inhibition (93%, P < 0.01) was witnessed when animals were co-administered with liposomal p-tuftsin + 5 mg/kg × 5 days dose of miltefosine (72.1%). Enhancement in the production of Th1 cytokines (IL-12, TNF-α, and IFN-γ), reactive oxygen, and nitrogen metabolites was witnessed in the combination group. A remarkable increase in phagocytosis index was also observed indicating overall immunological enhancement to antileishmanial activity of miltefosine by p-tuftsin.

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Section: Palmitoyl Tuftsinmentioning

confidence: 99%

Augmentation of antileishmanial efficacy of miltefosine in combination with tuftsin against experimental visceral leishmaniasis

et al. 2012

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“…Egg phosphatidylcholine was isolated and purified by published procedures (18). Tuftsin modified by attachment of a palmitoyl residue to its C terminus through an ethylenediamine spacer arm (Thr-Lys-Pro-Arg-NH-(CH2)2-NH-CO-C15H31) was prepared as described previously (17).…”

Section: Materuils and Methodsmentioning

confidence: 99%

“…The RFP A334 was found to remain unaffected by the presence of liposomes and detergent and was linear up to 100 ,ug of RFP. The amount of tuftsin incorporated in liposomes was estimated as described previously (17). The incorporation efficiency was found to be about 98%.…”

Section: Materuils and Methodsmentioning

confidence: 99%

“…The peptide is known to bind specifically to macrophages/monocytes and also to potentiate the natural killer activity of these cells (8). Our earlier studies have shown that incorporation of this tetrapeptide in liposome bilayers, after its modification at the C terminus (17), increases not only its macrophage-activating property (16) but also liposome uptake by macrophages (17). The liposomes thus formed have been shown to be quite effective as drug vehicles in treatment of leishmaniasis (6) and aspergillosis (12).…”

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confidence: 99%

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Tuftsin-bearing liposomes as rifampin vehicles in treatment of tuberculosis in mice

Agarwal

Kandpal

Gupta

et al. 1994

Antimicrob Agents Chemother

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The antitubercular activity of rifampin was considerably increased when it was encapsulated in egg phosphatidylcholine liposomes. A further increase in the activity was observed when the macrophage activator tetrapeptide tuftsin was grafted on the surface of the drug-loaded liposomes. Intermittent treatments (twice weekly) with these preparations were significantly more effective than the continuous treatments. Rifampin delivered twice weekly for 2 weeks in tuftsin-bearing liposomes was at least 2,000 times more effective than the free drug in lowering the load of lung bacilli in infected animals. However, pretreatment with drug-free tuftsin-bearing liposomes did not render the pretreated animals resistant to the Mycobacterium tuberculosis infections, neither did it appreciably increase the chemotherapeutic efficacy of the liposomized rifampin. These results clearly demonstrate that liposome targeting to macrophages could considerably increase the antitubercular activity of liposomized drugs such as rifampin. Also, it shows that immunoprophylactic treatment with macrophage activators such as tuftsin does not afford any advantage in treatment of tuberculosis infections,

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“…Structure-function studies of this tetrapeptide indicate that its binding and subsequent activation of the mononuclear phagocyte system (MPS) is dependent upon rather strict conservation of its molecular structure. Direct attachment of a fatty acyl group to the Arg residue, without any spacer arm, leads to modified tuftsin, which does not allow formation of liposomes, presumably due to perturbation of the phospholipid polar head group packing by the bulky Arg residue (Singhal et al 1984). However, the activity is largely retained if modifications are restricted only to the C-terminus (Gottlieb et al 1982).…”

Section: Tuftsin: a Highly Effective Immunomodulatormentioning

confidence: 99%

Combating Fungal Infections

Ahmad¹,

Owais²,

Shahid³

et al. 2010

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Specific interactions of liposomes with PMN leukocytes upon incorporating tuftsin in their bilayers

Cited by 33 publications

References 20 publications

Augmentation of antileishmanial efficacy of miltefosine in combination with tuftsin against experimental visceral leishmaniasis

Augmentation of antileishmanial efficacy of miltefosine in combination with tuftsin against experimental visceral leishmaniasis

Tuftsin-bearing liposomes as rifampin vehicles in treatment of tuberculosis in mice

Combating Fungal Infections

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