Specific inhibition of the synthesis of influenza virus late proteins and stimulation of early, M2, and NS2 protein synthesis by 3-deazaadenosine

Fischer, Andreas; Müller, Karin; Scholtissek, Christoph

doi:10.1016/0042-6822(90)90517-u

Cited by 24 publications

(28 citation statements)

References 20 publications

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“…The hybridization was carried out according to the procedure of Wahl et al (1979) as modified by Fischer et al (1990). For the Northern blot analysis of ts 263 viral RNA, a corresponding PA probe of positive polarity was prepared.…”

Section: Methodsmentioning

confidence: 99%

“…The RNA segments were separated by agarose formamide gel electrophoresis and blotted onto a nylon membrane. For hybridization the [35S]UTP-labelled probes were synthesized on vectors derived from the pBluescript II-SK vector (Stratagene) using the T3 or T7 promoters, pANI contained nucleotides 341 to 1265 of the HA gene, pJU1 nucleotides 703 to 1565 of the NP gene, the pAN3 vector nucleotides 196 to 855 of the NS protein gene, pAN4 nucleotides 246 to 1024 of the MI protein gene (Fischer et al, 1990), and pPA contained the truncated PA gene.…”

Section: Methodsmentioning

confidence: 99%

“…The major regulation seems to occur at the level of the availability of the corresponding mRNA (Hay et al, 1977;Smith & Hay, 1982;Enami et al, 1985;Shapiro et al, 1987). However, there are some indications that additional regulation occurs at the level of translation (Hatada et at., 1989;Fischer et aL, 1990). So far, little is known concerning the mechanism of regulation and the viral components involved.…”

Section: Introductionmentioning

confidence: 99%

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A temperature-sensitive mutation in the acidic polymerase gene of an influenza A virus alters the regulation of viral protein synthesis

Herget

Scholtissek

1993

Journal of General Virology

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A temperature-sensitive mutation in the acidic polymerase gene of an influenza A virus alters the regulation of viral protein synthesis

Herget

Scholtissek

1993

Journal of General Virology

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“…We did not necessarily expect that 3DA-Ado would interfere with baculovirus protein synthesis, since the DNA isolated from baculovirus particles does not contain measurable amounts of methyl groups on CG pairs, which normally regulate promoter activities on DNA Tjia et al, 1979). However, during the course of our Short communication (Fischer et al, 1990). FPV proteins (indicated on the right) served as molecular mass markers in all experiments.…”

mentioning

confidence: 99%

“…Recently, we found that the methyltransferase inhibitor 3-deazaadenosine (3DA-Ado) specifically inhibited the replication of influenza viruses by retaining the mRNAs of only the late viral proteins, matrix protein M1, haemagglutinin (HA) and neuraminidase (NA), in the nuclei of the infected cells (Fischer et al, 1990;Vogel et al, 1994). This observation was of special interest, since influenza viruses are the only 'pure' RNA viruses with a clear nuclear phase, during which the synthesis of all eight viral mRNAs is started with the so-called capstealing process.…”

mentioning

confidence: 99%

Effect of methyltransferase inhibitors on the regulation of baculovirus protein synthesis

Bach

Cramer

Scholtissek

1995

Journal of General Virology

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In the presence of the methyltransferase inhibitor 3-deazaadenosine (3DA-Ado) the production of infectious Autographa californica nuclear polyhedrosis virus (AcMNPV) in tissue culture was only slightly affected, while the synthesis of very late proteins (polyhedrin and pl0) was abolished. The synthesis of the influenza virus proteins NS1 and HA, expressed under the polyhedrin promoter, was also abolished by 3DA-Ado. Furthermore, 3DA-Ado interfered with the shut-off of early and late AcMNPV proteins. Most of these results were also obtained with 5-azadeoxycytidine (5A-dCyt). In cells in which NS1 was produced abundantly, at least one specific AcMNPV protein was not synthesized. However, if the production of NS1 was inhibited by 3DA-Ado, or if HA was synthesized instead, this AcMNPV protein showed up normally.

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Nucleosides and Nucleotides

Suhadolnik¹,

Reichenbach²

2000

Kirk-Othmer Encyclopedia of Chemical Technology

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The naturally occurring nucleoside and nucleotide antibiotics exist as either the C ‐ or N ‐glycosides. They include ezomycin A 1 (C 26 H 38 N 8 O 15 S), ezomycin B 1 (C 26 H 39 N 7 O 17 S), ezomycin C 1 (C 26 H 37 N 7 O 16 S), ezomycin A 2 (C 19 H 26 N 6 O 12 ), ezomycin B 2 (C 19 H 25 N 5 O 13 ), ezomycin C 2 (C 19 H 25 N 5 O 13 ), showdomycin (C 9 H 11 NO 6 ), isoshowdomycin (C 9 H 11 NO 6 ), maleimycin (C 7 H 7 NO 3 ), oxazinomycin (C 9 H 11 NO 7 ), pyrazomycin (pyrazofurin) (C 9 H 13 N 3 O 6 ), formycin (C 10 H 13 N 5 O 4 ), formycin B (C 10 H 12 N 4 O 5 ), oxoformycin B (C 10 H 12 N 4 O 6 ). These antibiotics contain a variety of purine and pyrimidine rings. The naturally occurring nucleoside/nucleotide antibiotics, which have been isolated from bacteria, fungi, blue‐green algae, and marine sponges, have proven to be useful biochemical probes in eucaryotic, procaryotic, viral, fungal, and plant systems. The purine nucleosides inhibit protein synthesis, RNA and DNA synthesis, and methyltransferases; they have antimycoplasmal, antiviral, hypotensive, antifungal, antimycobacterial, and antitumor activities and induce sporulation. The pyrimidine nucleosides inhibit protein synthesis, virus replication, RNA and DNA synthesis, and cAMP phosphodiesterase. The imidazole nucleosides inhibit nucleic acid synthesis. The diazepin nucleosides inhibit adenosine deaminase (ADA). The indole nucleosides inhibit bacteria, yeast, fungi, and viruses. The N ‐nucleotide antibiotics include agrocin 84 (C 21 H 34 N 6 O 16 P 2 ), thuringiensin (C 22 H 30 N 5 O 19 P), phosmidosine (C 16 H 24 N 7 O 8 P), fosfadecin (C 13 H 19 N 5 O 10 P 2 ), and fosfocytocin (C 12 H 20 N 4 O 13 P 2 ). Thuringiensin, produced by B. thuringiensis , is a β‐exotoxin that exerts its toxic action on insects and mammals through the inhibition of RNA polymerases. Phosmidosine inhibits pore formation of Botrytis cinerea and Aspergillus niger . Fosfadecin and fosfocytocin inhibit gram‐positive and gram‐negative bacteria.

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Specific inhibition of the synthesis of influenza virus late proteins and stimulation of early, M2, and NS2 protein synthesis by 3-deazaadenosine

Cited by 24 publications

References 20 publications

A temperature-sensitive mutation in the acidic polymerase gene of an influenza A virus alters the regulation of viral protein synthesis

A temperature-sensitive mutation in the acidic polymerase gene of an influenza A virus alters the regulation of viral protein synthesis

Effect of methyltransferase inhibitors on the regulation of baculovirus protein synthesis

Nucleosides and Nucleotides

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