Specific immunofluorescent staining of pathogenic treponemes with a monoclonal antibody

Ito, Fumiyuki; Hunter, Elizabeth F.; George, Robert W.; Pope, Victoria; Larsen, Sandra A.

doi:10.1128/jcm.30.4.831-838.1992

Cited by 44 publications

(24 citation statements)

References 25 publications

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“…oral mucosa). Notably, IHC directed against T. pallidum improved both the sensitivity and specificity 8,9 . Immunostaining of ulcer specimens with fluorescent anti‐ T. pallidum antibodies has improved the level of sensitivity to higher than that of dark‐field examination.…”

mentioning

confidence: 99%

Comparative analysis of immunohistochemistry, polymerase chain reaction and focus-floating microscopy for the detection of Treponema pallidum in mucocutaneous lesions of primary, secondary and tertiary syphilis

Müller

Eisendle

Bräuninger

et al. 2011

British Journal of Dermatology

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FFM is a highly sensitive and specific method to detect T. pallidum in tissue from mucocutaneous syphilis lesions. Our results indicate that a combination of PCR and FFM, as the most sensitive approach, could provide an additional benefit for the histopathological diagnosis of (late) secondary and tertiary syphilis and may be helpful in cases where serological testing of T. pallidum antibodies has failed, but the clinical suspicion for syphilis remains.

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mentioning

confidence: 99%

Comparative analysis of immunohistochemistry, polymerase chain reaction and focus-floating microscopy for the detection of Treponema pallidum in mucocutaneous lesions of primary, secondary and tertiary syphilis

Müller

Eisendle

Bräuninger

et al. 2011

British Journal of Dermatology

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“…MAbs have been successfully used in immunofluorescence based diagnostic tests for Lyme and syphilis, two diseases also caused by spirochetes. (20,21) The antibodies reported here were able to attach to the bacterial outer membrane after the two fixing treatments, providing evidence of their usefulness for immunohistochemical tests.…”

Section: Discussionmentioning

confidence: 58%

Monoclonal Antibodies Against LipL32, The Major Outer Membrane Protein of PathogenicLeptospira: Production, Characterization, and Testing in Diagnostic Applications

et al. 2007

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Pathogenic serovars of Leptospira have a wide antigenic diversity attributed mainly to the lipopolysaccharide present in the outer membrane. In contrast, antigens conserved among pathogenic serovars are mainly represented by outer membrane proteins. Surface exposure of a major and highly conserved outer membrane lipoprotein (LipL32) was recently demonstrated on pathogenic Leptospira. LipL32 in its recombinant form (rLipL32) was used to immunize BALB/c mice to develop murine monoclonal antibodies (MAbs). Three MAbs against rLipL32 were produced, isotyped, and evaluated for further use in diagnostic tests of leptospirosis using different approaches. MAbs were conjugated to peroxidase and evaluated in a native protein enzyme-linked immunosorbent assay (ELISA) with intact and heat-treated leptospiral cells, conjugated to fluorescein isothiocyanate (FITC) for indirect immunofluorescence with intact and methanol fixed cells and were used for LipL32 immunoprecipitation from leptospiral cells. rLipL32 MAbs conjugated to peroxidase or used as primary antibody bound to intact and heat-treated cells in ELISA, proving that they could be used in enzyme immunoassays for detection of the native protein. In immunofluorescence assay, MAbs labeled bacterial cells either intact or methanol fixed. Two MAbs were able to immunoprecipitate the native protein from live and motile leptospiral cells and, adsorbed onto magnetic beads, captured intact bacteria from artificially contaminated human sera for detection by polymerase chain reaction (PCR) amplification. Results of this study suggest that the MAbs produced can be useful for the development of diagnostic tests based on detection of LipL32 leptospiral antigen in biological fluids.

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“…The stained sections are easy to observe and keep. Ito et al also reported a specific immunofluorescent staining technique for pathogenic treponemes using a monoclonal antibody (4).…”

Section: Discussionmentioning

confidence: 99%

A Case of Secondary Syphilis with Mucous Patches on the Hard Palate

Ban

Ohtani

Seishima

1995

The Journal of Dermatology

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A rare case of secondary syphilis showing mucous patches on the hard palate is reported. A 31-year-old male had two erosive patches which were slightly raised on his hard palate. A linear lesion was also present on the inside of his right alveolar process. Many red-brown macules on his abdomen and bilateral inguinal lymphadenopathy accompanied these symptoms. The serological tests for syphilis were positive: VDRL test 1:512, TPHA test 1:20,480, and IgM-FTA-ABS test 1:40. Immunohistochemical staining with rabbit monoclonal antibody to Treponema pallidum by the biotin-streptoavidin system detected treponemal organisms in the paraffin-embedded specimen from his mucous patch. A diagnosis of secondary syphilis was made, and he was given amoxicillin (AMPC) at 750 mg per day for 4 weeks. His eruptions, including the mucous patch, healed in a week.

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Specific immunofluorescent staining of pathogenic treponemes with a monoclonal antibody

Cited by 44 publications

References 25 publications

Comparative analysis of immunohistochemistry, polymerase chain reaction and focus-floating microscopy for the detection of Treponema pallidum in mucocutaneous lesions of primary, secondary and tertiary syphilis

Comparative analysis of immunohistochemistry, polymerase chain reaction and focus-floating microscopy for the detection of Treponema pallidum in mucocutaneous lesions of primary, secondary and tertiary syphilis

Monoclonal Antibodies Against LipL32, The Major Outer Membrane Protein of PathogenicLeptospira: Production, Characterization, and Testing in Diagnostic Applications

A Case of Secondary Syphilis with Mucous Patches on the Hard Palate

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