Specific Immobilization ofin VivoBiotinylated Bacterial Luciferase and FMN:NAD(P)H Oxidoreductase

Min, Dong Joon; Andrade, Joseph D.; Stewart, Russell J.

doi:10.1006/abio.1999.4074

Cited by 14 publications

(2 citation statements)

References 28 publications

(46 reference statements)

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“…This limitation is particularly important for rational construction of nanodevices in which the function depends on predefined stoichiometry of building blocks. Fortunately, monobiotinylation of a protein-of-interest (POI) can be achieved by molecular biological techniques, 6 and monovalent STV mutants have recently been developed. 7 Hence the control of stoichiometry can be achieved.…”

Section: Synthesis Of Dna-protein Conjugates 21 Non-covalent Couplingmentioning

confidence: 99%

Functionalization of DNA nanostructures with proteins

2011

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Proteins possess intrinsic functionalities, which have been optimized in billions of years of natural evolution. The conjugation of proteins with artificial nucleic acids allows one to further functionalize proteins with a synthetically accessible, physicochemically robust tag, which is addressable in a highly specific manner by Watson-Crick hybridization. The resulting DNA-protein conjugates can be advantageously used in a variety of applications, ranging from biomedical diagnostics to DNA-based nanofabrication. This critical review provides an overview on chemical approaches to the synthesis of DNA-protein conjugates and their applications in biomolecular nanosciences (96 references).

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Section: Synthesis Of Dna-protein Conjugates 21 Non-covalent Couplingmentioning

confidence: 99%

Functionalization of DNA nanostructures with proteins

2011

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“…5B), and the formation of the low-mobility bands with the target ODN (lane 5) showed the selective hybridization of the ODN conjugate. After the modification reactions, the intensity of the luciferase activity of the reaction mixture was reduced from 7.0610 8 to 1.0610 6 (ca. 0.1%), and its activity was stable at 48C for more than a week.…”

Section: Synthesis and Evaluation Of The Peg-modified Luciferase-odn mentioning

confidence: 99%

Synthesis and Evaluation of the Luciferase‐Oligodeoxynucleotide for the Sequence‐Selective Detection of Nucleic Acids

Nagatsugi

Nakahara

Inoué

et al. 2008

Archiv der Pharmazie

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A new method for the synthesis of ODN-luciferase conjugate was investigated as a signal-amplifying sensor of the target nucleic acids. The conjugation of the luciferase was successfully achieved between the cysteine residue and the 2-amino-6-vinylpurine nucleoside of the ODN probe without significant inactivation of luciferase. The ODN-luciferase conjugate modified with PEG retained the luciferase activity and selectivity during the hybridization with the target ODN.

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Halbsynthetische DNA‐Protein‐Konjugate für Biosensorik und Nanofabrikation

Niemeyer

2010

Angewandte Chemie

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Durch Konjugation mit artifiziellen Nucleinsäuren lassen sich Proteine mit einem leicht herstellbaren, robusten Etikett versehen. Dieses Anhängsel ist durch hochspezifische, molekulare Erkennungsmechanismen wie die Watson‐Crick‐Basenpaarung selektiv adressierbar. Solche DNA‐Protein‐Konjugate mit kombinierten Eigenschaften haben vielfältige Verwendungsmöglichkeiten, z. B. in hochempfindlichen biomedizinischen Analyseverfahren, der Grundlagenforschung zur molekularen Erkennung oder dem Aufbau von DNA‐Nanostrukturen. Dieser Aufsatz fasst die aktuellen Methoden zur Synthese dieser Konjugate zusammen und präsentiert den aktuellen Stand ihrer Anwendungen. So wurden DNA‐Protein‐Konjugate zu Modellsystemen zusammengesetzt, die die Untersuchung von katalytischen Kaskadenreaktionen oder von Lichtsammelkomplexen ermöglichen. Außerdem finden diese Hybridkonjugate Verwendung bei der Biofunktionalisierung planarer Oberflächen bei Mikro‐ und Nanoarrays oder zur Funktionalisierung anorganischer Nanopartikel. Entsprechend finden sich mögliche Anwendungen im Bereich der Detektion, der Materialwissenschaften und der Katalyse.

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Specific Immobilization ofin VivoBiotinylated Bacterial Luciferase and FMN:NAD(P)H Oxidoreductase

Cited by 14 publications

References 28 publications

Functionalization of DNA nanostructures with proteins

Functionalization of DNA nanostructures with proteins

Synthesis and Evaluation of the Luciferase‐Oligodeoxynucleotide for the Sequence‐Selective Detection of Nucleic Acids

Halbsynthetische DNA‐Protein‐Konjugate für Biosensorik und Nanofabrikation

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