Specific identification of Pasteurella multocida serogroup-A isolates by PCR assay

Gautam, Rajeev; Kumar, Ashok; Singh, Vijendra Pal; Dutta, T. K.; Shivachandra, Sathish Bhadravati

doi:10.1016/j.rvsc.2003.10.005

Cited by 22 publications

(20 citation statements)

References 10 publications

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“…A sample from the lung was cultured on Columbia agar (Lab M Ltd., Bury, UK) plates supplemented with 5% sheep blood under aerobic conditions at 37 °C for 24 h. The identity of the P. multocida isolate was confirmed by species-specific polymerase chain reaction (PCR) assay (Townsend et al, 1998). Combinations of oligonucleotide primers were used to amplify fragments from the kmt1 (species identification), toxA (P. multocida toxin), and hyaC-hyaD (capsular serogroup A) genes in a single, multiplex reaction (Gautam et al, 2004;Register and DeJong, 2006). The somatic serotype was established using the gel diffusion precipitin test (Heddleston et al, 1972).…”

Section: Isolation and Characterisation Of P Multocidamentioning

confidence: 99%

Characterisation of a multiresistant Pasteurella multocida strain isolated from cattle

Újvári

Makrai

Magyar

2018

Acta Veterinaria Hungarica

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The emergence of simultaneous resistance to multiple classes of antibiotics presents an increasing threat. Plasmid-borne multiresistance and integrative conjugative elements have been reported in Pasteurella multocida. We report an alternative strategy for the development of multiresistance observed in a P. multocida strain (Pm238) isolated from calf pneumonia. We identified genes integrated into the chromosomal DNA without known integrative and conjugative elements. These genes conferred resistance to streptomycin (strA), tetracycline (tetB), chloramphenicol (catAIII), and sulphonamides (sulII). We also detected mutation in the quinolone-resistance-determining regions of parC. No plasmids could be isolated from strain Pm238. These results suggest that P. multocida can accumulate multiple resistance determinants on the chromosome as single genes.

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Section: Isolation and Characterisation Of P Multocidamentioning

confidence: 99%

Characterisation of a multiresistant Pasteurella multocida strain isolated from cattle

Újvári

Makrai

Magyar

2018

Acta Veterinaria Hungarica

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“…In Europe, this capsular type is the second most prevalent (14%) of those associated with fowl cholera and related diseases (5). Type F isolates have occasionally been reported in ruminants (10,21), but information on their origin or the pathology involved is still missing. Recent work in the United Kingdom by Davies and colleagues demonstrated that only 1 of 153 bovine P. multocida strains (6) and 2 of 158 porcine P. multocida strains (7) could be assigned to capsular type F. While these two porcine isolates were associated with pneumonia, the single bovine strain was isolated from a calf with severe head and periocular edema that resembled conjunctivitis in poultry.…”

Section: Case Reportsmentioning

confidence: 99%

Fatal Peritonitis Caused by Pasteurella multocida Capsular Type F in Calves

Catry¹,

Chiers

Schwarz

et al. 2005

J Clin Microbiol

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A fatal case of atypical septicemia of pasteurellosis in veal calves is described. The causative organism was identified as a multiresistant Pasteurella multocida capsular type F isolate. The outbreak was characterized by fibrinous peritonitis and mortality, which are hitherto unreported features of P. multocida capsular type F infections. CASE REPORTSMale Holstein-Friesian calves (age, 2 weeks) were housed in individual wooden straw-bedded boxes. They were fed a milk replacer diet twice a day. The diet was supplemented with 1.5 g of oxytetracycline (Oxytem; Ecuphar) (80%) and 0.5 g of colistin (polymyxin E; Promycine Pulvis; VMD) (4,800 IU/mg) for the first 5 days. At the time of investigation (16 December 2003), 180 calves 5 to 6 weeks old were present in the herd. The calves had not received any vaccination since the day of arrival at the farm. Three calves had died on the night of 15 December 2003, one of which was subjected to necropsy on the farm and showed extended peritonitis. The remaining two calves were submitted within 8 h to the Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, for necropsy. About 15 calves in the boxes next to those of the dead calves showed nasal discharge and mild diarrhea. Every calf (weight, ca. 50 kg) of the entire herd was then orally treated (methaphylaxis) with 0.8 g of amoxicillin (Dokamox; Emdoka) (80%) twice a day for five consecutive days. The symptoms disappeared within 2 days without relapses or deaths. Routine laboratory investigation consisted of a direct identification test for antigens of rotavirus, coronavirus, Escherichia coli F5, and Cryptosporidium parvum (Digestive enzyme-linked immunosorbent assay kit; Bio-X Diagnostics) in the feces of one calf and detection of bovine viral diarrhea virus antigens by means of real-time PCR (Adiavet-BVD Realtime; Adiagène) in pooled blood samples from eight calves of the same herd. All these laboratory tests were negative.During necropsy of the two calves, samples from the cerebrum, cerebellum, brain stem, lung, mesenteric lymph nodes, synovial fluid of several joints, and omentum major were taken and processed by standard techniques for histological examination. Gross lesions were similar in both calves and consisted of an exudative fibrinous peritonitis (Fig. 1). The synovial fluid of the metacarpal, metatarsal, and elbow joint were hyperemic. The mesenteric lymph nodes were enlarged and mildly hemorrhagic. At histology, the propria of the omentum was edematous and infiltrated by moderate numbers of neutrophils. The mesothelium was covered with fibrin. The synovial fluid samples were hyperemic and edematous. A mild interstitial pneumonia was present in one calf. Lesions were not found in any of the other samples.Samples of lung tissue, peritoneal fluid, and the elbow joint were bacteriologically examined by routine standard techniques for aerobic and anaerobic bacteria as well as Mycoplasma spp. (16). In both calves, mucoid nonhemolytic gramnegative bacteria were ...

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“…Capsular/somatic serotyping either by indirect haemagglutination assay or by gel diffusion precipitation test and multiplex capsular PCR assays was used previously for identification of all of these strains Shivachandra et al, 2006a,c). The template DNAs, both bacterial culture lysate and genomic DNA, were prepared according to methods described by earlier workers (Wilson, 1987;Gautam et al, 2004), following initial growth of all bacterial cultures of P. multocida in brain-heart infusion broth (BHI). The template DNAs were subjected to rapid single-primer-PCR assay for differentiation of strains.…”

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confidence: 99%