Specific Identification of Bacillus Anthracis by Means of a Variant Bacteriophage

Brown, Eric; Cherry, William B.

doi:10.1093/infdis/96.1.34

Cited by 102 publications

(74 citation statements)

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“…The Pasteur II strain of B. anthracis was obtained from Dr. S. Makino, Obihiro University of Agriculture and Veterinary Medicine, Japan (4). The Davis strain of B. anthracis and γ-phage were obtained from the National Institute of Animal Health, Japan (3,26). All other Bacillus strains were purchased from the National Institute of Technology and Evaluation Biological Resource Center, Japan.…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, its specificity was comparable to the γ-phage test. Although the total assay time and detection limit of this method are about 3 hr and 1ϫ10 3 CFU, respectively, these could be reduced and modifications may increase the sensitivity of this method; e.g., direct enzyme conjugation to PlyGB or direct labeling of PlyGB with lanthanide chelate or stable nanocrystals, such as Quantum dot, would be useful modification methods. Further modification of the present detection system is currently in progress in our laboratory.…”

Section: B Anthracismentioning

confidence: 99%

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Sensitive Detection of Bacillus anthracis Using a Binding Protein Originating from γ‐Phage

Fujinami

Hirai

Sakai

et al. 2007

Microbiology and Immunology

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Detection of biological weapons is a primary concern in force protection, treaty verification, and safeguarding civilian populations against domestic terrorism. One great concern is the detection of Bacillus anthracis, the causative agent of anthrax. Therefore, there is a pressing need to develop novel methods for rapid, simple, and precise detection of B. anthracis. Here, we report that the C‐terminal region of γ‐phage lysin protein (PlyG) binds specifically to the cell wall of B. anthracis and the recombinant protein corresponding to this region (positions, 156–233), PlyGB, is available as a bioprobe for detection of B. anthracis. Our detection method, based on a membrane direct blot assay using recombinant PlyGB, was more rapid and sensitive than the γ‐phage test and was simpler and more inexpensive than genetic methods such as PCR, or immunological methods using specific antibodies. Furthermore, its specificity was comparable to the γ‐phage test. PlyGB is applicable in conventional methods instead of antibodies and could be a potent tool for detection of B. anthracis.

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Section: Methodsmentioning

confidence: 99%

Section: B Anthracismentioning

confidence: 99%

Sensitive Detection of Bacillus anthracis Using a Binding Protein Originating from γ‐Phage

Fujinami

Hirai

Sakai

et al. 2007

Microbiology and Immunology

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“…Lawns of the strains were prepared on plates of Nutrient Agar (Oxoid) by spread inoculating with 0.1 ml of an overnight broth culture. One loopful of y-phage (Brown and Cherry, 1955) suspension containing lo7 plaque-forming units/ml was placed in the centre of the plate. Inocula were allowed to dry and plates were incubated at 37°C for 18 h and examined for areas devoid of growth.…”

Section: Identification Of B Anthracis By Api Tests 77mentioning

confidence: 99%

“…All of the strains of B. anthracis were lysed by the y-phage of Brown and Cherry (1955); five strains (B0464, B0470, B0474, B0476 and B0478) showed some growth across the plaques, and one strain (B1125) produced a plaque that was only just visible.…”

Section: Miscellaneous Testsmentioning

confidence: 99%

“…Because of the considerable similarity between B. anthracis and B. cereus, many techniques for the selective isolation (Pearce and Powell,195 1; Morris, 1955;Green and Jamieson, 1958;Knisely, 1965 and1966) and identification (Brown and Cherry, 1955;Chadwick, 1959 and1963;Bonventre and Eckert, 1963) of B. anthracis have been developed, but none is absolutely reliable. B. cereus, although often regarded as a laboratory contaminant, is now recognised as a pathogen and isolated as such with increasing frequency (Turnbull et al, 1979; Gilbert et al, 1981).…”

Section: Introductionmentioning

confidence: 99%

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Identification of Bacillus Anthracis by Api Tests

Logan¹,

Carman

Melling

et al. 1985

Journal of Medical Microbiology

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SUMMARY. API and morphological tests were examined for their ability to distinguish between 37 Bacillus anthracis strains (virulent and avirulent) and 194 strains of closely related Bacillus species (B. cereus, B. mycoides and B. thuringiensis). In addition, 34 strains of B. anthracis and four of B. cereus were tested by several other methods that included capsule formation, ability to grow on a selective medium, and sensitivity to phage. It was found that virulent strains of B. anthracis were easily separated from the closely related Bacillus species by most of the test methods; but separation of slightly virulent and avirulent strains of B. anthracis from the closely related species could be done only by API and phage-sensi tivi ty tests.

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A Review of Antibody Capture and Bacteriophage Amplification in Connection with the Direct Analysis of Whole‐Cell Bacteria by MALDI‐TOF MS

Voorhees

Rees

2005

Identification of Microorganisms by Mass Spectrometry

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Specific Identification of Bacillus Anthracis by Means of a Variant Bacteriophage

Cited by 102 publications

References 0 publications

Sensitive Detection of Bacillus anthracis Using a Binding Protein Originating from γ‐Phage

Sensitive Detection of Bacillus anthracis Using a Binding Protein Originating from γ‐Phage

Identification of Bacillus Anthracis by Api Tests

A Review of Antibody Capture and Bacteriophage Amplification in Connection with the Direct Analysis of Whole‐Cell Bacteria by MALDI‐TOF MS

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