Specific identification and molecular typing analysis of Lactobacillus johnsonii by using PCR-based methods and pulsed-field gel electrophoresis

Ventura, Marco

doi:10.1016/s0378-1097(02)01070-4

Cited by 45 publications

(67 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Strains sharing the same pattern came from the same sample, leading us to think that they represented the same strain isolated several times. Ferrero et al (1996), Zapparoli et al (1998) and Ventura & Zink (2002) reported discriminatory, clear and reproducible RFLP-PFGE patterns in various Lactobacillus species, which allowed its use for typing studies. Our results agree with their observations.…”

Section: Numerical Analysis Of Rflp-pfge Patternsmentioning

confidence: 97%

Polyphasic study of wine Lactobacillus strains: taxonomic implications

Rodas

Ferrer

Pardo

2005

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

One hundred and seventy-eight lactobacilli isolated from wine were characterized by a polyphasic approach. Strains were phenotypically identified at genus and species level by classical tests including the analysis of cell morphology, homo/heterofermentative character, sugar fermentation patterns, growth at different temperatures and the optical nature of the isomer of lactic acid produced from glucose. Molecular techniques such as random amplification of polymorphic DNA (RAPD), amplified 16S rDNA restriction analysis (16S-ARDRA), PFGE-RFLP and ribotyping were used to characterize strains, and their potential for identification and/or typing was evaluated. The information obtained with these techniques was processed with the BioNumerics software in order to analyse relationships existing between isolated strains and various reference species of the genus. Then, taxonomic dendrograms were obtained, and this information allowed the proposal of molecular procedures suitable for the identification and typing of these wine micro-organisms. The techniques useful for both identification and typing were RAPD and ribotyping, while 16S-ARDRA was only useful for identification and PFGE-RFLP only for typing purposes. The wine strains were identified as Lactobacillus brevis (19 strains), Lactobacillus collinoides (2 strains), Lactobacillus hilgardii (71 strains), Lactobacillus paracasei (13 strains), Lactobacillus pentosus (2 strains), Lactobacillus plantarum (34 strains) and Lactobacillus mali (10 strains).

show abstract

Section: Numerical Analysis Of Rflp-pfge Patternsmentioning

confidence: 97%

Polyphasic study of wine Lactobacillus strains: taxonomic implications

Rodas

Ferrer

Pardo

2005

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

show abstract

“…longum DJO10A) followed by a second PCR amplification with primers specific for 16S rRNA genes found in all members of the genus Bifidobacterium: LM3 (5 0 -CG GGTGCTIcCCCACTTTCATG-3 0 ) and LM26 (5 0 -GAT TCTGGCTCAGGATGAACG-3 0 ) (the targeted amplicon corresponds to bases 12 to 1400 of the 16S rRNA gene of B. longum subsp. longum DJO10A) according to the PCR protocols described earlier (Kaufmann et al, 1997;Ventura & Zink, 2002).…”

Section: Construction Of 16s Rrna Gene Librariesmentioning

confidence: 99%

Microbiomic analysis of the bifidobacterial population in the human distal gut

et al. 2009

View full text Add to dashboard Cite

One of the most complex microbial ecosystems is represented by the microbiota of the human gastrointestinal tract (GIT). Although this microbial consortium has been recognized to have a crucial effect on human health, its precise composition is still not fully established. Among the GIT bacteria, bifidobacteria represent an important commensal group whose presence is often associated with health-promoting effects. In this work, we assessed the complexity of the human intestinal bifidobacterial population by analysing the diversity of several 16S rRNA gene-based libraries. These analyses showed the presence of novel bifidobacterial phylotypes, which had not been found earlier and may thus represent novel taxa within the genus Bifidobacterium.

show abstract

“…ERIC-PCR was performed using the forward primer 5'-atg taa gct cct ggg gat tca c-3' and reverse primer 5'-aag taa gtg act ggg gtg agc g-3' (Ventura & Zink 2002). Each 50 µl reaction mixture contained 5 µl 10× reaction buffer (Roche), 200 µM of each deoxynucleoside triphosphates (Invitrogen), 25 pmol of each primer (Invitrogen), 2 U Taq polymerase (Roche) and 20 ng of the respective DNA template.…”

Section: Fishmentioning

confidence: 99%

Vaccine-associated systemic Rhodococcus erythropolis infection in farmed Atlantic salmon Salmo salar

Olsen

Birkbeck²,

Nilsen³

et al. 2006

Dis. Aquat. Org.

View full text Add to dashboard Cite

In 7 instances between 2000 and 2003, clinical investigation of populations of fresh-and seawater-reared, vaccinated, Atlantic salmon Salmo salar suffering total losses of between 0.1 and 35% revealed infection with a Gram-positive rod-shaped bacterium. The isolations were geographically widespread, occurring in both Norway and Scotland. In all cases, a Gram-positive bacterium, subsequently identified as Rhodococcus erythropolis, was isolated in pure culture. Infections, although systemic, were focused within the peritoneal cavity. While initial attempts to reproduce the disease by intraperitoneal injection of unvaccinated Atlantic salmon failed, Koch's postulates were subsequently fulfilled in fish vaccinated with a commercially available oil-adjuvanted vaccine. MATERIALS AND METHODS Fish.Moribund fish from all 7 disease outbreaks (see Table 1) were sampled for pathological and bacteriological examination.Histopathology. Gills, heart, liver, kidney, spleen, pancreatic tissue, skeletal musculature and occasionally brain were sampled for histopathology. The samples were fixed in 4% neutral buffered formalin, embedded in paraffin wax and routinely processed. The sections were stained by haematoxylin and eosin (H&E). A selected number of slides were also stained using Gram and May Grünwald Giemsa stains.Bacteriology. For bacteriological examination, samples from kidney and in some cases ascitic fluid, were inoculated onto blood agar (BA, 4% bovine or equine blood ) and blood agar supplemented with 1.5% NaCl (BAS) followed by aerobic incubation at 22 and 15°C, respectively. Plates were observed for 7 d. Bacterial isolates were subsequently stored at -80°C.Biochemical characterisation. Basic biochemical characterisation was performed using standard methods. Each strain was also tested using BIOLOG (Hayward) and API 20 NE (Biomerieux) kits according to the manufacturers' instructions. The ability of each strain to assimilate various carbon sources was examined by inoculating API 50 CH kits (Biomerieux) with bacteria suspended in a medium comprising 1.5 g agar, 2.0 g ammonium sulphate, 0.25 g Casamino acids (Difco) and 1 ml trace element solution.DNA sequencing. The 16S rRNA gene of Strains 00/50/6670 and 4115 were amplified using PCR and primers FD1 and RP2 (Weisburg et al. 1991). DNA sequencing was performed using the BigDye™ terminator cycle sequencing ready reaction kit (PE Applied Biosystems) and an automatic ABI prism 377 sequencer (Perkin Elmer). Sequence analysis was performed using the sequencer program (Gene Codes) and BLAST search analysis (Altschul et al. 1997).Phylogenetic analysis. Sequences were aligned using CLUSTAL W (Thompson et al. 1994). A neighbour-joining tree based on Kimura 2-parameter distances was calculated using PAUP* 4.0 (Swofford 2000). The tree was bootstrapped 1000 times to assess node reliability.Enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). ERIC-PCR was performed using the forward primer 5'-atg taa gct cct ggg gat tca c-3' and reverse primer 5'-aag taa gtg...

show abstract

Specific identification and molecular typing analysis of Lactobacillus johnsonii by using PCR-based methods and pulsed-field gel electrophoresis

Cited by 45 publications

References 22 publications

Polyphasic study of wine Lactobacillus strains: taxonomic implications

Polyphasic study of wine Lactobacillus strains: taxonomic implications

Microbiomic analysis of the bifidobacterial population in the human distal gut

Vaccine-associated systemic Rhodococcus erythropolis infection in farmed Atlantic salmon Salmo salar

Contact Info

Product

Resources

About