Specific growth rate of Chlamydomonas reinhardtii and Chlorella sorokiniana under medium duration light/dark cycles: 13–87 s

Janssen, Marcel; Kuijpers, Tjibbe Chris; Veldhoen, Bram; Ternbach, Michel Brik; Tramper, J.; Mur, Luuc R.; Wijffels, René H.

doi:10.1016/s0079-6352(99)80124-6

Cited by 16 publications

(13 citation statements)

References 24 publications

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“…Previous work has shown that flashes having sub-second light-dark cycles (ƒ > 1 s −1 ) led to higher photosynthetic efficiency than constant light of the same LI, as long as LI was saturating, that is, in the section of the growth-irradiance curve where additional light did not accelerate the growth rate (Janssen et al, 2001;Vejrazka et al, 2011). However, similar studies found that longer flashes (f < 0.2 s −1 ) gave lower photosynthetic efficiency (Janssen et al, 1999(Janssen et al, , 2000.…”

Section: Introduction Results and Discussionmentioning

confidence: 91%

Growth kinetics and mathematical modeling of Synechocystis sp. PCC 6803 under flashing light

Straka

Rittmann

2018

Biotech & Bioengineering

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In photobioreactors and natural systems, microalgae are subjected to rapidly changing light intensities (LI) due to light attenuation and mixing. A controlled way to study the effect of rapidly changing LI is to subject cultures to flashing light. In this study, series of flashing-light experiments were conducted using Synechocystis sp.PCC6803 with constant overall average LI of approximately 84 μmol·m −2 ·s −1 and relative times in the light and dark varied. The results were also compared with simulated results using a mathematical model including an absorbed pool of light energy, photoacclimation, and photoinhibition. With equal time in light and dark, the specific growth rate (μ) systematically decreased with increasing light duration, and µ decreased further when the ratio of light to dark was decreased. The model captured both trends with the mechanistic explanation that when the light duration was very short the changes in the pool of absorbed LI were smoothed out across the light and dark periods, whereas longer durations caused the biomass to experience discrete light and dark conditions that lead to reduced light absorption, more energy loss to nonphotochemical quenching, and more photodamage. These growth effects were accentuated as the ratio of light to dark decreased. K E Y W O R D S flashing light, light-dependent kinetics, mathematical modeling, microalgae, Synechocystis sp.PCC6803

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Section: Introduction Results and Discussionmentioning

confidence: 91%

Growth kinetics and mathematical modeling of Synechocystis sp. PCC 6803 under flashing light

Straka

Rittmann

2018

Biotech & Bioengineering

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“…In cultivation of microalgae using photobioreactors, excessive light energy supplied would not only increase total electricity consumption but also inhibit the growth of microalgae 22. To overcome this problem, the photobioreactor was illuminated at different light/dark cycle to avoid excessive illumination and to save the energy (electricity) cost.…”

Section: Resultsmentioning

confidence: 99%

Strategies to enhance cell growth and achieve high‐level oil production of a Chlorella vulgaris isolate

Chen

Yeh

Su³

et al. 2010

Biotechnology Progress

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The autotrophic growth of an oil-rich indigenous microalgal isolate, identified as Chlorella vulgaris C--C, was promoted by using engineering strategies to obtain the microalgal oil for biodiesel synthesis. Illumination with a light/dark cycle of 14/10 (i.e., 14 h light-on and 10 h light-off) resulted in a high overall oil production rate (v(oil)) of 9.78 mg/L/day and a high electricity conversion efficiency (E(c)) of 23.7 mg cell/kw h. When using a NaHCO(3) concentration of 1,500 mg/L as carbon source, the v(oil) and E(c) were maximal at 100 mg/L/day and 128 mg/kw h, respectively. A Monod type model was used to describe the microalgal growth kinetics with an estimated maximum specific growth rate (mu(max)) of 0.605 day(-1) and a half saturation coefficient (K(s)) of 124.9 mg/L. An optimal nitrogen source (KNO(3)) concentration of 625 mg/L could further enhance the microalgal biomass and oil production, leading to a nearly 6.19 fold increase in v(oil) value.

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“…The latter case typically arises in an illuminated PBR presenting dark volumes deep in the culture. Because of hydrodynamics and cell displacement, cells encounter fluctuating light regimes (light/dark cycles) 21, 31, 32. This can result in complex interactions between photosynthesis and respiration, depending on the species and cell history with respect to light.…”

Section: Theoretical Considerationsmentioning

confidence: 99%

“…Unlike cell shape and size, pigment content was found to be significantly affected by the incident PFD (Figure 4). This well‐known phenomenon, called photoacclimation, can be induced by a modification of spectral distribution or light intensity, leading to a decrease in pigment contents with increasing PFD 12, 32, 34, 35. Values are given in Figure 4 for the wide range of PFDs covered in experiments (100–1,000 μmol m −2 s −1 ).…”

Section: Theoretical Considerationsmentioning

confidence: 99%

Kinetic modeling of the photosynthetic growth of Chlamydomonas reinhardtii in a photobioreactor

Takache

Pruvost

Cornet

2012

Biotechnology Progress

105

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The aim of this study was to establish and validate a model for the photosynthetic growth of Chlamydomonas reinhardtii in photobioreactors (PBRs). The proposed model is based on an energetic analysis of the excitation energy transfer in the photosynthesis apparatus (the Z-scheme for photosynthesis). This approach has already been validated in cyanobacteria (Arthorspira platensis) and is extended here to predict the volumetric biomass productivity for the microalga C. reinhardtii in autotrophic conditions, taking into consideration the two metabolic processes taking place in this eukaryotic microorganism, namely photosynthesis and respiration. The kinetic growth model obtained was then coupled to a radiative transfer model (the two-flux model) to determine the local kinetics, and thereby the volumetric biomass productivity, in a torus PBR. The model was found to predict PBR performances accurately for a broad set of operating conditions, including both light-limited and kinetic growth regimes, with a variance of less than 10% between experimental results and simulations.

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Specific growth rate of Chlamydomonas reinhardtii and Chlorella sorokiniana under medium duration light/dark cycles: 13–87 s

Cited by 16 publications

References 24 publications

Growth kinetics and mathematical modeling of Synechocystis sp. PCC 6803 under flashing light

Growth kinetics and mathematical modeling of Synechocystis sp. PCC 6803 under flashing light

Strategies to enhance cell growth and achieve high‐level oil production of a Chlorella vulgaris isolate

Kinetic modeling of the photosynthetic growth of Chlamydomonas reinhardtii in a photobioreactor

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