Specific Frequencies of Spontaneous Ca2+ Transients Upregulate GAD 67 Transcripts in Embryonic Spinal Neurons

Watt, Stephen D.; Gu, Xianfeng; Smith, Raymond D.; Spitzer, Nicholas C.

doi:10.1006/mcne.2000.0871

Cited by 64 publications

(44 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, we confidently establish that the expression of the GABAergic markers in adult GC cultures depends on prolonged increases of excitability, with the concomitant calcium entry, and on protein synthesis, as we have previously shown in GCs in situ (Gutiérrez, 2002). The role of calcium on phenotypic differentiation, and especially on the expression of the GABAergic phenotype, has been documented in developing cells (Watt et al, 2000;Ciccolini et al, 2003;Borodinsky et al, 2004).…”

Section: Discussionsupporting

confidence: 78%

Programmed and Induced Phenotype of the Hippocampal Granule Cells

Gómez-Lira¹,

Lamas²,

Romo-Parra³

et al. 2005

J. Neurosci.

View full text Add to dashboard Cite

Certain neurons choose the neurotransmitter they use in an activity-dependent manner, and trophic factors are involved in this phenotypic differentiation during development. Developing hippocampal granule cells (GCs) constitutively express the markers of the glutamatergic and GABAergic phenotypes, but when development is completed, the GABAergic phenotype shuts off. With electrophysiological, single-cell reverse transcription-PCR and immunohistological techniques, we show here that short-term (24 h) cultures of fully differentiated adult glutamatergic GCs, which express glutamate, VGlut-1 (vesicular glutamate transporter) mRNA, calbindin, and dynorphin mRNA, can be induced to reexpress the GABAergic markers GABA, GAD 67 (glutamate decarboxylase 67 kDa isoform), and VGAT (vesicular GABA transporter) mRNA, by sustained synaptic or direct activation of glutamate receptors and by activation of TrkB (tyrosine receptor kinase B) receptors, with brain-derived neurotrophic factor (BDNF) (30 min). The expression of the GABAergic markers was prevented by the blockade of glutamate receptors and sodium or calcium channels, and by inhibitors of protein kinases and protein synthesis. In hippocampal slices of epileptic rats and in BDNF-treated slices from naive rats, we confirmed the appearance of monosynaptic GABA A receptor-mediated responses to GC stimulation, in the presence of glutamate receptors blockers. Accordingly, GC cultures prepared from these slices showed the coexpression of the glutamatergic and GABAergic markers. Our results demonstrate that the neurotransmitter choice of the GCs, which are unique in terms of their continuing birth and death throughout life, depends on programmed and environmental factors, and this process is neither limited by a critical developmental period nor restricted by their insertion in their natural network.

show abstract

Section: Discussionsupporting

confidence: 78%

Programmed and Induced Phenotype of the Hippocampal Granule Cells

Gómez-Lira¹,

Lamas²,

Romo-Parra³

et al. 2005

J. Neurosci.

View full text Add to dashboard Cite

show abstract

“…There is precedent for such strong, temporally spaced stimuli to activate specific intracellular signaling pathways (Wu et al, 2001). Furthermore both the pattern and frequency of stimulation and ensuing Ca 2ϩ transients can regulate neuronal gene expression (Dolmetsch et al, 1997;Itoh et al, 1997;Watt et al, 2000). Thus, the frequency of the bursting episodes may be the critical variable to which motoneurons are responding.…”

Section: A-p/motoneuron Pool-specific Fasciculation and Pathfinding Ementioning

confidence: 99%

Increasing the Frequency of Spontaneous Rhythmic Activity Disrupts Pool-Specific Axon Fasciculation and Pathfinding of Embryonic Spinal Motoneurons

Hanson¹,

Landmesser²

2006

J. Neurosci.

View full text Add to dashboard Cite

Rhythmic spontaneous bursting activity, which occurs in many developing neural circuits, has been considered to be important for the refinement of neural projections but not for early pathfinding decisions. However, the precise frequency of bursting activity differentially affects the two major pathfinding decisions made by chick lumbosacral motoneurons. Moderate slowing of burst frequency was shown previously to cause motoneurons to make dorsoventral (D-V) pathfinding errors and to alter the expression of molecules involved in that decision. Moderate speeding up of activity is shown here not to affect these molecules or D-V pathfinding but to strongly perturb the anteroposterior (A-P) pathfinding process by which motoneurons fasciculate into pool-specific fascicles at the limb base and then selectively grow to muscle targets. Resumption of normal frequency allowed axons to correct the A-P pathfinding errors by altering their trajectories distally, indicating the dynamic nature of this process and its continued sensitivity to patterned activity.

show abstract

“…Currently, many studies demonstrate that multiple cellular events are tuned to respond optimally to specific frequencies and patterns of Ca 2? spiking ( (Berridge et al 2000;Frey et al 2000;Watt et al 2000;Reither et al 2006;Zhu et al 2008), but the mechanisms underlying their formation and dynamic characteristics are diverse and remained to be explained (Frey et al 2000;Carafoli 2002;Berridge et al 2003). In this regard, investigation in cell lines in vitro, particularly in HEK293 cells, has appeared to be one of the important approaches for obtaining evidence of and information on this type of Ca …”

Section: Intracellularmentioning

confidence: 99%

“…In addition, more recent studies indicate that some specific intracellular second messengers and a variety of physiological processes are finely modulated by the information encoded in the frequency, amplitude and duration of [Ca 2? ] i transients (Thomas et al 1996;Watt et al 2000;Reither et al 2006;Willoughby and Cooper 2006;Zhu et al 2008). Therefore, it is important to investigate the dynamic characteristics and underling mechanism(s) hidden in this particular Ca 2?…”

Section: Introductionmentioning

confidence: 99%