Specific Fluorescent Probes for Imaging DNA in Cell-Free Solution and in Mitochondria in Living Cells

Abstract

New styryl dyes consisting of N-methylpyridine or N-methylquinoline scaffolds were synthesized, and their binding affinities for DNA in cell-free solution were studied. The replacement of heterocyclic residue from the pyridine to quinoline group as well as variation in the phenyl part strongly influenced their binding modes, binding affinities, and spectroscopic responses. Biological experiments showed the low toxicity of the obtained dyes and their applicability as selective dyes for mitochondria in living cells.

Design and Synthesis of Novel Squaraine-Based Fluorescent Probe for Far-Red Detection of Chymotrypsin Enzyme

“…In Figure 3c,d F In our study, we explored the fluorescence properties of SQ-122 PC under different conditions using fluorescence microscopy [39,40], focusing on the impact of chymotrypsin. We compared fluorescence images obtained for SQ-122 PC under two conditions (Figure 4a,b).…”

“…This enhancement in the fluorescence signal suggests the occurrence of FRET off, indicating a change in the energy transfer dynamics within the dye-peptide system. In our study, we explored the fluorescence properties of SQ-122 PC under different conditions using fluorescence microscopy [39,40], focusing on the impact of chymotrypsin. We compared fluorescence images obtained for SQ-122 PC under two conditions (Figure 4a,b).…”