Specific expression of annexin A8 in adult murine stratified epithelia

Abstract

Annexin A8 is a member of the annexin family of calcium-regulated membrane-binding proteins. In this report, we investigated the expression of annexin A8 in adult mouse organs. Northern blot analysis of adult mouse organs showed that a single annexin A8 transcript of 1.9 kb is expressed most strongly in skin, eye and tongue. In situ hybridisations using annexin A8-specific probes revealed that in the stratified epithelia of the tongue and the early postnatal epidermis, annexin A8 transcription could be detected in basal and suprabasal layers of these stratified epithelia. Western blot analyses using a murine ANXA8-specific antiserum showed, that the 36 kD ANXA8 protein was most abundant in the skin and tongue. The abundance of ANXA8 protein in the skin increased during postnatal days 1-18 and was immunohistochemically localised in suprabasal layers of the epidermis. In the tongue epithelium as well, ANXA8 protein was found in suprabasal layers. ANXA8 immunoreactivity was also found in suprabasal layers of the stratified epithelia of the oesophagus and the forestomach, while it was detected in all layers of the cornea epithelium and in the cornea endothelium of the eye. We also investigated the expression of retinoic acid receptor alpha protein (RARA) and ANXA8 in the epidermis immunohistochemically. While RARA immunoreactivity was exclusively detected in the basal layer, ANXA8 immunoreactivity was restricted to suprabasal layers of the epidermis. Thus, ANXA8 protein is most abundant in stratified epithelia of the postnatal mouse. Its location in the suprabasal layers suggests that ANXA8 may be associated with the terminal differentiation of epithelial cells in these tissues.

Annexin A8 Is a Prognostic Marker and Potential Therapeutic Target for Pancreatic Cancer

“…16 Our study displayed that A-A8 may have a role in cellular differentiation, and we found that A-A8 was remarkably upregulated in pancreatic cancer cells, with a 5.9-fold increased expression of A-A8 from low- to high-grade tumors. Additionally, our study showed that immunohistochemical expression of A-A8 was correlated with tumor grade in a human pancreatic tumor tissue microarray and that increased levels of A-A8 protein expression were significantly associated with high-grade tumors.…”

Pemphigus Vulgaris Autoantibody Profiling by Proteomic Technique

“…annexin 9) [69]. Annexin A8 is specifically expressed in adult stratified epithelia [70], where it may participate in the organization of certain actin associated membrane domains and regulate late endosome organization and function [71]. By analogy to annexins 1, 2, 3 and 9 that act as non-professional AChRs [69], [72], the annexin A8-like molecule also may mediate acetylcholine signaling and, thus, be involved in regulation of keratinocyte shape and adhesion.…”

Gene expression analysis of an epidermolysis bullosa simplex Dowling‐Meara cell line by subtractive hybridization: recapitulation of cellular differentiation, migration and wound healing

“…TXNIP interacts with sciellin, a precursor of the cornified envelope, and is assumed to have a role in regulating the transition of transient amplifying cells to suprabasal differentiating cells in epithelial tissues (80). As ANXA8 also has been found to be most abundant in the stratified epithelia of the epidermis, the forestomach, the oesophagus, the tongue and the cornea, it has been suggested that it plays a role in epithelial differentiation (81). ANXA8 was proposed to have an anticoagulatory activity in lung endothelial cells (82,83) and to participate in the interaction between the plasma membrane and the cytoskeleton owing to its high specificity binding to phosphatidylinositol (4,5)-bisphosphate and interaction with F-actin in HeLa cells (84).…”