Specific Enzyme Immobilization Approaches and Their Application with Nanomaterials

Liu, Wen-Shan; Wang, Liang; Jiang, Rongrong

doi:10.1007/s11244-012-9893-0

Cited by 62 publications

(38 citation statements)

References 99 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although most of the covalent immobilization methods are nonspecific, new strategies have overcome this problem [53] using direct enzyme modification, such as bioeorthogonal reactions for site-specific protein labeling [54,55]. "Click chemistry", for example, has been used extensively used in modern chemistry, especially for immobilizing azido-or alkyne-containing proteins onto alkyne-or azido-coated surfaces, respectively [56].…”

Section: Covalent Linkingmentioning

confidence: 99%

Protein functionalized carbon nanomaterials for biomedical applications

Oliveira

Bisker

Bakh

et al. 2015

Carbon

185

109

View full text Add to dashboard Cite

a b s t r a c tSince the discovery of low-dimensional carbon allotropes, there is increasing interest in using carbon nanomaterials for biomedical applications. Carbon nanomaterials have been utilized in the biomedical field for bioimaging, chemical sensing, targeting, delivery, therapeutics, catalysis, and energy harvesting. Each application requires tailored surface functionalization in order to take advantage of a desired property of the nanoparticles. Herein, we review the surface immobilization of bio-molecules, including proteins, peptides, and enzymes, and present the recent advances in synthesis and applications of these conjugates. The carbon scaffold and the biological moiety form a complex interface which presents a challenge for achieving efficient and robust binding while preserving biological activity. Moreover, some applications require the utilization of the protein-nanocarbon system in a complex environment that may hinder its performance or activity. We analyze different strategies to overcome these challenges when using carbon nanomaterials as protein carriers, explore various immobilization techniques along with characterization methods, and present recent demonstrations of employing these systems for biomedical applications. Finally, we consider the challenges and future directions of this field.

show abstract

Section: Covalent Linkingmentioning

confidence: 99%

Protein functionalized carbon nanomaterials for biomedical applications

Oliveira

Bisker

Bakh

et al. 2015

Carbon

185

109

View full text Add to dashboard Cite

show abstract

“…In recent years, nanoparticles have attracted more attention due to their excellent properties and interesting applications [Mateo et al, 2007;Liu et al, 2012].…”

Section: Introductionmentioning

confidence: 99%

Stability Improvement of Immobilized Alkaline Phosphatase Using Chitosan Nanoparticles

Jafary

Panjehpour

Varshosaz

et al. 2016

Braz. J. Chem. Eng.

View full text Add to dashboard Cite

-Enzyme engineering via immobilization techniques is a suitable approach for improving enzyme function and stability and is superior to the other chemical or biological methods. In this study chitosan nanoparticles were synthesized using the Ionic Gelation method and were characterized by Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). Alkaline phosphatase was successfully immobilized on the chitosan nanoparticles in optimum conditions. Chitosan nanoparticles were used because of their special properties for enzyme immobilization. This study indicated that the immobilized enzyme has improved function at high temperature and during storage. Immobilization resulted in an increased range of optimum pH and temperature, and reusability of enzyme. Furthermore, the binding efficiency calculation indicated that the immobilized alkaline phosphatase conserved 71% of its native activity. Kinetic parameter studies indicated no significant difference between the immobilized and free enzymes.

show abstract

“…3,4 A subgroup of solid-phase bioreactors called immobilized microfluidic enzymatic reactors (IMERs) 5,6 comprises systems in which an enzyme is immobilized within the channels of a microfluidic device. There are several advantages associated with these systems as opposed to their homogeneous (liquid-based) counterparts.…”

mentioning

confidence: 99%

Immobilization of Lambda Exonuclease onto Polymer Micropillar Arrays for the Solid-Phase Digestion of dsDNAs

Oliver-Calixte

Uba²,

Battle

et al. 2014

Anal. Chem.

View full text Add to dashboard Cite

The process of immobilizing enzymes onto solid supports for bioreactions has some compelling advantages compared to their solution-based counterpart including the facile separation of enzyme from products, elimination of enzyme autodigestion, and increased enzyme stability and activity. We report the immobilization of λ-exonuclease onto poly(methylmethacrylate) (PMMA) micropillars populated within a microfluidic device for the on-chip digestion of double-stranded DNA. Enzyme immobilization was successfully accomplished using 3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling to carboxylic acid functionalized PMMA micropillars. Our results suggest that the efficiency for the catalysis of dsDNA digestion using λ-exonuclease, including its processivity and reaction rate, were higher when the enzyme was attached to a solid support compared to the free solution digestion. We obtained a clipping rate of 1.0 × 103 nucleotides s–1 for the digestion of λ-DNA (48.5 kbp) by λ-exonuclease. The kinetic behavior of the solid-phase reactor could be described by a fractal Michaelis–Menten model with a catalytic efficiency nearly 17% better than the homogeneous solution-phase reaction. The results from this work will have important ramifications in new single-molecule DNA sequencing strategies that employ free mononucleotide identification.

show abstract

Specific Enzyme Immobilization Approaches and Their Application with Nanomaterials

Cited by 62 publications

References 99 publications

Protein functionalized carbon nanomaterials for biomedical applications

Protein functionalized carbon nanomaterials for biomedical applications

Stability Improvement of Immobilized Alkaline Phosphatase Using Chitosan Nanoparticles

Immobilization of Lambda Exonuclease onto Polymer Micropillar Arrays for the Solid-Phase Digestion of dsDNAs

Contact Info

Product

Resources

About