Specific enrichment of hyperthermophilic electroactive Archaea from deep-sea hydrothermal vent on electrically conductive support

Pillot, Guillaume; Frouin, Eléonore; Pasero, Emilie; Godfroy, Anne; Combet-Blanc, Yannick; Davidson, Sylvain; Liebgott, Pierre-Pol

doi:10.1016/j.biortech.2018.03.053

Cited by 19 publications

(11 citation statements)

References 53 publications

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“…Moreover, Archaeoglobus fulgidus has been recently shown to grow on iron by directly snatching electrons under carbon starvation during the corrosion process 20 . Furthermore, Ferroglobus and Geoglobus species were shown to be exoelectrogens in pure culture in a microbial electrosynthesis cell 12 and have been enriched within a microbial electrolysis cell 11 , 13 . Given these elements, the identified Archaeoglobales species could be, under our electrolithoautotrophic conditions, the first colonizers of the electrode during the first days of growth.…”

Section: Discussionmentioning

confidence: 99%

“…The two main hypotheses are the use of similar direct electron transfer pathway as on the anode 9 , or the use of free cell-derived enzymes, which can interact with electrode surfaces to catalyze the electron transfers 10 . Recent studies have shown the exoelectrogenic ability of some hyperthermophilic microorganisms isolated from deep-sea hydrothermal vents belonging to Archaeoglobales and Thermococcales 11 – 13 , but no studies have been done on environmental samples potentially harboring electrotrophic communities growing naturally with an electric current as their sole energy source.…”

Section: Introductionmentioning

confidence: 99%

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Identification of enriched hyperthermophilic microbial communities from a deep-sea hydrothermal vent chimney under electrolithoautotrophic culture conditions

Pillot

Ali

Davidson

et al. 2021

Sci Rep

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Deep-sea hydrothermal vents are extreme and complex ecosystems based on a trophic chain. We are still unsure of the identities of the first colonizers of these environments and their metabolism, but they are thought to be (hyper)thermophilic autotrophs. Here we investigate whether the electric potential observed across hydrothermal chimneys could serve as an energy source for these first colonizers. Experiments were performed in a two-chamber microbial electrochemical system inoculated with deep-sea hydrothermal chimney samples, with a cathode as sole electron donor, CO2 as sole carbon source, and nitrate, sulfate, or oxygen as electron acceptors. After a few days of culturing, all three experiments showed growth of electrotrophic biofilms consuming the electrons (directly or indirectly) and producing organic compounds including acetate, glycerol, and pyruvate. Within the biofilms, the only known autotroph species retrieved were members of Archaeoglobales. Various heterotrophic phyla also grew through trophic interactions, with Thermococcales growing in all three experiments as well as other bacterial groups specific to each electron acceptor. This electrotrophic metabolism as energy source driving initial microbial colonization of conductive hydrothermal chimneys is discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of enriched hyperthermophilic microbial communities from a deep-sea hydrothermal vent chimney under electrolithoautotrophic culture conditions

Pillot

Ali

Davidson

et al. 2021

Sci Rep

Self Cite

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“…Moreover, electrochemically active microorganisms can utilize various substrates and have been found living in a wide range of temperatures and pH. For instance, it was found that a hyper‐thermophilic electrochemically active Archaea can work even at 80 °C, [ 22 ] and some BESs also exhibited considerable performance even at low temperature (4 °C). [ 23 ]…”

Section: Introductionmentioning

confidence: 99%

Nanomaterials Facilitating Microbial Extracellular Electron Transfer at Interfaces

Wang

Sun

et al. 2020

Advanced Materials

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Electrochemically active bacteria can transport their metabolically generated electrons to anodes, or accept electrons from cathodes to synthesize high‐value chemicals and fuels, via a process known as extracellular electron transfer (EET). Harnessing of this microbial EET process has led to the development of microbial bio‐electrochemical systems (BESs), which can achieve the interconversion of electrical and chemical energy and enable electricity generation, hydrogen production, electrosynthesis, wastewater treatment, desalination, water and soil remediation, and sensing. Here, the focus is on the current understanding of the microbial EET process occurring at both the bacteria–electrode interface and the biotic interface, as well as some attempts to improve the EET by using various nanomaterials. The behavior of nanomaterials in different EET routes and their influence on the performance of BESs are described. The inherent mechanisms will guide rational design of EET‐related materials and lead to a better understanding of EET mechanisms.

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“…Very few studies have reported on the diversity of EAMs from extreme environments. These include, for instance, highly saline 17 – 19 , extreme acidic 20 and alkaline 21 , extreme low 22 and high temperature 19 , 23 , high temperature and pressure 24 , 25 , and deep subsurface 26 habitats. A combination of some of these extreme conditions also exists in some environments.…”

Section: Introductionmentioning

confidence: 99%

Microbial electroactive biofilms dominated by Geoalkalibacter spp. from a highly saline–alkaline environment

Yadav

Patil

2020

npj Biofilms Microbiomes

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Understanding of the extreme microorganisms that possess extracellular electron transfer (EET) capabilities is pivotal to advance electromicrobiology discipline and to develop niche-specific microbial electrochemistry-driven biotechnologies. Here, we report on the microbial electroactive biofilms (EABs) possessing the outward EET capabilities from a haloalkaline environment of the Lonar lake. We used the electrochemical cultivation approach to enrich haloalkaliphilic EABs under 9.5 pH and 20 g/L salinity conditions. The electrodes controlled at 0.2 V vs. Ag/AgCl yielded the best-performing biofilms in terms of maximum bioelectrocatalytic current densities of 548 ± 23 and 437 ± 17 µA/cm2 with acetate and lactate substrates, respectively. Electrochemical characterization of biofilms revealed the presence of two putative redox-active moieties with the mean formal potentials of 0.183 and 0.333 V vs. Ag/AgCl, which represent the highest values reported to date for the EABs. 16S-rRNA amplicon sequencing of EABs revealed the dominance of unknown Geoalkalibacter sp. at ~80% abundance. Further investigations on the haloalkaliphilic EABs possessing EET components with high formal potentials might offer interesting research prospects in electromicrobiology.

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Specific enrichment of hyperthermophilic electroactive Archaea from deep-sea hydrothermal vent on electrically conductive support

Cited by 19 publications

References 53 publications

Identification of enriched hyperthermophilic microbial communities from a deep-sea hydrothermal vent chimney under electrolithoautotrophic culture conditions

Identification of enriched hyperthermophilic microbial communities from a deep-sea hydrothermal vent chimney under electrolithoautotrophic culture conditions

Nanomaterials Facilitating Microbial Extracellular Electron Transfer at Interfaces

Microbial electroactive biofilms dominated by Geoalkalibacter spp. from a highly saline–alkaline environment

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