Specific Downregulation of the Bacterial-Type PEPC Gene by Artificial MicroRNA Improves Salt Tolerance in Arabidopsis

Wang, Fulin; Liu, Renhu; Guan-ting, WU; Lang, Chunxiu; Chen, Jinqing; Shi, Chao

doi:10.1007/s11105-012-0418-6

Cited by 18 publications

(8 citation statements)

References 38 publications

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“…A recent study show amiRNAs also can be used to silence the endogenous miRNAs for both miRNA families as well an individual miRNA based on where the amiRNA targeting on (Eamens et al, ). A study shows that expression of an amiRNA, targeting Cucumber mosaic virus (CMV) silencing suppressor 2b efficiently inhibited 2b gene expression and conferred transgenic tobacco plants effective resistance to CMV infection (Qu et al, ); another study shows that amiRNA‐based specific downregulation of the bacterial‐type phosphoenolpyruvate carboxylases (PEPC) gene improved plant tolerance to salinity stress in Arabidopsis (Wang et al, ). These evidences suggest that amiRNA technology can be used to create transgenic plants for improving crop tolerance to abiotic and biotic stresses.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

MicroRNA‐Based Biotechnology for Plant Improvement

Zhang

Wang

2014

Journal Cellular Physiology

202

123

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MicroRNAs (miRNAs) are an extensive class of newly discovered endogenous small RNAs, which negatively regulate gene expression at the post-transcription levels. As the application of next-generation deep sequencing and advanced bioinformatics, the miRNA-related study has been expended to non-model plant species and the number of identified miRNAs has dramatically increased in the past years. miRNAs play a critical role in almost all biological and metabolic processes, and provide a unique strategy for plant improvement. Here, we first briefly review the discovery, history, and biogenesis of miRNAs, then focus more on the application of miRNAs on plant breeding and the future directions. Increased plant biomass through controlling plant development and phase change has been one achievement for miRNA-based biotechnology; plant tolerance to abiotic and biotic stress was also significantly enhanced by regulating the expression of an individual miRNA. Both endogenous and artificial miRNAs may serve as important tools for plant improvement.

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Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

MicroRNA‐Based Biotechnology for Plant Improvement

Zhang

Wang

2014

Journal Cellular Physiology

202

123

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“…In the male gametophyte of Lilium Longiflorum, BTPC forms a complex with PTPC and monoubiquitinated PTPC to accelerate the accumulation of storage substances during pollen maturation (Igawa et al, 2010). In Arabidopsis, the downregulation of BTPC gene might increase the expression of the other three PTPC genes, indicating a transcriptional interaction between BTPC and PTPC in vascular plants (Wang et al, 2012). In this study, the transcriptional levels of three SaPEPC genes in S. aralocaspica were analyzed based on the database.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Analysis of the Phosphoenolpyruvate Carboxylase Gene Family in Suaeda aralocaspica, an Annual Halophyte With Single-Cellular C4 Anatomy

et al. 2021

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Phosphoenolpyruvate carboxylase (PEPC) plays pivotal roles in the carbon fixation of photosynthesis and a variety of metabolic and stress pathways. Suaeda aralocaspica belongs to a single-cellular C4 species and carries out a photosynthetic pathway in an unusually elongated chlorenchyma cell, which is expected to have PEPCs with different characteristics. To identify the different isoforms of PEPC genes in S. aralocaspica and comparatively analyze their expression and regulation patterns as well as the biochemical and enzymatic properties in this study, we characterized a bacterial-type PEPC (BTPC; SaPEPC-4) in addition to the two plant-type PEPCs (PTPCs; SaPEPC-1 and SaPEPC-2) using a genome-wide identification. SaPEPC-4 presented a lower expression level in all test combinations with an unknown function; two SaPTPCs showed distinct subcellular localizations and different spatiotemporal expression patterns but positively responded to abiotic stresses. Compared to SaPEPC-2, the expression of SaPEPC-1 specifically in chlorenchyma cell tissues was much more active with the progression of development and under various stresses, particularly sensitive to light, implying the involvement of SaPEPC-1 in a C4 photosynthetic pathway. In contrast, SaPEPC-2 was more like a non-photosynthetic PEPC. The expression trends of two SaPTPCs in response to light, development, and abiotic stresses were also matched with the changes in PEPC activity in vivo (native) or in vitro (recombinant), and the biochemical properties of the two recombinant SaPTPCs were similar in response to various effectors while the catalytic efficiency, substrate affinity, and enzyme activity of SaPEPC-2 were higher than that of SaPEPC-1 in vitro. All the different properties between these two SaPTPCs might be involved in transcriptional (e.g., specific cis-elements), posttranscriptional [e.g., 5′-untranslated region (5′-UTR) secondary structure], or translational (e.g., PEPC phosphorylation/dephosphorylation) regulatory events. The comparative studies on the different isoforms of the PEPC gene family in S. aralocaspica may help to decipher their exact role in C4 photosynthesis, plant growth/development, and stress resistance.

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“…This expression pattern is happening in order to limit the growth and development and the activate apoptosis mechanism. Phosphorylation is a mechanism which is phosphate can be added or remove by protein kinases and activate functional proteins [27].…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Differentially Expressed miRNAs in Leaves of Three Sugarcane (Saacharum Officinarum L.) Cultivars During Salinity Stress

Mazalmazraei

Nejadsadeghi

Mehdikhanlou

et al. 2021

Preprint

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Sugarcane is an important industrial plant which cultivated in the most arid and semiarid regions. Due to climate change and anthropogenic activities, the sugarcane field damage due to salt deposition and the cultivation of sugarcane has been posed a major threat in the region. To address this issue, the identification of salinity tolerant cultivars would be a suitable strategy to minimize yield loss in the area. MicroRNAs (miRNAs) play important roles in regulating gene expression. The monitoring of the expression of miRNAs and their targeted genes could provide deeper insight into the molecular stress mechanism and screen tolerant cultivars. Our aim was to assess the expression of nine candidate miRNAs and their corresponding targeted genes among the studied sugarcane cultivars under salinity condition, leading to identify the salt-tolerant cultivar. To achieve our goal, a two-factorial experiment with three sugarcane cultivars (CP-48, CP-57, CP-69) and two salinity levels (0 and 8 ds/m) was conducted. One-way ANOVA indicated that there was a significant difference between miRNAs and targeted gene expression. The highest reduction of miRNAs expression was occurred in miR160 while the lower one was happening in miR1432. The data also indicated that the higher and the lowest of targeted genes were in miR160 and miR393 respectively. Among studied cultivars, the CP-57 showed poor performance while CP-69 expresses a superior tolerance to salt stress. Taken together, these results suggested that the screening of well adapted cultivars under salt conditions would be appropriate solutions to combat salinity stress in saline lands.

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Specific Downregulation of the Bacterial-Type PEPC Gene by Artificial MicroRNA Improves Salt Tolerance in Arabidopsis

Cited by 18 publications

References 38 publications

MicroRNA‐Based Biotechnology for Plant Improvement

MicroRNA‐Based Biotechnology for Plant Improvement

Genome-Wide Identification and Analysis of the Phosphoenolpyruvate Carboxylase Gene Family in Suaeda aralocaspica, an Annual Halophyte With Single-Cellular C4 Anatomy

Comparative Analysis of Differentially Expressed miRNAs in Leaves of Three Sugarcane (Saacharum Officinarum L.) Cultivars During Salinity Stress

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