Specific downregulation of bcl-2 and xIAP by RNAi enhances the effects of chemotherapeutic agents in MCF-7 human breast cancer cells

Lima, Raquel T.; Martins, L. Miguel; Guimarães, José E.; Sambade, Clara; Vasconcelos, M. Helena

doi:10.1038/sj.cgt.7700706

Cited by 170 publications

(126 citation statements)

References 42 publications

Supporting

Mentioning

116

Contrasting

Order By: Relevance

“…This is consistent with recent reports that treatment of MCF-7 cells with Bcl-2 siRNA significantly reduced cell viability (50%-85%), but only slightly induced apoptosis (9%-11%), indicating that another cell death mechanism may be involved [56,67,68]. Interestingly, Bcl-2 also inhibited the beclin-1-de- npg pendent autophagy by binding to beclin 1.…”

Section: Discussionsupporting

confidence: 81%

Steroid receptor coactivator 3 regulates autophagy in breast cancer cells through macrophage migration inhibitory factor

et al. 2012

Cell Res

View full text Add to dashboard Cite

SRC-3/AIB1 (steroid receptor coactivator 3/amplified in breast cancer 1) is an authentic oncogene that contributes to the development of drug resistance and poor disease-free survival in cancer patients. Autophagy is also an important cell death mechanism that has tumor suppressor function. In this study, we identified macrophage migration inhibitory factor (MIF) as a novel target gene of SRC-3 and demonstrated its importance in cell survival. Specifically, we showed that MIF is a strong suppressor of autophagic cell death. We further showed that suppression of MIF, in turn, induced autophagic cell death, enhanced chemosensitivity and inhibited tumorigenesis in a xenograft mouse tumorigenesis model. Our study demonstrated that regulation of MIF expression and suppression of autophagic cell death is a potent mechanism by which SRC-3 contributes to increased chemoresistance and tumorigenicity.

show abstract

Section: Discussionsupporting

confidence: 81%

Steroid receptor coactivator 3 regulates autophagy in breast cancer cells through macrophage migration inhibitory factor

et al. 2012

Cell Res

View full text Add to dashboard Cite

show abstract

“…It enhanced the sensitivity to the anti-cancer agent imatinib mesylate, inhibited the expression of bcrabl, and promoted cell apoptosis. However, siRNA directing at bcr-abl homologous chromosomes could not enhance the sensitivity to imatinib mesylate and had no effect at all (Lima et al, 2004). The reason may be due to different degrees of down-regulation of bcr-Abl protein expression by the siRNA.…”

Section: Specific Mutation Ectopic Genementioning

confidence: 71%

RNA Interference for Tumor Therapy

Xia¹,

Ni²

2012

Biomedicine

View full text Add to dashboard Cite

“…RNA interference (antisense) of the anti-apoptotic Bcl-2 and XIAP sensitized cancerous cells to therapeutic agents. BI-1 antisense in MD-MBA-231 breast cancer cells resulted in spontaneous apoptosis, which was also the case in prostate cancer, indicating BI-1 is essential for survival in these cancers (Lima et al, 2004;Grzmil et al, 2006). However, BI-1 antisense is not immediately lethal in all breast cancer cell lines, presumably because Bcl-2 and Bcl-xL are overexpressed to varying degrees in 40-80% of human breast tumours (Grzmil et al, 2006).…”

Section: Bi-1 and Breast Cancermentioning

confidence: 78%