Specific DNA probes for the identification of the fish pathogen, Renibacterium salmoninarum

León, Gloria; Martínez, María Adela; Etchegaray, Jean‐Pierre; Vera, María; Figueroa, Jaime; Krauskopf, Manuel

doi:10.1007/bf00360876

Cited by 15 publications

(10 citation statements)

References 25 publications

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“…Strikingly, although BKD has been present in Chile for nearly 30 years, scientific studies are scarce and focus on BKD diagnosis (Leon et al . ) and molecularly cloning R. salmoninarum DNA fragments associated with bacterial internalization into CHSE cultured cells (Fiedler & Draxl ; Maulén et al . ).…”

Section: Renibacterium Salmoninarummentioning

confidence: 99%

Addressing viral and bacterial threats to salmon farming in Chile: historical contexts and perspectives for management and control

Figueroa

Cárcamo

Yáñez

et al. 2019

Reviews in Aquaculture

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The rapid growth of the salmon farming industry in Chile has led to the appearance of various viral, bacterial, parasitic, and fungal pathogens affecting farmed fish. The Chilean salmon industry has suffered several health crises associated with high fish mortalities, such as caused by the infectious salmon anaemia virus (2007) and harmful algal blooms (2016). In addition to these events, marine farms are continuously affected by outbreaks of harmful pathogens, including the bacteria Piscirickettsia salmonis and, most recently, a reappearance of Renibacterium salmoninarum, and the infectious pancreatic necrosis virus. These outbreaks have led to stricter regulations, but the salmon farming industry continues to struggle despite reforms. In addition to the aforementioned pathogens, which are a continuous threat, other apparently under control pathogens have also reappeared in recent years. In this review, we analyse the current state of knowledge on four of the main pathogens affecting salmon farming in Chile. The infectious pancreatic necrosis virus is relevant as it affects freshwater‐stage fish, with survivors then acting as carriers. The infectious salmon anaemia virus currently appears to be under control; however, P. salmonis and R. salmoninarum continue to be the cause for high mortalities in the Chilean aquaculture industry.

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Section: Renibacterium Salmoninarummentioning

confidence: 99%

Addressing viral and bacterial threats to salmon farming in Chile: historical contexts and perspectives for management and control

Figueroa

Cárcamo

Yáñez

et al. 2019

Reviews in Aquaculture

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“…Of relevance, a PCR was developed [60,61], which detected only 22 cells of R. salmoninarum; a sensitivity of 10 cells was reported by others [62]. Similarly, PCR detected only 10 2 colony forming units (CFU) of N. seriolae in yellowtail [63].…”

Section: Approaches To Characterizationmentioning

confidence: 99%

Taxonomy of bacterial fish pathogens

Austin

2011

Vet Res

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Bacterial taxonomy has progressed from reliance on highly artificial culture-dependent techniques involving the study of phenotype (including morphological, biochemical and physiological data) to the modern applications of molecular biology, most recently 16S rRNA gene sequencing, which gives an insight into evolutionary pathways (= phylogenetics). The latter is applicable to culture-independent approaches, and has led directly to the recognition of new uncultured bacterial groups, i.e. "Candidatus", which have been associated as the cause of some fish diseases, including rainbow trout summer enteritic syndrome. One immediate benefit is that 16S rRNA gene sequencing has led to increased confidence in the accuracy of names allocated to bacterial pathogens. This is in marked contrast to the previous dominance of phenotyping, and identifications, which have been subsequently challenged in the light of 16S rRNA gene sequencing. To date, there has been some fluidity over the names of bacterial fish pathogens, with some, for example Vibrio anguillarum, being divided into two separate entities (V. anguillarum and V. ordalii). Others have been combined, for example V. carchariae, V. harveyi and V. trachuri as V. harveyi. Confusion may result with some organisms recognized by more than one name; V. anguillarum was reclassified as Beneckea and Listonella, with Vibrio and Listonella persisting in the scientific literature. Notwithstanding, modern methods have permitted real progress in the understanding of the taxonomic relationships of many bacterial fish pathogens.

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“…Nucleic acid probes (Mattsson et al 1993, Leon et al 1994a, Hariharan et al 1995, and the polyrnerase chain reaction (PCR; Brown et al 1994, Le6n et al 1994b, McIntosh et al 1996, Pascho et al 1998 can be used to detect Renibactenum salmoninarum in tissues and fluids from infected fish. The PCR has the greatest potential for improving both the sensitivity and specificity of detection of R. salmoninarum by the enzymatic amplification cf a unique genome sequence (Saiki 1992).…”

Section: Introductionmentioning

confidence: 99%

Development of a nested polymerase chain reaction for amplification of a sequence of the p57 gene of Renibacterium salmoninarum that provides a highly sensitive method for detection of the bacterium in salmonid kidney

Chase¹,

Pascho²

1998

Dis. Aquat. Org.

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Nucleic acid-based assays have shown promise for diagnosing Renibacter~um salmoninarum in tissues and body fluids of salmonids. Development of a nested polymerase chain reaction (PCR) method to detect a 320 bp DNA segment of the gene encoding the p57 protein of R. salmoninarum is described. Whereas a conventional PCR for a 383 bp segment of the p57 gene reliably detected 1000 R. sahoninarum cells per reaction in kidney tissue, the nested PCR detected as few as 10 R. sdmoninarum per reaction in kidney tissue. Two DNA extraction methods for the nested PCR were compared and the correlation between replicate samples was generally higher in samples extracted by the QIAamp system compared with those extracted by the phenol/chloroform method. The specdlcity of the nested PCR was confirmed by testing DNA extracts of common bacterial fish pathogens and a panel of bacterial species reported to cause false-positi.ve reactions in the enzymelinked immunosorbent assay (ELISA) and the fluorescent antibody test (FAT) for R. salmoninarurn. Kidney samples from 74 naturally infected c h o o k salmon were examined by the nested PCR, the ELISA, and the FAT, and the detected prevalences of R. salmoninarum were 61, 47, and 43 %, respectively.

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Specific DNA probes for the identification of the fish pathogen, Renibacterium salmoninarum

Cited by 15 publications

References 25 publications

Addressing viral and bacterial threats to salmon farming in Chile: historical contexts and perspectives for management and control

Addressing viral and bacterial threats to salmon farming in Chile: historical contexts and perspectives for management and control

Taxonomy of bacterial fish pathogens

Development of a nested polymerase chain reaction for amplification of a sequence of the p57 gene of Renibacterium salmoninarum that provides a highly sensitive method for detection of the bacterium in salmonid kidney

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