Specific detection of Mycobacterium sp. genomic DNA using dual labeled gold nanoparticle based electrochemical biosensor

Chinnasamy, Thiruppathiraja; Senthilkumar, K.; Periyakaruppan, Adaikkappan; Santhosh, D. Jayakar; Alagar, Muthukaruppan

doi:10.1016/j.ab.2011.05.034

Cited by 96 publications

(42 citation statements)

References 22 publications

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“…Thiruppathiraja et al [58] developed a sandwich-based DNA biosensor for Mycobacterium sp. genomic DNA detection using dual labeled-AuNPs for amplification purposes.…”

Section: Electrochemical Genosensing Of Circulating Biomarkersmentioning

confidence: 99%

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Electrochemical Genosensing of Circulating Biomarkers

Campuzano

Yáñez‐Sedeño

Pingarrón

2017

Sensors

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Management and prognosis of diseases requires the measurement in non- or minimally invasively collected samples of specific circulating biomarkers, consisting of any measurable or observable factors in patients that indicate normal or disease-related biological processes or responses to therapy. Therefore, on-site, fast and accurate determination of these low abundance circulating biomarkers in scarcely treated body fluids is of great interest for health monitoring and biological applications. In this field, electrochemical DNA sensors (or genosensors) have demonstrated to be interesting alternatives to more complex conventional strategies. Currently, electrochemical genosensors are considered very promising analytical tools for this purpose due to their fast response, low cost, high sensitivity, compatibility with microfabrication technology and simple operation mode which makes them compatible with point-of-care (POC) testing. In this review, the relevance and current challenges of the determination of circulating biomarkers related to relevant diseases (cancer, bacterial and viral infections and neurodegenerative diseases) are briefly discussed. An overview of the electrochemical nucleic acid–based strategies developed in the last five years for this purpose is given to show to both familiar and non-expert readers the great potential of these methodologies for circulating biomarker determination. After highlighting the main features of the reported electrochemical genosensing strategies through the critical discussion of selected examples, a conclusions section points out the still existing challenges and future directions in this field.

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“…Thiruppathiraja et al [58] developed a sandwich-based DNA biosensor for Mycobacterium sp. genomic DNA detection using dual labeled-AuNPs for amplification purposes.…”

Section: Electrochemical Genosensing Of Circulating Biomarkersmentioning

confidence: 99%

“…genomic DNA detection using a specific probe immobilized on an ITO electrode and AuNPs modified both with detector probes and the enzyme alkaline phosphatase (AP). Reprinted from [58] with permission.…”

Section: Figurementioning

confidence: 99%

Electrochemical Genosensing of Circulating Biomarkers

Campuzano

Yáñez‐Sedeño

Pingarrón

2017

Sensors

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“…Duman et al (2009) demonstrated activity of biosensor developed in combination with gold nanoparticles, which is also used for the detection of M. tuberculosis. Similarly, Thiruppathiraja et al (2011) developed DNA electrochemical biosensor using gold nanoparticles for detection of genomic DNA of Mycobacterium sp. Gajendiran et al (2014) developed a gold conjugated poly(lactic-co-glycolic acid)-polyethylene glycol (PEG)-succinic anhydride (SA)-polyethylene glycol (PEG)-poly(lactic-co-glycolic acid) (i.e.…”

Section: Gold Nanoparticlesmentioning

confidence: 99%

Fungi as an efficient mycosystem for the synthesis of metal nanoparticles: progress and key aspects of research

et al. 2015

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Nanotechnology is an emerging cutting-edge technology, which involves interdisciplinary subjects, such as physics, chemistry, biology, material science and medicine. Different methods for the synthesis of nanoparticles have been discussed here. Although physical and chemical methods have been successfully used to synthesize nanoparticles, the use of hazardous chemicals and synthesis at high temperature is a matter of concern. Hence, there is a necessity to develop eco-friendly techniques for the synthesis of nanoparticles. Biosynthesis of nanoparticles by fungi, bacteria, actinomycetes, lichen and viruses have been reported eco-friendly. Moreover, the fungal system has emerged as an efficient system for nanoparticle synthesis as fungi possess distinctive characters including high wall binding capacity, easy to culture and simpler biomass handling, etc. In this review, we have discussed fungi as an important tool for the fabrication of nanoparticles. In addition, methods and mechanism for synthesis of nanoparticles and its potential applications have also been discussed.

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“…Further signal augmentation was attained via introduction of PCR amplification and the detection limit set at 0.5 ng/mL for Cd 2+ and 50 pg/mL for Pb 2+ . Also using an electrochemical approach, it was possible to directly detect M. tuberculosis DNA with a detection limit of 1.25 ng/mL [79]. Firstly, AuNP are dual-labeled with a complementary sequence of target DNA and an enzyme alkaline phosphatase.…”

Section: Electrochemical Detection Of Dna/rna Targets Using Aunpsmentioning

confidence: 99%

Gold Nanoparticles for DNA/RNA-Based Diagnostics

Franco¹,

Pedrosa²,

Carlos³

et al. 2015

Handbook of Nanoparticles

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The remarkable physicochemical properties of gold nanoparticles (AuNPs) have prompted development in exploring biomolecular interactions with AuNPs-containing systems, pursuing biomedical applications in diagnostics. Among these applications, AuNPs have been remarkably useful for the development of DNA/RNA detection and characterization systems for diagnostics, including systems suitable for point of need. Here, emphasis will be on available molecular detection schemes of relevant pathogens and their molecular characterization, genomic sequences associated with medical conditions (including cancer), mutation and polymorphism identification, and the quantification of gene expression.

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Specific detection of Mycobacterium sp. genomic DNA using dual labeled gold nanoparticle based electrochemical biosensor

Cited by 96 publications

References 22 publications

Electrochemical Genosensing of Circulating Biomarkers

Electrochemical Genosensing of Circulating Biomarkers

Fungi as an efficient mycosystem for the synthesis of metal nanoparticles: progress and key aspects of research

Gold Nanoparticles for DNA/RNA-Based Diagnostics

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