Specific Changes of Chromatin Structure in Nuclei of Normal Epithelium Adjacent to Laryngeal Squamous Cell Carcinoma

Dreyer, Thomas; I, Knoblauch; Garner, David M.; Doudkine, Alexei; MacAulay, Calum; Palcic, Branko; Popella, Christian

doi:10.1155/2000/461695

Cited by 4 publications

(3 citation statements)

References 25 publications

(26 reference statements)

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“…Changes in nuclear texture (chromatin features) have been shown in carcinomas from several different tissues, including colon, breast, larynx, and cervix (26–30). These features are present not only in malignant cells, but also in adjacent histologically normal cells.…”

Section: Discussionmentioning

confidence: 99%

Human papillomavirus‐related cellular changes measured by cytometric analysis of DNA ploidy and chromatin texture

Scheurer

Guillaud

Tortolero‐Luna

et al. 2007

Cytometry Part B Clinical

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Background: Image cytometry has provided two highly sensitive markers for the identification of the malignant potential of squamous lesions. Aneuploidy and chromatin texture have been investigated as quantitative measures of nuclear damage in premalignant lesions and carcinoma. Real-time PCR methods have evolved to yield highly specific measurements of mRNA expression in very sparse cellular samples.Methods: Human papillomavirus (HPV) 16 and 18 E7 mRNA expression was measured using quantitative RT-PCR. DNA index and chromatin measures were taken from image cytology samples. The chromatin features, through discriminant analysis, were aggregated into a score, and both measurements were related to mRNA expression.Results: mRNA level and DNA index show an increasing trend over increasing histological grades. However, DNA index and chromatin score were not correlated to mRNA levels in these samples. Chromatin score differed by mRNA type found with HPV 18 infected samples having a higher score than those with HPV 16. Samples infected with HPV 16 and HPV 18 had even higher chromatin scores.Conclusions: DNA index and chromatin score were not directly correlated with mRNA levels. However both mRNA and DNA index were related to histological grade, and chromatin score was associated with HPV type. Therefore, DNA index and mRNA levels could be independent predictors of cervical dysplasia, and chromatin score could be related to the viral integration process in cells infected with HPV 18 or dual infections. q 2007 Clinical Cytometry Society

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Section: Discussionmentioning

confidence: 99%

Human papillomavirus‐related cellular changes measured by cytometric analysis of DNA ploidy and chromatin texture

Scheurer

Guillaud

Tortolero‐Luna

et al. 2007

Cytometry Part B Clinical

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“…An even more serious argument in favor of estimating cell-level CC is a dimensionality reduction (e.g., by means of factor or cluster analysis). Modern programs for image analysis usually have tens of measurable parameters [1], and the maximum number of karyometric features (including textural) makes up to several hundreds to date [2,3]. Since it is often impossible to take correspondingly larger samples of patients, a low sample-feature ratio ensues.…”

Section: Discussionmentioning

confidence: 99%

Application of Multilevel Models to Morphometric Data. Part 2. Correlations

Tsybrovskyy

Berghold

2003

Analytical Cellular Pathology

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Multilevel organization of morphometric data (cells are “nested” within patients) requires special methods for studying correlations between karyometric features. The most distinct feature of these methods is that separate correlation (covariance) matrices are produced for every level in the hierarchy. In karyometric research, the cell‐level (i.e., within‐tumor) correlations seem to be of major interest. Beside their biological importance, these correlation coefficients (CC) are compulsory when dimensionality reduction is required. Using MLwiN, a dedicated program for multilevel modeling, we show how to use multivariate multilevel models (MMM) to obtain and interpret CC in each of the levels. A comparison with two usual, “single‐level” statistics shows that MMM represent the only way to obtain correct cell‐level correlation coefficients. The summary statistics method (take average values across each patient) produces patient‐level CC only, and the “pooling” method (merge all cells together and ignore patients as units of analysis) yields incorrect CC at all. We conclude that multilevel modeling is an indispensable tool for studying correlations between morphometric variables.

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“…A análise computacional de imagens microscópicas digitalizadas é uma técnica objetiva e reproduzível para o diagnóstico de caracteristicas celulares, e especialmente nucleares (15,16). Nesse contexto, a análise da textura nuclear permitiu diagnosticar neoplasias, estabelecendo as diferenças dos tecidos normais para os neoplásicos (17,18,19,(20)(21)(22). As mudanças na textura nuclear dos tecidos analisados, além de mudanças no conteúdo do DNA, puderam refletir a remodelação da cromatina causada por determinadas drogas como 17-β-estradiol e ácido valpróico (AVP), caracterizando a transformação neoplásica (16,23,24).…”

Section: A Textura Nuclear E Análise De Imagensunclassified

Análise dos parâmetros morfométricos e textura da cromatina dos mieloblastos nas síndromes mielodisplásicas

Vido¹

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vi AGRADECIMENTOSÀ Deus por tornar meu sonho possível e por ter proporcionado o encontro de pessoas especiais em meu caminho. À Profª Dra. Irene Lorand-Metze, que me aceitou como aluna e teve toda paciência para me orientar com muita competência. Pela amizade e confiança que construímos durante esses anos.Ao Prof. Dr. Konradim Metze pela colaboração e apoio na realização deste trabalho.Ao Prof. Dr. Randall Luis Adam pela ajuda em minhas inúmeras dúvidas durante minha pesquisa. Ao Dr. Eduardo I. Matsuda do Centro Infantil Boldrini, pela ajuda com minhas dúvidas sobre síndromes mielodisplásicas, pela ajuda com a identificação de células, pelo carinho, atenção e amizade.À minha mãe Rosa Elena e aos meus avós, Elena e Ramiro que sempre me orientaram e me aconselharam em todas as minha decisões.Ao meu namorado Alex, que sempre me apoiou e me deu forças em momentos de estresse.À minha tia Mary pelas várias caronas ao Hemocentro para eu realizar meu estudo.À bióloga Mônica A. Falconi pela ajuda no Laboratório de Hematologia do Hemocentro.À secretária do Hemocentro Nicete Romano pela sua competência e simpatia.À secretária do curso de Fisiopatologia Médica, Salete Gobbi, pela ajuda em todas as minhas dúvidas, por sua competência e por ter sido muito solicita quando precisei.Aos funcionários do Apoio didático do Hemocentro, Arlete e Leonardo pela ajuda com a formatação da minha tese.vii "Mesmo quando estivermos fracos demais e não nos reste nenhuma fé, Ele continua fiel para conosco e nos ajudará, pois não pode repudiar-nos, porque somos parte dele mesmo. E Ele sempre cumprirá suas promessas a nós." 2 Tm 2:13 "Eu aprendi muito mais com os meus erros do que com meus acertos.

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Specific Changes of Chromatin Structure in Nuclei of Normal Epithelium Adjacent to Laryngeal Squamous Cell Carcinoma

Cited by 4 publications

References 25 publications

Human papillomavirus‐related cellular changes measured by cytometric analysis of DNA ploidy and chromatin texture

Human papillomavirus‐related cellular changes measured by cytometric analysis of DNA ploidy and chromatin texture

Application of Multilevel Models to Morphometric Data. Part 2. Correlations

Análise dos parâmetros morfométricos e textura da cromatina dos mieloblastos nas síndromes mielodisplásicas

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