Specific biomolecule corona is associated with ring-shaped organization of silver nanoparticles in cells

Drescher, Daniela; Guttmann, Peter; Büchner, Tina; Werner, Stephan; Laube, Gregor; Hornemann, Andrea; Tarek, Basel; Schneider, Gerd; Kneipp, Janina

doi:10.1039/c3nr02129g

Cited by 51 publications

(90 citation statements)

References 52 publications

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“…Within the cell, the Ag-NPs formed aggregates in the cytoplasm and did not reach mitochondria or the nucleus. The formation of nanoparticle clusters within the cytoplasma of cells was already reported by different groups [3,24,25] and various studies have suggested an endocytotic uptake mechanism for Ag-NP [3,26]. Whether the Ag-NPs reach the nucleus often depends on the NP size, as the diameter of the nuclear pores is about 20-50 nm depending on the cell type [27,28].…”

Section: Inflammatory Reaction In Pbcecmentioning

confidence: 92%

The Influence of Silver Nanoparticles on the Blood-Brain and the Blood-Cerebrospinal Fluid Barrier in vitro

Cramer¹,

Tacke²,

Bornhorst³

et al. 2014

J Nanomed Nanotechnol

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The use of silver nanoparticles in medical and consumer products such as wound dressings, clothing and cosmetic has increased significantly in recent years. Still, the influence of these particles on our health and especially on our brain, has not been examined adequately up to now. We studied the influence of AgEO-(Ethylene Oxide) and AgCitrate-Nanoparticles (NPs) on the protective barriers of the brain, namely the blood-brain barrier (BBB) and the blood-cerebrospinal fluid (blood-CSF) barrier in vitro. The NPs toxicity was evaluated by examining changes in membrane integrity, cell morphology, barrier properties, oxidative stress and inflammatory reactions. AgNPs decreased cell viability, disturbed barrier integrity and tight junctions and triggered oxidative stress and DNA strand breaks. However, all mentioned effects were, at least partly, suppressed by a Citrate-coating and were most pronounced in the cells of the BBB as compared to the epithelial cells representing the blood-CSF barrier. AgEO-but not AgCitrate-NPs also triggered an inflammatory reaction in porcine brain capillary endothelial cells (PBCEC), which represent the BBB. Our data indicate that AgNPs may cause adverse effects within the barriers of the brain, but their toxicity can be reduced by choosing an appropriate coating material.

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Section: Inflammatory Reaction In Pbcecmentioning

confidence: 92%

The Influence of Silver Nanoparticles on the Blood-Brain and the Blood-Cerebrospinal Fluid Barrier in vitro

Cramer¹,

Tacke²,

Bornhorst³

et al. 2014

J Nanomed Nanotechnol

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“…Such junctions give rise to local field "hotspots" which are the source of the SERS effect in many studies, and are typically excited by source wavelengths of >600nm. For example, Drescher et al have demonstrated that the acidic environment of endosomes causes nanoparticle aggregation into dimers and trimers leading to an increased SERS effect at 785 nm 16 . Bonifacio et al have demonstrated that negligible SERS effects are observable in human serum samples, in which the serum proteins form a protein corona 33,34 , which coats the nanoparticles and prevents them from aggregating and forming hotspots, whereas, once the proteins are filtered out, strong and reproducible SERS signals can be recorded, again using 785 nm as source 35 .…”

Section: Discussionmentioning

confidence: 99%

“…Immediately after the addition was complete, the mixture was brought to the boil and maintained at this temperature for ~30 min. In order to evaluate the effect of aggregation on SERS from the gold nanospheres, acidic conditions were employed to induce nanoparticle aggregation 16 . HCl at a concentration of 1 M was added dropwise to aqueous suspensions of 150 nm nanospheres and the pH value was adjusted between 7 to 4.…”

Section: Methodsmentioning

confidence: 99%

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Surface enhanced Raman scattering with gold nanoparticles: effect of particle shape

et al. 2014

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The dependence of the Surface Enhanced Raman Scattering (SERS) by gold nanoparticles on their shape is examined using the organic dye, rhodamine 6G (R6G) as probe molecule. SERS has been explored extensively for applications in sensing and imaging, but the design and optimisation of efficient substrates is still challenging. In order to understand and optimise the SERS process in nanoparticles, gold nanospheres and their aggregates, nanotriangles, and nanostars of similar dimensions were synthesised and characterised according to their average size, zeta potential and UV/visible absorption. SERS from R6G was negligible for unaggregated nanospheres at 532 nm, close to the maximum of the surface plasmon resonance (SPR) at 560 nm. Upon aggregation of the nanospheres, the SPR shifts to ~660 nm, attributable to local surface plasmon "hotspots" between the spheres, and the SERS signal of R6G is significantly increased, at 785 nm. In monodisperse gold nanotriangles, the SPR is located at ~800 nm, and significant SERS of R6G is observed using 785 nm as source, as is the case for gold nanostars, which exhibit a double SPR with maxima at ~600 nm and ~785 nm, attributable to the core sphere and vertices of the structures, respectively. In suspensions of equal nanoparticle and dye concentration, the SERS effect increases as nanospheres

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“…The cells were incubated with such a mixture for 3 h. When both SEHRS labels were applied, the cells were incubated with 2-NAT SEHRS labels in culture medium for 3 h, and after washing with PBS, they were incubated with pMBA SEHRS labels in the culture medium, also for 3 h. After incubation, the cells were washed thoroughly with PBS buffer, in which they were also kept during the measurements. We have investigated the cytotoxicity of silver nanoparticles 33 and of the reporter molecules 11 in similar experiments, and found that no toxicity was associated under these experimental conditions with either of them, specifically with the concentrations used here.…”

Section: Application Of Sehrs Labels In Cellsmentioning

confidence: 99%

Surface-enhanced hyper Raman hyperspectral imaging and probing in animal cells

et al. 2017

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scattering, that is, spontaneous, two-photon excited Raman scattering, of organic molecules becomes strong when it occurs as surface-enhanced hyper Raman scattering (SEHRS), in the proximity of plasmonic nanostructures. Its advantages over one-photon excited surface-enhanced Raman scattering (SERS) include complementary vibrational information resulting from different selection rules, probing of very small focal volumes, and beneficial excitation with long wavelengths. Here, imaging of macrophage cells by SEHRS is demonstrated, using SEHRS labels consisting of silver nanoparticles and two different molecules, 2-naphthalenethiol and para-mercaptobenzoic acid, that are excited offresonance. The vibrational signatures of the molecules are discriminated using hyperspectral analysis and provide information about the subcellular localization of the SEHRS probes. The SEHRS based hyperspectral imaging approach presented here uses principal component analysis (PCA) to localize the reporter molecules inside the cells and is augmented by hierarchical cluster analysis (HCA). The high sensitivity of SEHRS spectra with respect to small environmental changes can be utilized for mapping of physiological parameters in the endosomal system of the cells. This is illustrated by discussing the spatial distribution of endosomes of varying pH inside the cytosol.

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Specific biomolecule corona is associated with ring-shaped organization of silver nanoparticles in cells

Cited by 51 publications

References 52 publications

The Influence of Silver Nanoparticles on the Blood-Brain and the Blood-Cerebrospinal Fluid Barrier in vitro

The Influence of Silver Nanoparticles on the Blood-Brain and the Blood-Cerebrospinal Fluid Barrier in vitro

Surface enhanced Raman scattering with gold nanoparticles: effect of particle shape

Surface-enhanced hyper Raman hyperspectral imaging and probing in animal cells

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