Specific Binding Sites for Triiodothyronine in the Plasma Membrane of Rat Thymocytes

Segal, Jacob; Ingbar, Sidney H.

doi:10.1172/jci110703

Cited by 93 publications

(27 citation statements)

References 31 publications

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“…This effect of T3 is evident within 20 min after the addition of the hormones; it is independent of new protein synthesis, is calcium-dependent, and is mediated by an increase in cellular cyclic AMP (cAMP) concentration secondary to an increase in adenylate cyclase activity. On the basis of these and other findings, we have suggested that this action of T3 is mediated at the level of the thymocyte plasma membrane, and consonant with this is our demonstration of the presence of specific, saturable binding sites for T3 in highly purified thymocyte plasma membrane preparations (6).…”

Section: Introductionsupporting

confidence: 85%

In vivo stimulation of sugar uptake in rat thymocytes. An extranuclear action of 3,5,3'-triiodothyronine.

Segal¹,

Ingbar²

1985

J. Clin. Invest.

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In previous studies we have demonstrated that 3,5,3'-triiodothyronine (T3) in vitro produces a prompt increase in the uptake of the sugar analogue 2-deoxyglucose (2-DG) by freshly isolated rat thymocytes. This effect is prompt, being evident at 20 min after addition of T3, is independent of new protein synthesis, and can be elicited by physiologic concentrations of the hormone. In the present studies, we have sought to determine whether physiologic doses of T3 are capable of inducing an increase in 2-DG uptake in the thymocytes of the living animal. Therefore, 26-28-d-old female rats were injected with increasing doses of i.v. T3, followed 60 min later by 3H-labeled 2-DG. 30 min later, animals were killed, thymocytes were isolated, and their 3H content determined. Uptake of PH12-DG was increased by T3 in a dose-dependent manner. The lowest effective dose was 10 ng/ 100 g of body weight (30% above control) and the maximally effective dose 1 gg/100 g of body weight (116% above control). The effect of T3 was independent of new protein synthesis in that it was not blocked by a dose of cycloheximide that inhibited the incorporation of 13Hlleucine into thymocyte protein by 92- These studies indicate that T3 in physiologic doses acts in vivo to increase the uptake of sugar by rat thymocytes by a mechanism that is extranuclear in origin, in that it is independent of new protein synthesis. The findings support the conclusion that the previously demonstrated effects of T3 on thymocyte sugar uptake in vitro, which seem clearly to be mediated at the level of the plasma membrane, have physiologic relevance. IntroductionWe have previously demonstrated that 3,5,3'-triiodothyronine (T3)' increases the uptake ofthe glucose analogue 2-deoxyglucose (2-DG) in isolated rat thymocytes in vitro (1-5). This effect of T3 is evident within 20 min after the addition of the hormones;

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Section: Introductionsupporting

confidence: 85%

In vivo stimulation of sugar uptake in rat thymocytes. An extranuclear action of 3,5,3'-triiodothyronine.

Segal¹,

Ingbar²

1985

J. Clin. Invest.

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“…Indeed, low plasma lipid levels, tachycardia, atrial arrhythmias, heart failure, muscle weakness and wasting are commonly found in hyperthyroid animals. Plasma membrane [89], endoplasmic reticulum [90] and mitochondria [91] have been considered as potential cellular sites of action of thyroid hormone. However, it is now generally accepted that most of the actions of thyroid hormone results from influences on trascription of T3-responsive genes, which are mediated through nuclear thyroid hormone receptors [92].…”

Section: Oxidative Stress In Experimental Hyperthyroidism and Hypothymentioning

confidence: 99%

Oxidative Stress and Antioxidant Status in Hypo- and Hyperthyroidism

Petrulea¹,

Muresan²,

Duncea³

2012

Antioxidant Enzyme

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“…In-422 P. Venditti and S. Di Meo Hyperthyroidism and oxidative stress deed, low plasma lipid levels, tachycardia, atrial arrhythmias, heart failure, muscle weakness and wasting are commonly found in hyperthyroid animals. Plasma membrane [117], endoplasmic reticulum [118] and mitochondria [119] have been considered as potential cellular sites of action of thyroid hormone. However, it is now generally accepted that most of the actions of thyroid hormone results from influences on trascription of T 3 -responsive genes, which are mediated through nuclear thyroid hormone receptors [120].…”

Section: Oxidative Stress-linked Dysfunction Of Hyperthyroid Tissuesmentioning

confidence: 99%

Thyroid hormone-induced oxidative stress

Venditti

Meo

2006

Cell. Mol. Life Sci.

337

230

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Hypermetabolic state in hyperthyroidism is associated with tissue oxidative injury. Available data indicate that hyperthyroid tissues exhibit an increased ROS and RNS production. The increased mitochondrial ROS generation is a side effect of the enhanced level of electron carriers, by which hyperthyroid tissues increase their metabolic capacity. Investigations of antioxidant defence system have returned controversial results. Moreover, other thyroid hormone-linked biochemical changes increase tissue susceptibility to oxidative challenge, which exacerbates the injury and dysfunction they suffer under stressful conditions. Mitochondria, as a primary target for oxidative stress, might account for hyperthyroidism linked tissue dysfunction. This is consistent with the inverse relationship found between functional recovery of ischemic hyperthyroid hearts and mitochondrial oxidative damage and respiration impairment. However, thyroid hormone-activated mitochondrial mechanisms provide protection against excessive tissue dysfunction, including increased expression of uncoupling proteins, proteolytic enzymes and transcriptional coactivator PGC-1, and stimulate opening of permeability transition pores.

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Specific Binding Sites for Triiodothyronine in the Plasma Membrane of Rat Thymocytes

Abstract: A B S T R A C T As a prerequisite to studies of whether the plasma membrane of the rat thymocyte contains specific, saturable binding sites for the thyroid hormone 3,5,3'-triiodothyronine (T3), a method was developed for the isolation of a plasma membrane fraction from these cells. As

Cited by 93 publications

References 31 publications

In vivo stimulation of sugar uptake in rat thymocytes. An extranuclear action of 3,5,3'-triiodothyronine.

In vivo stimulation of sugar uptake in rat thymocytes. An extranuclear action of 3,5,3'-triiodothyronine.

Oxidative Stress and Antioxidant Status in Hypo- and Hyperthyroidism

Thyroid hormone-induced oxidative stress

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