Specific binding of a 125I-secretoneurin analogue to a human monocytic cell line

Schneitler, Clemens; Kähler, Christian M.; Wiedermann, Christian J.; Hogue-Angeletti, Ruth A.; Fischer‐Colbrie, Reiner

doi:10.1016/s0165-5728(98)00012-5

Cited by 40 publications

(11 citation statements)

References 31 publications

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“…29 Functional and biochemical responses of ECs to SN, as observed in a previous 13 and the present study, implicate the presence of a putative SN receptor on these cells.…”

Section: Discussionsupporting

confidence: 84%

The Neuropeptide Secretoneurin Acts as a Direct Angiogenic Cytokine In Vitro and In Vivo

et al. 2004

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Background-Secretoneurin is an abundant neuropeptide of the central, peripheral, and autonomic nervous systems, located in nerve fibers characterized by a close interaction with blood vessels and known to stimulate endothelial cell migration. Methods and Results-We hypothesized that secretoneurin might act as an angiogenic cytokine and tested for these effects in vivo using a mouse cornea neovascularization model and in vitro by assessing capillary tube formation in a matrigel assay. In vivo, secretoneurin-induced neovasculature is characterized by a distinct pattern of arterial and venous vessels of large diameter and length. Immunohistochemical staining for CD-31 revealed endothelial lining of the inner surface of these vessels, and recruitment of ␣-smooth muscle actin-positive perivascular cells suggests vessel maturation. In vitro, secretoneurin-induced capillary tube formation was dose dependent and specific, confirming that effects of secretoneurin occur directly on endothelial cells. Secretoneurin also stimulated proliferation and exerted antiapoptotic effects on endothelial cells and activated intracellular phosphatidylinositol 3Ј kinase/Akt and mitogen-activated protein kinase pathways, as demonstrated by increased phosphorylation of Akt and extracellular signal-regulated kinase. Conclusions-These data show that secretoneurin represents a novel direct angiogenic cytokine and reiterate the coordinated relationship between nervous and vascular systems.

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“…29 Functional and biochemical responses of ECs to SN, as observed in a previous 13 and the present study, implicate the presence of a putative SN receptor on these cells.…”

Section: Discussionsupporting

confidence: 84%

The Neuropeptide Secretoneurin Acts as a Direct Angiogenic Cytokine In Vitro and In Vivo

et al. 2004

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“…These may include modulation of G-protein activation by regulators of Gprotein signaling and modification of phospholipase C. Phosphorylation of phospholipase Cß3 by both protein kinase A and protein kinase C has been demonstrated [72]. The presence of specific high affinity binding sites for SN was investigated on human monocytes [73] and on Mono Mac 6 cells, a monocytic cell line [74]. SN was iodinated with the Bolton-Hunter reagent and specific binding sites for 125 I-Bolton-Hunter-SN identified [74].…”

Section: Receptor Binding and Signalling Pathwaysmentioning

confidence: 99%

Secretoneurin: A New Player in Angiogenesis and Chemotaxis Linking Nerves, Blood Vessels and the Immune System

Fischer‐Colbrie

Kirchmair²,

Kähler³

et al. 2005

CPPS

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Secretoneurin (SN) represents a 33 amino acid neuropeptide, which is highly conserved between mammals, reptiles, birds, amphibians and fish. It is specifically expressed in endocrine, neuroendocrine and neuronal tissues. In brain, the pattern of SN expression is widespread and unique, partially overlapping with established neurotransmitters. ProSN, the precursor protein, also named secretogranin II, belongs to a class of proteins collectively called chromogranins. Changes in ProSN mRNA, which is significantly regulated by cell depolarisation, represent a useful marker for both rapid and long-lasting changes (positive and negative) of neuronal activity. Under pathophysiological conditions, especially following cellular hypoxia, SN expression can be induced in non-endocrine tissues like muscle cells, pneumocytes or tumor epithelial cells. Several biological effects were attributed to SN. SN releases dopamine from rat striatal slices in a dose dependent manner and influences neurite outgrowth in the developing cerebellum. It potently and specifically attracts monocytes, eosinophils and endothelial cells towards a concentration gradient and acts as an angiogenic cytokine comparable in potency to VEGF. Thus, SN contributes to neurogenic inflammation and might play a role in the (hypoxia-driven) induction of neo-vascularisation in ischemic diseases like peripheral or coronary artery disease, diabetic retinopathia, cerebral ischemia or in solid tumors. The signalling pathways of various biological effects have not been identified in detail, but most data point to a G-protein coupled receptor structure with respective associated intracellular events.

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“…In the central nervous system it may modulate neurotransmission since it stimulates DA release in the striatum (Agneter et al, 1995). Secretoneurin G-protein-linked receptors have been functionally characterized (Schneitler et al, 1998). The description of SN colocalized with NE in the SCG neurons (Klimaschewski et al, 1996b) prompted us to study this peptide in the rodent pineal gland (Simonneaux et al, 1997a).…”

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confidence: 99%

Generation of the Melatonin Endocrine Message in Mammals: A Review of the Complex Regulation of Melatonin Synthesis by Norepinephrine, Peptides, and Other Pineal Transmitters

2003

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Specific binding of a 125I-secretoneurin analogue to a human monocytic cell line

Cited by 40 publications

References 31 publications

The Neuropeptide Secretoneurin Acts as a Direct Angiogenic Cytokine In Vitro and In Vivo

The Neuropeptide Secretoneurin Acts as a Direct Angiogenic Cytokine In Vitro and In Vivo

Secretoneurin: A New Player in Angiogenesis and Chemotaxis Linking Nerves, Blood Vessels and the Immune System

Generation of the Melatonin Endocrine Message in Mammals: A Review of the Complex Regulation of Melatonin Synthesis by Norepinephrine, Peptides, and Other Pineal Transmitters

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