Specific antibodies to moesin, a membrane-cytoskeleton linker protein, are frequently detected in patients with acquired aplastic anemia

Takamatsu, Hiroyuki; Feng, Xingmin; Chuhjo, Tatsuya; Lu, Xuzhang; Sugimori, Chiharu; Okawa, Katsuya; Yamamoto, Miyuki; Iseki, Shoichi; Nakao, Shinji

doi:10.1182/blood-2006-07-036715

Cited by 47 publications

(29 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A previous study demonstrated the presence of anti-moesin Abs in 14 -34% of patients with rheumatoid arthritis (11,31), and a case-control study on AA conducted by the International Agranulocytosis and Aplastic Anemia Study Group revealed that a past history of rheumatoid arthritis is significantly associated with the later development of AA (32). The anti-moesin pAbs derived from patients with rheumatoid arthritis also enhanced TNF-␣ secretion from THP-1 cells (data not shown).…”

Section: Discussionmentioning

confidence: 99%

“…The specific bands were visualized by anti-moesin mAbs, pAbs from AA patients, or control human IgG pAbs from healthy individuals as described in a previous report (11). The expression levels of ␣-tubulin were determined as an internal control using Western blotting with anti-␣-tubulin mAbs (SigmaAldrich; no.…”

Section: Western Blottingmentioning

confidence: 99%

“…17-1128-0; GE Healthcare) and recombinant moesin protein (11) fixed on an agarose-gel column (1 ml, HiTrap NHS-activated HP, no. 17-0716-01; GE Healthcare) according to the manufacturer's instruction.…”

Section: Purification Of Anti-moesin Abs In the Sera Of Patients With Aamentioning

confidence: 99%

“…We previously demonstrated that Abs specific to moesin, a membrane-cytoskeleton linker protein in the cytoplasm, were detectable in approximately 40% of AA patients (11). Moesin is an intracellular protein that links the cell membrane and cytoskeleton, and mediates the formation of microtubules and cell adhesion sites as well as ruffling of the cell membrane (17).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Anti-Moesin Antibodies in the Serum of Patients with Aplastic Anemia Stimulate Peripheral Blood Mononuclear Cells to Secrete TNF-α and IFN-γ

Takamatsu

Espinoza

et al. 2009

The Journal of Immunology

Self Cite

View full text Add to dashboard Cite

Moesin is an intracellular protein that links the cell membrane and cytoskeleton, while also mediating the formation of microtubules and cell adhesion sites as well as ruffling of the cell membrane. To determine the roles of anti-moesin Abs derived from the serum of patients with aplastic anemia (AA) in the pathophysiology of bone marrow failure, we studied the expression of moesin on various blood cells and the effects of anti-moesin Abs on the moesin-expressing cells. The proteins recognized by anti-moesin mAbs were detectable on the surface of T cells, NK cells, and monocytes from healthy individuals as well as on THP-1 cells. The peptide mass fingerprinting of the THP-1 cell surface protein and the knock-down experiments using short hairpin RNA proved that the protein is moesin itself. Both the anti-moesin mAbs and the anti-moesin polyclonal Abs purified from the AA patients’ sera stimulated THP-1 cells and the PBMCs of healthy individuals and AA patients to secrete 60–80% as much TNF-α as did LPS 100 ng/ml. Although the polyclonal Abs induced IFN-γ secretion from the PBMCs of healthy individuals only when the PBMCs were prestimulated by anti-CD3 mAbs, the anti-moesin Abs were capable of inducing IFN-γ secretion from the PBMCs of AA patients by themselves. Anti-moesin Abs may therefore indirectly contribute to the suppression of hematopoiesis in AA patients by inducing myelosuppressive cytokines from immunocompetent cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Western Blottingmentioning

confidence: 99%

Section: Purification Of Anti-moesin Abs In the Sera Of Patients With Aamentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Anti-Moesin Antibodies in the Serum of Patients with Aplastic Anemia Stimulate Peripheral Blood Mononuclear Cells to Secrete TNF-α and IFN-γ

Takamatsu

Espinoza

et al. 2009

The Journal of Immunology

Self Cite

View full text Add to dashboard Cite

show abstract

“…49,50 Moreover, autoantibodies are frequently detected in patients with AA. 51,52 Perhaps dysregulations of B cells is related to aberrant B-cell activation. Previous research in patients with systemic lupus erythematosus demonstrated overlapping signatures in T-and B-cellexpressed miRNA which are differentially expressed.…”

Section: Discussionmentioning

confidence: 99%

Identification of novel microRNA signatures linked to acquired aplastic anemia

et al. 2015

View full text Add to dashboard Cite

show abstract

In‐depth proteomics approach of secretome to identify novel biomarker for sepsis in LPS‐stimulated endothelial cells

et al. 2015

View full text Add to dashboard Cite

Sepsis and septic shock, which are conditions triggered by infection, occur with high incidence in emergency departments and are among the most common causes of death in hospitalized patients worldwide. Therefore, the identification of sepsis biomarkers for the rapid diagnosis is a major goal for researchers in the field of critical care. Endothelial cells play a pivotal role in orchestrating the inflammatory response triggered by sepsis. In this study, we used proteomics to investigate the secretome of EA.hy926 endothelial cells following lipopolysaccharide (LPS) stimulation with 1 μg/mL LPS for 12 or 24 h. SILAC in cell cultures and an online 2D-LC-MS/MS system were used to analyze the secretome dynamics in response to LPS. We found that 22 of the 77 secreted proteins identified in both the presence and absence of LPS and that 19 of the secreted proteins were quantified more strongly after LPS treatment for 24 h than after treatment for 12 h. By Gene Ontology and KEGG pathway analyses, we found that proteins related to the regulation of the actin cytoskeleton showed the highest secretion response to LPS stimulation. Out of the 19 candidate proteins, we focused on moesin, which is involved in the function of endothelial cells, and confirmed its amount in cellular lysates and media taken from primary human umbilical vein endothelial cells treated with LPS. To our knowledge, this study provides the first in-depth analysis of the LPS-induced secretome in human endothelial cells, and we propose 19 new biomarker candidates for sepsis, including moesin.

show abstract

Specific antibodies to moesin, a membrane-cytoskeleton linker protein, are frequently detected in patients with acquired aplastic anemia

Cited by 47 publications

References 32 publications

Anti-Moesin Antibodies in the Serum of Patients with Aplastic Anemia Stimulate Peripheral Blood Mononuclear Cells to Secrete TNF-α and IFN-γ

Anti-Moesin Antibodies in the Serum of Patients with Aplastic Anemia Stimulate Peripheral Blood Mononuclear Cells to Secrete TNF-α and IFN-γ

Identification of novel microRNA signatures linked to acquired aplastic anemia

In‐depth proteomics approach of secretome to identify novel biomarker for sepsis in LPS‐stimulated endothelial cells

Contact Info

Product

Resources

About