Specific and Rapid SARS-CoV-2 Identification Based on LC-MS/MS Analysis

Schuster, Ofir; Zvi, Anat; Rosen, Osnat; Achdout, Hagit; Ben-Shmuel, Amir; Shifman, Ohad; Yitzhaki, Shmuel; Laskar, Orly; Feldberg, Liron

doi:10.1021/acsomega.0c04691

Cited by 17 publications

(27 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In another study by Schuster et al . a rapid SARS-CoV-2 identification was developed based on liquid chromatography-MS using six specific and sensitive SARS-CoV-2 peptide markers [ 46 ]. It is claimed that the assay can identify low concentration (10 4 PFU/ml) of SARS-CoV-2 in naïve nasopharyngeal swabs.…”

Section: Diagnosismentioning

confidence: 99%

“…Swab samples in the transfer media were directly applied to the MALDI-MS and results were analyzed by six different machine learning approaches and authors claimed to have results with high accuracy (93.9%), low false positive (7%), and false negative (5%) rates. In another study by Schuster et al a rapid SARS-CoV-2 identification was developed based on liquid chromatography-MS using six specific and sensitive SARS-CoV-2 peptide markers [46]. It is claimed that the assay can identify low concentration (10 4 PFU/ml) of SARS-CoV-2 in naïve nasopharyngeal swabs.…”

Section: Mass Spectrometry-based Technologymentioning

confidence: 99%

See 1 more Smart Citation

An update on novel approaches for diagnosis and treatment of SARS-CoV-2 infection

et al. 2021

View full text Add to dashboard Cite

The ongoing pandemic of coronavirus disease 2019 (COVID-19) has made a serious public health and economic crisis worldwide which united global efforts to develop rapid, precise, and cost-efficient diagnostics, vaccines, and therapeutics. Numerous multi-disciplinary studies and techniques have been designed to investigate and develop various approaches to help frontline health workers, policymakers, and populations to overcome the disease. While these techniques have been reviewed within individual disciplines, it is now timely to provide a cross-disciplinary overview of novel diagnostic and therapeutic approaches summarizing complementary efforts across multiple fields of research and technology. Accordingly, we reviewed and summarized various advanced novel approaches used for diagnosis and treatment of COVID-19 to help researchers across diverse disciplines on their prioritization of resources for research and development and to give them better a picture of the latest techniques. These include artificial intelligence, nano-based, CRISPR-based, and mass spectrometry technologies as well as neutralizing factors and traditional medicines. We also reviewed new approaches for vaccine development and developed a dashboard to provide frequent updates on the current and future approved vaccines.

show abstract

Section: Diagnosismentioning

confidence: 99%

Section: Mass Spectrometry-based Technologymentioning

confidence: 99%

An update on novel approaches for diagnosis and treatment of SARS-CoV-2 infection

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Recently, a study from our lab presented a rapid and reliable assay for SARS-CoV-2 identification based on specific markers derived from N and S SARS-CoV-2 proteins using high-resolution mass spectrometry. This assay enabled direct identification of 5 × 10 4 PFU/ml [ 29 ] SARS-CoV-2 in spiked buffer and an order of magnitude higher in NP specimens. These promising emerging MS-based methods are specific and rapid and may be beneficial as a research tool, or to supplement the diagnostic testing of COVID‐19.…”

Section: Introductionmentioning

confidence: 99%

Coupling immuno-magnetic capture with LC–MS/MS(MRM) as a sensitive, reliable, and specific assay for SARS-CoV-2 identification from clinical samples

et al. 2022

Self Cite

View full text Add to dashboard Cite

Graphical abstract Recently, numerous diagnostic approaches from different disciplines have been developed for SARS-CoV-2 diagnosis to monitor and control the COVID-19 pandemic. These include MS-based assays, which provide analytical information on viral proteins. However, their sensitivity is limited, estimated to be 5 × 10 4 PFU/ml in clinical samples. Here, we present a reliable, specific, and rapid method for the identification of SARS-CoV-2 from nasopharyngeal (NP) specimens, which combines virus capture followed by LC–MS/MS(MRM) analysis of unique peptide markers. The capture of SARS-CoV-2 from the challenging matrix, prior to its tryptic digestion, was accomplished by magnetic beads coated with polyclonal IgG-α-SARS-CoV-2 antibodies, enabling sample concentration while significantly reducing background noise interrupting with LC–MS analysis. A sensitive and specific LC–MS/MS(MRM) analysis method was developed for the identification of selected tryptic peptide markers. The combined assay, which resulted in S/N ratio enhancement, achieved an improved sensitivity of more than 10-fold compared with previously described MS methods. The assay was validated in 29 naive NP specimens, 19 samples were spiked with SARS-CoV-2 and 10 were used as negative controls. Finally, the assay was successfully applied to clinical NP samples ( n = 26) pre-determined as either positive or negative by RT-qPCR. This work describes for the first time a combined approach for immuno-magnetic viral isolation coupled with MS analysis. This method is highly reliable, specific, and sensitive; thus, it may potentially serve as a complementary assay to RT-qPCR, the gold standard test. This methodology can be applied to other viruses as well. Supplementary Information The online version contains supplementary material available at 10.1007/s00216-021-03831-5.

show abstract

“… 6 − 13 The available data support the potential of LC-MS/MS to achieve >90% sensitivity and nearly 100% specificity when using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) testing as the benchmark. 6 , 10 , 13 …”

Section: Introductionmentioning

confidence: 99%

Targeted Detection of SARS-CoV-2 Nucleocapsid Sequence Variants by Mass Spectrometric Analysis of Tryptic Peptides

et al. 2021

View full text Add to dashboard Cite

COVID-19 vaccines are becoming more widely available, but accurate and rapid testing remains a crucial tool for slowing the spread of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) virus. Although the quantitative reverse transcription-polymerase chain reaction (qRT-PCR) remains the most prevalent testing methodology, numerous tests have been developed that are predicated on detection of the SARS-CoV-2 nucleocapsid protein, including liquid chromatography-tandem mass spectrometry (LC-MS/MS) and immunoassay-based approaches. The continuing emergence of SARS-CoV-2 variants has complicated these approaches, as both qRT-PCR and antigen detection methods can be prone to missing viral variants. In this study, we describe several COVID-19 cases where we were unable to detect the expected peptide targets from clinical nasopharyngeal swabs. Whole genome sequencing revealed that single nucleotide polymorphisms in the gene encoding the viral nucleocapsid protein led to sequence variants that were not monitored in the targeted assay. Minor modifications to the LC-MS/MS method ensured detection of the variants of the target peptide. Additional nucleocapsid variants could be detected by performing the bottom-up proteomic analysis of whole viral genome-sequenced samples. This study demonstrates the importance of considering variants of SARS-CoV-2 in the assay design and highlights the flexibility of mass spectrometry-based approaches to detect variants as they evolve.

show abstract

Specific and Rapid SARS-CoV-2 Identification Based on LC-MS/MS Analysis

Cited by 17 publications

References 33 publications

An update on novel approaches for diagnosis and treatment of SARS-CoV-2 infection

An update on novel approaches for diagnosis and treatment of SARS-CoV-2 infection

Coupling immuno-magnetic capture with LC–MS/MS(MRM) as a sensitive, reliable, and specific assay for SARS-CoV-2 identification from clinical samples

Targeted Detection of SARS-CoV-2 Nucleocapsid Sequence Variants by Mass Spectrometric Analysis of Tryptic Peptides

Contact Info

Product

Resources

About