Specific and quantitative detection of PCR products from Clostridium piliforme, Helicobacter bilis, H. hepaticus, and mouse hepatitis virus infected mouse samples using a newly developed electrochemical DNA chip

Goto, Kazuo; Horiuchi, Hideki; Shinohara, Haruka; Motegi, Katsumi; Hashimoto, Koji; Hongo, Sadato; Gemma, Nobuhiro; Hayashimoto, Nobuhito; Itoh, Toshio; Takakura, Akira

doi:10.1016/j.mimet.2006.12.009

Cited by 25 publications

(17 citation statements)

References 19 publications

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“…The sequence of clone 8 matched 100% with that of the H. ganmani type strain. ten out of 14 clones (clones [5][6][7][8][9][10][11][12][13][14] were determined more than 1,400 bp sequences and these clones lacked any intervening sequences (iVs), as occurs in H. bilis [8] and H. typhlonius [9]. BLAST (blastn) searches between these 14 clones and the sequence of Helicobacter 16s rrna genes deposited in genbank were performed, and the results are shown in table 5. with the exception of clones 3 and 12, clones had the greatest homology with Helicobacter sp.…”

Section: Detection Of Multiple Helicobacter Spp In An Mit 01-6451-inmentioning

confidence: 99%

Prevalence of an Unidentified <i>Helicobacter</i> Species in Laboratory Mice and its Distribution in the Hepatobiliary System and Gastrointestinal Tract

Yamanaka

Arita

et al. 2013

Exp. Anim.

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An unidentified Helicobacter species, strain MIT 01-6451, was frequently detected in mice obtained from domestic commercial and academic institutions in Japan. To partially characterize this strain, its distributions in the gastrointestinal tract and hepatobiliary system of mice were investigated. In gastrointestinal tissues, this strain was detected in all cecum, colon, and feces samples tested, whereas fewer mice were positive in the ileum, jejunum, and duodenum. Interestingly, strain MIT 01-6451 was also detected in most stomach samples and in 33% of gallbladder samples. One mouse was found to be infected with multiple Helicobacter species. Fourteen copies of 16S rRNA genes were cloned from the tissues of this mouse. One had the highest level of sequence homology with H. canadensis, while 13 had the highest level of homology with the H. ganmani type strain or strain MIT 01-6451. Twelve of these 13 16S rRNA genes were mosaic sequences, being partially derived from H. ganmani and strain MIT 01-6451. These results suggest that H. ganmani and Helicobacter sp. MIT 01-6451 are prevalent in specific-pathogen-free mouse colonies in Japan and that lateral gene transfer probably occurs among Helicobacter species during coinfection.

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Section: Detection Of Multiple Helicobacter Spp In An Mit 01-6451-inmentioning

confidence: 99%

Prevalence of an Unidentified <i>Helicobacter</i> Species in Laboratory Mice and its Distribution in the Hepatobiliary System and Gastrointestinal Tract

Yamanaka

Arita

et al. 2013

Exp. Anim.

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“…Using the electrochemical DNA chip, a specific and quantitative detection of PCR products from 16S rRNA gene of H. bilis , H. hepaticus , and other pathogens was developed . An assay based on multiplex PCR‐denaturing gradient gel electrophoresis and pyrosequencing was developed by Nilsson et al.…”

Section: Discussionmentioning

confidence: 99%

Differentiation of five enterohepatic Helicobacter species by nested PCR with high‐resolution melting curve analysis

Rao

Zhu

et al. 2016

Helicobacter

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“…Excellent results for identification Most electrochemical DNA sensor arrays are used to detect products of the polymerase chain reaction (PCR), which increases the concentration of the sequence of interest in the sample. Goto et al [65] have reported a microfabricated DNA chip that they applied to both bacterial and viral nucleic acid detection. The chip consists of 40 gold working electrodes of 200 μm diameter along with common reference and counter electrodes.…”

Section: Electrochemical Nucleic Acid Arraysmentioning

confidence: 99%

Electrochemical Arrays for Bioassay Applications

Cortón

Mikkelsen

2016

Trends in Bioelectroanalysis

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This chapter presents a survey of developments and bioassay applications of individually addressable electrochemical arrays covering the past two decades and including over 90 references. Many different array designs have been fabricated, from macroscopic devices that have two to four sensing elements to ultramicroelectrode arrays containing as many as 12,000 individually addressable micrometer-sized working electrodes. Some inexpensively fabricated arrays are intended for single sample analysis and are disposable, while others are encapsulated for incorporation into reusable microfluidic systems. Devices and assays are grouped according to the types of modifications done to the sensing elements, and sections are included to consider unmodified elements as well as modifications with enzymes, antibodies or antigens, nucleic acids, in addition to viable cells and tissues. Some trends have been identified, and possible future directions are suggested.

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Specific and quantitative detection of PCR products from Clostridium piliforme, Helicobacter bilis, H. hepaticus, and mouse hepatitis virus infected mouse samples using a newly developed electrochemical DNA chip

Cited by 25 publications

References 19 publications

Prevalence of an Unidentified <i>Helicobacter</i> Species in Laboratory Mice and its Distribution in the Hepatobiliary System and Gastrointestinal Tract

Prevalence of an Unidentified <i>Helicobacter</i> Species in Laboratory Mice and its Distribution in the Hepatobiliary System and Gastrointestinal Tract

Differentiation of five enterohepatic Helicobacter species by nested PCR with high‐resolution melting curve analysis

Electrochemical Arrays for Bioassay Applications

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