Specific and nonspecific inhibition of adhesion of oral actinomyces and streptococci to erythrocytes and polystyrene by caseinoglycopeptide derivatives

Abstract

Various caseinoglycopeptide derivatives prepared from mammalian milk were evaluated as inhibitors of hemagglutinations mediated by Actinomyces viscosus Nyl, Streptococcus sanguis OMZ9, and, for comparative purposes, plant lectins from Arachis hypogaea and Bauhinia purpurea. It was found that recognition of the * Corresponding author.

In vitro modulation of oral bacterial adhesion to saliva‐coated hydroxyapatite beads by milk casein derivatives

“…These polypeptides of tnilk origin prevetited the adhesion of the test streptococci to S-HA, whereas the satne compounds did not interfere with the ability of A. viscosus Ny 1 to bind to S-HA. Thus, the inhibitory properties of these tnilk casein derivatives appear to be restricted to the streptococcal strains studied and are therefore clearly distinct from those of other inhibitors of microbial adhesion to nonbiological surfaces, such as BSA and PEG (39,43). The effect of these casein derivatives is not specifically mediated by phosphate groups or by terminal sialic acid residues.…”

“…In a previous study we have shown that, after desialylation, CGP behaved as a potent and specific inhibitor of hemagglutinations mediated by Actitwmyces viscosus Ny 1 (39). Also, both native and desialylated variants of CGP totally, but nonspecifically, prevented the adhesion of oral actinomyces and streptococci to polystyrene surfaces (39). Along with the in vivo results of Reynolds et al (41,42), these //; vitro data prompted us to investigate the eflects of bovine milk casein and CGP derivatives on the adhesion of test strains of/I.…”

“…Interestingly, theoligosaccharide chains borne by kappa-casein expose either terminal sialic acids (NeuNAc) or terminal /?-galactosides (/i-Gal), both carbohydrate residues being known to bind microbial lectins from various oral bacterial strains (8,25,26,34,37). In a previous study we have shown that, after desialylation, CGP behaved as a potent and specific inhibitor of hemagglutinations mediated by Actitwmyces viscosus Ny 1 (39). Also, both native and desialylated variants of CGP totally, but nonspecifically, prevented the adhesion of oral actinomyces and streptococci to polystyrene surfaces (39).…”

Inhibition of plasmid‐encoded adhesion of Yersinia enterocolitica by non‐immunoglobulin fraction of human milk

“…Other studies have demonstrated that the non-immunoglobulin fraction of HM may inhibit bacterial adhesion (1,2,3,8,10). This effect may, at least in part, be due to the presence of analogues of specific epithelial receptors for microorganisms (free oligosaccharides, glycoproteins) (1,3).…”

“…1. HM has also been shown to exert anti-adhesive effects on certain Escherichiu coli strains ( 3 , S), Streptococcus pneumoniae and Haemophilus influenzae (1,2), and oral Actinomyces and streptococci (10).…”