Specific amplification of gene encoding N-terminal region of catalase–peroxidase protein (KatG-N) for diagnosis of disseminated MAC disease in HIV patients

Background: Disseminated Mycobacterium avium complex (MAC) and Mycobacterium tuberculosis ( M. tuberculosis) infections have almost similar clinical presentations but require different therapeutic management. Materials & methods: A duplex PCR was designed based on the sequence variation between the genes encoding catalase-peroxidase (KatG) of M. avium complex and M. tuberculosis, so as to discriminate MAC, M. tuberculosis and mixed mycobacterial (MAC + M. tuberculosis) infections in HIV patients. Results: An accurate, single-step differential diagnosis of disseminated mycobacterial infections in HIV patients was achieved with specific detection of a single band each for M. avium (120 bp) and M. tuberculosis (90 bp) and two bands for the mixed (120 and 90 bp) infections. Conclusion: katG gene-based duplex PCR can facilitate quick differential diagnosis of disseminated MAC and M. tuberculosis infections in HIV patients.

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Differential Diagnosis of Disseminated Mycobacterium Avium and Mycobacterium Tuberculosis Infection in HIV Patients Using Duplex PCR

Sharma

Latawa

Wanchu

et al. 2021

Future Microbiol.

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Specific amplification of gene encoding N-terminal region of catalase–peroxidase protein (KatG-N) for diagnosis of disseminated MAC disease in HIV patients

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Differential Diagnosis of Disseminated Mycobacterium Avium and Mycobacterium Tuberculosis Infection in HIV Patients Using Duplex PCR

Differential Diagnosis of Disseminated Mycobacterium Avium and Mycobacterium Tuberculosis Infection in HIV Patients Using Duplex PCR

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