Specific age related signatures in Drosophila body parts transcriptome

Abstract

Background: During the last two decades progress in the genetics of aging in invertebrate models such as C. elegans and D. melanogaster has clearly demonstrated the existence of regulatory pathways that control the rate of aging in these organisms, such as the insulin-like pathway, the Jun kinase pathway and the Sir2 deacetylase pathway. Moreover, it was rapidly shown that some of these pathways are conserved from yeast to humans.

Evidence of neuroprotective effects of saffron and crocin in a Drosophila model of parkinsonism

“…Over the last decade, genome-wide expression analysis equipment such as microarray technology have been often functional to discover the machinery crucial for aging. Transcriptomic research has been conducted in invertebrate models such as Caenorhabditis elegans and Drosophila melanogaster (Girardot et al, 2006;Viñuela et al, 2010). Environmental effects, such as diet, are often recognized to exert effects on aging and considerable significance has been devoted towards exploring the impact of dietary and caloric restriction (Tatar, 2011;Uno et al, 2013;Zhu et al, 2014).…”

Drosophila melanogaster mitochondrial Hsp22: a role in resistance to oxidative stress, aging and the mitochondrial unfolding protein response

“…Indeed, hsp22 is one of the most up-regulated genes in tko 25 flies that have an insufficient mitochondrial translational capacity due to a mutation in mitoribosomal protein S12 (mRpS12) (Fernandez-Ayala et al 2010). Moreover, hsp22 together with l(2)efl (another sHSP from Drosophila), are the sHSPs genes showing the greatest induction upon oxidative stress (H 2 O 2 , paraquat, C99.5 % O 2 atmosphere (Girardot et al 2006;Gruenewald et al 2009;Hirano et al 2012;Landis et al 2004Landis et al , 2012.…”

Temporal and spatial transcriptional profiles of aging in Drosophila melanogaster

“…Previous genomic studies of aging used the whole body or body parts in invertebrates, including D. melanogaster (Golden and Melov 2004), and some of these studies only compared transcript levels of two ages, young and old, which missed valuable information regarding temporal and/or spatial expression patterns of aging. Girardot et al (2006) attempted to investigate spatial expression profiles of aging by comparing age-related changes among the whole body and two body parts, head and thorax, in D. melanogaster between two age-points, 3 and 40 d. They identified more than 1000 age-related genes in each body part but found that only a small percentage (<7%) of the agerelated genes were shared among the whole body and two body parts, and ∼18% of the age-related genes were common between head and thorax, despite the fact that these body parts share a number of similar tissue types, such as fat cells and muscles. The relative homogenous tissues used in our study offered an improved resolution for tissue comparison and showed much less overlaps of age-related genes (<10%) between any two tissues.…”

“…However, little is known about expression patterns of aging across different tissues or organs and how they interact with each other during aging. In all the previous genome-wide transcriptional studies on aging in invertebrates, including C. elegans and D. melanogaster, gene expression was monitored with the whole body or body parts, such as fly head and thorax (Zou et al 2000;Lund et al 2002;Pletcher et al 2002;Landis et al 2004;Wang et al 2004;Kim et al 2005;Girardot et al 2006). Many of these studies sampled only two age-points, e.g., young and old.…”