Specific adjustments in grapevine leaf proteome discriminating resistant and susceptible grapevine genotypes to Plasmopara viticola

Figueiredo, Andreia; Martins, Joana; Sebastiana, Mónica; Guerreiro, Ana; Silva, Ana; Matos, Ana Rita; Monteiro, Filipa; Pais, M. S.; Roepstorff, Peter; Coelho, Ana Varela

doi:10.1016/j.jprot.2016.10.012

Cited by 42 publications

(49 citation statements)

References 55 publications

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“…assimilation was noticed few hours after pathogen contact with its host, and such a decrease remained up to 12 days after P. viticola inoculation in resistant cultivars Solaris, Cabernet Blanc and Phoenix. This behavior could result from the downregulation of proteins related to photosynthesis and carbohydrate metabolism as previously reported in resistant grapevine cultivars (Figueiredo et al, 2017;Nascimento-Gavioli et al, 2017). A fast and massive transcription of signaling genes affected by P. viticola has also been reported on Rpv-bearing in grapevine cultivars (Fröbel et al, 2019).…”

Section: Discussionsupporting

confidence: 66%

Photosynthetic Cost Associated With Induced Defense to Plasmopara viticola in Grapevine

et al. 2020

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Downy mildew caused by Plasmopara viticola is one of the most destructive diseases of Vitis vinifera worldwide. Grapevine breeding programs have introgressed P. viticolaresistant traits into cultivated V. vinifera genotypes and launched interspecific hybrids with resistance against downy mildew. In general, pathogen infection affects primary metabolism, reduces plant growth and development and modifies the secondary metabolism toward defense responses, which are costly in terms of carbon production and utilization. The objective of this work was to evaluate the photosynthesis impairment by inducible defenses at the leaf level in V. vinifera cultivars resistant to P. viticola. Photosynthetic limitations imposed by P. viticola in susceptible and resistant grapevine cultivars were evaluated. Histochemical localization of hydrogen peroxide and superoxide and the activity of ascorbate peroxidase were assessed. Measurements of leaf gas exchange, chlorophyll fluorescence and the response of leaf CO 2 assimilation to increasing air CO 2 concentrations were taken, and photosynthetic limitations determined in cultivars Solaris (resistant) and Riesling (susceptible). The net photosynthetic rates were reduced (−25%) in inoculated Solaris plants even before the appearance of cell death-like hypersensitive reactions ("HR"). One day after "HR" visualization, the net photosynthetic rate of Solaris was reduced by 57% compared with healthy plants. A similar pattern was noticed in resistant Cabernet Blanc and Phoenix plants. While the susceptible cultivars did not show any variation in leaf gas exchange before the appearance of visual symptoms, drastic reductions in net photosynthetic rate and stomatal conductance were found in diseased plants 12 days after inoculation. Decreases in the maximum Rubisco carboxylation rate and photochemical impairment were noticed in Riesling after inoculation with P. viticola, which were not found in Solaris. Damage to the photochemical reactions of photosynthesis was likely associated with the oxidative burst found in resistant cultivars within the first 24 h after inoculation. Both chlorophyll degradation and stomatal closure were also noticed in the incompatible interaction. Taken together, our data clearly revealed that the defense response against P. viticola causes a photosynthetic cost to grapevines, which is not reversible even 12 days after the pathogen infection.

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Section: Discussionsupporting

confidence: 66%

Photosynthetic Cost Associated With Induced Defense to Plasmopara viticola in Grapevine

et al. 2020

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“…Differences in susceptibility were also seen between different species of Vitis, as earlier mentioned by several authors (Yu et al, 2012;Boso et al, 2014). Figueiredo et al (2017) explained that following inoculation increase of hydrogen peroxide levels, cellular redox regulation, establishment of ROS signalling and plant cell death seem to be key points differentiating the resistant genotype. Lipid associated signalling events, particularly related to jasmonates appear also to play a major role in the establishment of resistance.…”

Section: Gráfico De Distribuição Com Base No Número De Acessos De Sevmentioning

confidence: 61%

Susceptibility to downy mildew (Plasmopara viticola) and powdery mildew (Erysiphe necator) of differentVitiscultivars and genotypes

Atak¹,

Akkurt

Çelik

et al. 2017

Ciência Téc. Vitiv.

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SUMMARYTurkey has a very old history of viticulture and also homeland of the grapevine (Vitis spp.). Vitis cultivars belonging to different species are grown in almost every region in the country. However, particularly downy mildew and powdery mildew diseases affect the cultivars belonging to Vitis vinifera. In northern of Turkey Vitis labrusca and hybrids between V.vinifera and V.labrusca are rather common. V.labrusca cultivars or genotypes exhibit generally higher degree of resistance than V. vinifera cultivars. However, resistance level can vary from cultivar to cultivar and even from clone to clone within one cultivar. In this study, different Vitis hybrids and genotypes which exhibit different downy and powdery mildew susceptibility are compared for two years. Especially some V.labrusca hybrids and genotypes appeared resistance for both diseases. On the other hand, interspecific crosses and V.vinifera cultivars were found to be more susceptible. Using resistant lines as parent in later breeding activities, it could be possible grow high quality table cultivars with much fewer pesticide applications or possibly without them. RESUMOA Turquia tem uma longa história vitícola, sendo inclusivé um dos pontos de origem da videira (Vitis spp.). As diferentes espécies de Vitis são cultivadas em quase todas as regiões do país. Doenças como o míldio e oídio afetam em particular as videiras pertencentes à espécie V.vinifera. No Norte da Turquia, as espécies Vitis labrusca e híbridos entre V. vinifera e V. labrusca são bastante comuns. As cultivares/genótipos de V. labrusca exibem um grau de resistência geralmente mais elevado do que as cultivares de V. vinifera. No entanto o nível de resistência pode variar de cultivar para cultivar e até mesmo de clone para clone dentro de uma mesma cultivar. Neste estudo foram comparados diferentes híbridos de Vitis e genótipos com diferentes suscetibilidades ao míldio e ao oídio por um período de dois anos. Alguns híbridos e genótipos de V.labrusca apresentaram resistência a ambas as doenças. Por outro lado, os cruzamentos interespecíficos e as cultivares de V.vinifera foram identificados como sendo mais susceptíveis. Usando linhas resistentes como linha parental em atividades de melhoramento posteriores, poderia ser possível desenvolver cultivares para produção de vinhos de mesa de alta qualidade com menores exigências de aplicações de pesticidas ou possivelmente sem eles.

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“…Infection also slightly decreased chlorophyll content and SPAD, although chlorophyll uorescence did not appear to be affected. This biotroph has been shown to lower the photosynthetic rate (Jermini et al 2010;Figueiredo et al 2017) through the loss of chlorophyll, the downregulation of chlorophyll a/b binding protein, chlorophyll synthase and Rubisco and the upregulation of chlorophyllase (Gamm et al 2011). Just 2 days after inoculation, the chlorophyll losses recorded were still small, likely because the chlorophyll content only decreases within the infected lesion (Moriondo et al 2005;Gamm et al 2011), and might still have been insu cient to affect the chlorophyll uorescence (Cséfalvay et al 2009).…”

Section: Single Pathogen Stressmentioning

confidence: 99%

Responses to Drought Stress Modulate the Susceptibility to Plasmopara Viticola in Grapevine

Heyman

Chrysargyris

Demeestere

et al. 2020

Preprint

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Climate change will increase the occurrence of plants simultaneously suffering drought and pathogen stress. Although it is well-known that drought can alter the way plants respond to pathogens, the knowledge about the effect of concurrent drought and biotic stress in grapevine is scarce. This is especially true for Plasmopara viticola, the causal agent of grapevine downy mildew. This research addresses how vines with different drought tolerance respond to the challenge with P. viticola, drought stress or their combination, and how one stress affects the other. An artificial inoculation was performed on two cultivars, exposed to full or deficit irrigation, in the Mediterranean climate of Cyprus. In parallel, leaf disks from these plants were inoculated in controlled conditions. Leaves were sampled at an early infection stage to determine the influence of the single and combined stresses on oxidative parameters, chlorophyll and phytohormones. Under irrigation, the local Cypriot cultivar Xynisteri was more susceptible to P. viticola than the drought-sensitive Chardonnay. Drought stress increased their susceptibility in leaves inoculated in controlled conditions. Conversely, both cultivars showed resistance against P. viticola when inoculated in planta under continued deficit irrigation. Despite their resistance, the pathogen-associated responses in auxin, antioxidant enzyme activity and proline still occurred in these drought-stressed plants. Surprisingly, abscisic acid, rather than the generally implicated jasmonic and salicylic acid, seemed to play a prominent role in this resistance. The irrigation-dependent susceptibility highlights that the changing climate and the practices used to mitigate its effects, may have a profound impact on plant pathogens.

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Specific adjustments in grapevine leaf proteome discriminating resistant and susceptible grapevine genotypes to Plasmopara viticola

Cited by 42 publications

References 55 publications

Photosynthetic Cost Associated With Induced Defense to Plasmopara viticola in Grapevine

Photosynthetic Cost Associated With Induced Defense to Plasmopara viticola in Grapevine

Susceptibility to downy mildew (Plasmopara viticola) and powdery mildew (Erysiphe necator) of differentVitiscultivars and genotypes

Responses to Drought Stress Modulate the Susceptibility to Plasmopara Viticola in Grapevine

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