Specific Adhesion of 3T3-L1 Fibroblast onto Uridine-Containing Polystyrene

Synthesis of Polyethylene Glycol (PEG) Derivatives and PEGylated−Peptide Biopolymer Conjugates

“…There was no correlation between network surface hydrophilicity and adherent cell density. Some research groups also have the same finding. − However, the other research groups found that higher hydrophilicity correlated to higher cell adhesion. − We argue that there is actually no direct relationship between surface hydrophilicity and adherent cell density. Cellular function mediated by adsorbed proteins and substrate chemicophysical properties is a complex and dynamic interrelationship.…”

Correlation between Surface Properties of Polystyrene and Polylactide Materials and Fibroblast and Osteoblast Cell Line Behavior: A Critical Overview of the Literature

“…53,57,[91][92][93][94]58,60,65,[83][84][85]87,89 However, some articles also reported an increase in osteoblast and fibroblasts adhesion and proliferation for substrates with water contact angles over 90°(up to 120°) and below 30°(down to 15°). 52,55,56,59,80,86,96,108 The influence of well-defined chemical functional groups grafted on the surface was also well-documented in the literature to improve cell behavior. 27 More specifically, it was shown that surfaces containing oxygen (like −COOH groups) or nitrogen (like −NH 2 groups) promoted cell adhesion, proliferation, and viability.…”

“…above 90°) contact angles result in poor cell adhesion. More specifically for PS and PLA, our literature survey highlighted that the majority of modifications leading to cell interaction were corresponding to water contact angles ranging between 40° and 60° for osteoblasts ,− ,,, and between 40° and 70° for fibroblasts. ,,− ,,,,− ,, However, some articles also reported an increase in osteoblast and fibroblasts adhesion and proliferation for substrates with water contact angles over 90° (up to 120°) and below 30° (down to 15°). ,,,,,,, …”

“…Interestingly enough, thanks to a lower critical solution temperature of poly- N -isopropylacrylamide close to 37 °C, the authors succeeded to detach the cells by a decrease of temperature below 32 °C, thus without trypsinization (no quantitative results). Beside these covalent grafting approaches, Hatanaka et al succeeded to coat TCPS multiwells with poly­(uridine 5′- p -styrenesulfonate) using only a physical adsorption process . Compared to untreated multiwells, cell adhesion after 2.5 h of culture was increased by a factor 1.2 and 1.5 using 0.01 or 50 mg/mL of poly­(uridine 5′- p -styrenesulfonate), respectively.…”

“…Beside these covalent grafting approaches, Hatanaka et al succeeded to coat TCPS multiwells with poly(uridine 5′-p-styrenesulfonate) using only a physical adsorption process. 59 Compared to untreated multiwells, cell adhesion after 2.5 h of culture was increased by a factor 1.2 and 1.5 using 0.01 or 50 mg/mL of poly(uridine 5′-pstyrenesulfonate), respectively. Polyelectrolyte complexes made from polyethylenimine and heparin were deposed on TCPS dishes by Mao et al 60 Within these thin films, acid fibroblast growth factors were also incorporated.…”