Specific adherence of thymocytes to a thymic medullary epithelial cell line

Potworowski, Édouard F.; Thibodeau, Lise; Zelechowska, M

doi:10.1016/0165-2478(86)90132-x

Cited by 8 publications

(3 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We also showed that the putative ligand of TMF is a trypsinsensitive protein, and that if it is a glycoprotein. its polysaccharidc moiety was not involved in binding, corroborating previous work in which sugars could not block adherence [22].…”

Section: Discussionsupporting

confidence: 88%

Dynamics of Complex Formation between Thymocytes and Thymic Medullary Epithelial Cells

Hugo

Potworowski

1989

Scand J Immunol

View full text Add to dashboard Cite

Direct cell contact is an intrinsic part of several differentiation processes. A case in point is the formation of complexes between thymic lymphocytes and stromal cells, which are essential for T-cell maturation. The objective of the present work was to gain an insight into the mechanisms underlying the formation and dissociation of such lymphostromal complexes. Using an in vitro system, we show that the number of thymocytes adhering to a thymic medullary epithelial cell line (E-5) increases with time and reaches a plateau, after which some thymocytes spontaneously detach, while the rest remain attached to epithelium. The detached and forcibly removed thymocytes were analysed for their expression of L3T4 and Ly-2 antigens. The detached thymocytes showed a markedly lowered expression of both antigens. Both of these subtypes were shown to be totally refractory to form further complexes after a first encounter with E-5 cells. We also show that, once a mean adherence level of one thymocyte per E-5 cell was reached, (a) the level of further adherence increased exponentially, and (b) during this phase, binding of thymocytes to E-5 cells occurred in clusters, indicating pre-existing polarity or contact-induced polarization of the receptors at the E-5 cell surface.

show abstract

Section: Discussionsupporting

confidence: 88%

Dynamics of Complex Formation between Thymocytes and Thymic Medullary Epithelial Cells

Hugo

Potworowski

1989

Scand J Immunol

View full text Add to dashboard Cite

show abstract

“…TEM studies showed thymocytes beneath more superficial TS cells. Several previous reports demonstrated thymocyte attachment to thymus epithelial cell lines in vitro (Hiai et al, 1981(Hiai et al, , 1985Potworowski et al, 1986). Interestingly, some have reported that thymocytes appear to be ingested by thymus epithelial cells, as well as by macrophages.…”

Section: Discussionmentioning

confidence: 93%

“…Despite the heterogeneity of the stromal cells, macrophage-thymocyte and epithelial-thymocyte interactions can be clearly discriminated (Jason and Janeway, 1984;Jones, 1986). Thymocytes also adhere to murine thymus epithelial cell lines (Hiai et al, 1985;Potworowski et al, 1986). In addition, the number of epithelial cells that bind thymocytes increases with time in coculture (Hiai et al, 1985).…”

mentioning

confidence: 99%

Kinetic analysis of thymocyte attachment to thymus stromal cells in culture by using phase‐contrast and scanning electron microscopy

Larochelle

Jones

1989

Anat. Rec.

View full text Add to dashboard Cite

Direct cellular contact between thymocytes and thymus stromal cells within the thymus appears to contribute to the maturation of thymocytes. Thymocyte-stromal cell complexes, formed in vivo, have been isolated by others and postulated to play a role in T-cell differentiation. These previous studies have been hampered, however, by a time-consuming isolation procedure from which only small numbers of these complexes are recovered. We have examined a model to study thymocyte-stromal cell complexes in vitro in which thymocytes are added to primary cultures of thymus stromal cells. In the present study, we found that thymocytes were histotypically selective in their attachment to thymus stromal cells. We also investigated the kinetics of thymocyte attachment to these thymus stromal cells. Cultures were examined at selected time intervals from 5 min through 3 days of incubation. Thymocyte attachment to stromal cells was a biphasic interaction, with maximum surface attachment at 15 min of cocultivation, followed by migration of thymocytes into the cultures. Morphological studies were confirmed by using 3H-leucine-labeled thymocytes and liquid scintigraphy. With increased time in culture, thymocytes became amoeboid and migrated between the layers of stromal cells where thymocyte mitotic figures were seen at 4 and 8 hr. In some cases it appeared that stromal cells, which often grew two to three cell layers deep, played an active role in enclosing thymocytes within the cultures. Large numbers of viable thymocytes were observed in the cultures at 24 hr. The number of thymocytes then decreased progressively on days 2 and 3, when relatively few were found within the layers of the culture.

show abstract

Embryology of the Mammalian Thymus

Immunological Aspects of Neoplasia — The Role of the Thymus

View full text Add to dashboard Cite

Specific adherence of thymocytes to a thymic medullary epithelial cell line

Cited by 8 publications

References 5 publications

Dynamics of Complex Formation between Thymocytes and Thymic Medullary Epithelial Cells

Dynamics of Complex Formation between Thymocytes and Thymic Medullary Epithelial Cells

Kinetic analysis of thymocyte attachment to thymus stromal cells in culture by using phase‐contrast and scanning electron microscopy

Embryology of the Mammalian Thymus

Contact Info

Product

Resources

About