Specific adherence of Escherichia coli (strain RDEC-1) to membranous (M) cells of the Peyer's patch in Escherichia coli diarrhea in the rabbit.

Inman, Lindsey; Cantey, J. Robert

doi:10.1172/jci110737

Cited by 181 publications

(62 citation statements)

References 25 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lymphoid follicle epithelium from Peyer's patch tissues of rabbits challenged with ShDl2 strain bacteria at both 2 and 6 h postinoculation had no associated bacteria (2). Furthermore, the lymph follicle epithelium was intact and there was no infiltration of inflammatory cells.…”

Section: Resultsmentioning

confidence: 96%

“…The bacterium adheres to M cells of the Peyer's patch lymphoid follicle epithelium within a few hours postinoculation, causing acute inflammation as early as 12 h (2,3). The gut lumen is subsequently colonized and by 3-6 d postinoculation, RDEC-I strain bacteria can be seen adhering closely to the surfaces of ileal, cecal, and colonic absorptive epithelial cells that have been denuded of microvillus border (4).…”

mentioning

confidence: 99%

“…Antisera to the RDEC-I and the ShD 12 bacteria were prepared in rabbits by intravenous injection of bacteria according to procedures described elsewhere (1) buffer at room temperature for 3 h. The tissue was then prepared for thick and thin sectioning by embedment in Epon 812 (Electron Microscopy Sciences, Fort Washington, PA) as described previously (2). After postembedment staining with uranyl acetate and lead citrate, sections were examined at an accelerating voltage of 75 kV with a transmission electron microscope (HU12-A; Hitachi, Ltd., Tokyo).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Peyer's patch lymphoid follicle epithelial adherence of a rabbit enteropathogenic Escherichia coli (strain RDEC-1). Role of plasmid-mediated pili in initial adherence.

Inman¹,

Cantey²

1984

J. Clin. Invest.

Self Cite

View full text Add to dashboard Cite

Abstract. Escherichia coli (strain RDEC-1) adheres to M cells of rabbit Peyer's patch lymphoid follicle epithelium. The RDEC-1 strain contains an 85 X 106 D plasmid that codes for pili, which, when purified, adhere to gut absorptive epithelium. This study compared the in vivo lymphoid follicle adherence ofthe RDEC-1 strain with that of a Shigella flexneri (ShD 15) that contained the 85 X 106 D plasmid and expressed the RDEC-l pili, a control E. coli, and a control S. flexneri (ShD 12). The bacteria were given in a dose of 1010 to 0.7-1.1 kg rabbits.The rabbits were sacrificed at 2, 4, 6, and 12 h postinoculation. Peyer's patch tissue was examined by electronmicroscopy and direct fluorescence microscopy. The piliated ShD 15 and RDEC-1 bacteria adhered in large numbers at 2 and 4 h postinoculation, but only the RDEC-1 strain persisted and increased in numbers past that time. Control strains did not adhere. The ShD 15 strain adhered to and was rapidly taken into M cells, precipitating an acute inflammatory reaction within the follicle and adjacent lumen. Initial lymphoid follicle M cell adherence of the ShD 15 strain was associated with the possession of the adherence pilus plasmid. The failure of the ShD 15 strain to survive and colonize the lymph follicle epithelium contrasts with the success ofthe RDEC-1 strain and indicates that the RDEC-1 strain possesses virulence factors in addition to pili.

show abstract

Section: Resultsmentioning

confidence: 96%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Peyer's patch lymphoid follicle epithelial adherence of a rabbit enteropathogenic Escherichia coli (strain RDEC-1). Role of plasmid-mediated pili in initial adherence.

Inman¹,

Cantey²

1984

J. Clin. Invest.

Self Cite

View full text Add to dashboard Cite

show abstract

“…This transcellular route has been documented for tonsillar M-cells by the fluid-phase marker peroxidase (Gebert, 1995) and for M-cells of the intestine by various soluble and solid tracers (Ermak et al, 1995;Amerongen et al, 1992;Owen, 1977). Such substances and likewise complete microorganisms preferentially bind to the surface of M-cells (Sicinski et al, 1990;Inman and Cantey, 1983;Wolf et al, 1981), a process that is regarded as the initial step for their uptake or invasion. Neutra et al (1987) demonstrated that adherence of antigens to the M-cell surface considerably improves the efficiency of the uptake.…”

Section: Discussionmentioning

confidence: 99%

M-cells in the rabbit tonsil exhibit distinctive glycoconjugates in their apical membranes.

Gebert

1996

J Histochem Cytochem.

View full text Add to dashboard Cite

The tonsil crypt epithelium contains membranous (M)-cells that transport antigens from the lumen to underlying lymphoid cells, thereby initiating specific immune responses. Mechanisms mediating the adhesion of antigens to the M-cell surface are important for effective and selective uptake of potential pathogens but are still poorly understood. Therefore, the carbohydrates present on crypt epithelial cells of the rabbit palatine tonsil were studied by lectin histochemistry. Ultrathin LR White sections were incubated with a panel of eight lectins conjugated to colloidal gold or biotin. The glycocalyx of the apical membrane of M-cells was selectively labeled by UEA-I, LTA, HPA, and VVA, whereas that of the remaining squamous epithelial cells preferentially bound RCA-I and PNA. WGA and ConA showed only little binding, with no discernible preference for any of the cell types. Double labeling of UEA-1 together with anti-vimentin antibodies revealed that UEA-I-positive epithelial cells also contained the rabbit M-cell marker vimentin, and vice versa. The results show that a specific composition of glycoconjugates, which differs from that on squamous epithelial cells, is found on M-cells of the rabbit tonsil. The M-cell-specific glycoproteins and glycolipids could be selectively targeted by microorganisms that adhere to M-cells and enter the host along this pathway.

show abstract

“…In addition to interactions with enterocytes, EPEC also interacts with phagocytic M cells and dendritic cells that extend protrusions into the gut lumen where EPEC may encounter infiltrating innate immune cells (Inman and Cantey 1983;Martinez-Argudo et al 2007;Vossenkäm-per et al 2010). These particular immune cell types are modeled by macrophage-like cell lines such as J774 and U937, and using these models, it was found that EPEC resists uptake by professional phagocytes .…”

Section: In Vitro Infection Modelsmentioning

confidence: 99%

In Vitro and In Vivo Model Systems for Studying Enteropathogenic Escherichia coli Infections

Law

Gur-Arie

Rosenshine

et al. 2013

Cold Spring Harbor Perspectives in Medicine

View full text Add to dashboard Cite

Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) belong to a group of bacteria known as attaching and effacing (A/E) pathogens that cause disease by adhering to the lumenal surfaces of their host's intestinal epithelium. EPEC and EHEC are major causes of infectious diarrhea that result in significant childhood morbidity and mortality worldwide. Recent advances in in vitro and in vivo modeling of these pathogens have contributed to our knowledge of how EPEC and EHEC attach to host cells and subvert hostcell signaling pathways to promote infection and cause disease. A more detailed understanding of how these pathogenic microbes infect their hosts and how the host responds to infection could ultimately lead to new therapeutic strategies to help control these significant enteric pathogens.

show abstract

Specific adherence of Escherichia coli (strain RDEC-1) to membranous (M) cells of the Peyer's patch in Escherichia coli diarrhea in the rabbit.

Cited by 181 publications

References 25 publications

Peyer's patch lymphoid follicle epithelial adherence of a rabbit enteropathogenic Escherichia coli (strain RDEC-1). Role of plasmid-mediated pili in initial adherence.

Peyer's patch lymphoid follicle epithelial adherence of a rabbit enteropathogenic Escherichia coli (strain RDEC-1). Role of plasmid-mediated pili in initial adherence.

M-cells in the rabbit tonsil exhibit distinctive glycoconjugates in their apical membranes.

In Vitro and In Vivo Model Systems for Studying Enteropathogenic Escherichia coli Infections

Contact Info

Product

Resources

About