Specific Activation of Insulin-like Growth Factor-1 Receptor by Ginsenoside Rg5 Promotes Angiogenesis and Vasorelaxation

Cho, Young Lai; Hur, Sung Mo; Kim, Ji Yoon; Kim, Jihee; Lee, Dong Hoon; Choe, Jongeon; Won, Moo Ho; Ha, Kwon Soo; Jeoung, Dooil; Han, Sim-Hee; Ryoo, Sungwoo; Lee, Hansoo; Kim, Jung Min; Kwon, Young Guen; Kim, Dong Hyun; Kim, Young Myeong

doi:10.1074/jbc.m114.603142

Cited by 51 publications

(36 citation statements)

References 36 publications

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“…Besides, the VEGF pathway is recognized as the key regulators of the angiogenic process23. Previous studies have also presented vascular smooth muscle contraction signaling pathway24, JAK/STAT1 signaling pathway25, insulin signaling pathway26 and ErbB signaling pathway27 played important roles in angiogenesis. To adapt to the development of loach posterior intestine, such as cell size growing, cell proliferation and migration, the pathways associated with cytoskeleton were found.…”

Section: Discussionmentioning

confidence: 99%

Developmental transcriptome analysis and identification of genes involved in formation of intestinal air-breathing function of Dojo loach, Misgurnus anguillicaudatus

Luo

Cao

et al. 2016

Sci Rep

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Dojo loach, Misgurnus anguillicaudatus is a freshwater fish species of the loach family Cobitidae, using its posterior intestine as an accessory air-breathing organ. Little is known about the molecular regulatory mechanisms in the formation of intestinal air-breathing function of M. anguillicaudatus. Here high-throughput sequencing of mRNAs was performed from six developmental stages of posterior intestine of M. anguillicaudatus: 4-Dph (days post hatch) group, 8-Dph group, 12-Dph group, 20-Dph group, 40-Dph group and Oyd (one-year-old) group. These six libraries were assembled into 81300 unigenes. Totally 40757 unigenes were annotated. Subsequently, 35291 differentially expressed genes (DEGs) were scanned among different developmental stages and clustered into 20 gene expression profiles. Finally, 15 key pathways and 25 key genes were mined, providing potential targets for candidate gene selection involved in formation of intestinal air-breathing function in M. anguillicaudatus. This is the first report of developmental transcriptome of posterior intestine in M. anguillicaudatus, offering a substantial contribution to the sequence resources for this species and providing a deep insight into the formation mechanism of its intestinal air-breathing function. This report demonstrates that M. anguillicaudatus is a good model for studies to identify and characterize the molecular basis of accessory air-breathing organ development in fish.

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Section: Discussionmentioning

confidence: 99%

Developmental transcriptome analysis and identification of genes involved in formation of intestinal air-breathing function of Dojo loach, Misgurnus anguillicaudatus

Luo

Cao

et al. 2016

Sci Rep

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“…The ginsenosides, which consist of triterpene aglycones, are classified into three major categories: panaxidiols, panaxatriols, and oleanolic acid derivatives [2]. Recently, ginsenosides and their derivatives have been reported to show antioxidant [3,4], anti-inflammatory [5][6][7], anti-obesity [6,8], angiogenic [3,9,10], and anticancer activities [11][12][13][14]. The ginsenoside Rh2 (Fig.…”

Section: Introductionmentioning

confidence: 99%

Ginsenoside Rh2 inhibits proliferation and induces apoptosis in human leukemia cells via TNF-α signaling pathway

Huang

Peng

Wang

et al. 2016

ABBS

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Ginsenoside Rh2, a triterpene saponin extracted from Panax ginseng, exhibits pharmacological activity against multiple cancers. However, the anticancer mechanism of ginsenoside Rh2 is unclear. In this study, we found that ginsenoside Rh2 effectively inhibits growth and induces apoptosis of HL-60 cells. Using microarray technology, we found that tumor necrosis factor-α (TNF-α) is clearly up-regulated. Furthermore, anti-TNF-α antibody relieved the Rh2-induced HL-60 cell apoptosis via suppression of caspase-8, caspase-9, and caspase-3 activation. In addition, TNF-α up-regulation was also observed in other Rh2-treated cancer cell lines. These results demonstrate that TNF-α plays a key role in ginsenoside Rh2-induced cell apoptosis.

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“…The phosphorylation sites have been identified in the intracellular domain of IGF-1R, which allow for specific binding and modulation of multiple downstream effectors [27,28]. An earlier study by Wang et al confirmed that low shear stress-induced IGF-1R phosphorylation modulates smooth muscle cell behavior and functions (proliferation and phenotype) via enhancing Akt activation [29].…”

Section: Cellular Physiology and Biochemistry Cellular Physiology Andmentioning

confidence: 66%

Periodic Mechanical Stress Stimulates Cav-1-Dependent IGF-1R Mitogenic Signals in Rat Chondrocytes Through ERK1/2

Tang

Jiang

Sun

et al. 2018

Cell Physiol Biochem

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Background/Aims: The biological effects of periodic mechanical stress on the mitogenesis of chondrocytes have been studied extensively over the past few years. However, the mechanisms underlying the ability of chondrocytes to sense and respond to mechanical stimuli remain to be determined. In the current study, we analyzed the mechanisms by which periodic mechanical stress is translated into biochemical signals and verified the key role of non-integrin mechanosensors including Caveolin-1 (Cav-1), and insulin-like growth factor-1 receptor (IGF-1R) in chondrocyte proliferation. Methods: Two steps were undertaken in the experiment. In the first step, the cells were maintained under static conditions or periodic mechanical stress for 0 h and 1 h prior to Western blot analysis. In the second step, the cells were pretreated with short hairpin RNA (shRNA) targeted to Cav-1 or IGF-1R or control scrambled shRNA. Moreover, they were pretreated with their selective inhibitors methyl β-cyclodextrin (MCD) or Linsitinib (OSI-906). They were maintained under static conditions or periodic mechanical stress for 1 h prior to Western blot analysis, and for 3 days, 8 h per day, prior to direct cell counting and CCK-8 assay, respectively. Results: Periodic mechanical stress significantly induced sustained phosphorylation of Cav-1 at Tyr 14 and IGF-1R at Tyr 1135/1136 . Proliferation was inhibited by pretreatment with Cav-1 inhibitor MCD and by shRNA targeted to Cav-1 in chondrocytes in response to periodic mechanical stress. Meantime, MCD and shRNA targeted to Cav-1 also attenuated IGF-1R, and extracellular signal-regulated kinase (ERK)1/2 activation. In addition, inhibiting IGF-1R activity by Linsitinib and shRNA targeted to

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Specific Activation of Insulin-like Growth Factor-1 Receptor by Ginsenoside Rg5 Promotes Angiogenesis and Vasorelaxation

Cited by 51 publications

References 36 publications

Developmental transcriptome analysis and identification of genes involved in formation of intestinal air-breathing function of Dojo loach, Misgurnus anguillicaudatus

Developmental transcriptome analysis and identification of genes involved in formation of intestinal air-breathing function of Dojo loach, Misgurnus anguillicaudatus

Ginsenoside Rh2 inhibits proliferation and induces apoptosis in human leukemia cells via TNF-α signaling pathway

Periodic Mechanical Stress Stimulates Cav-1-Dependent IGF-1R Mitogenic Signals in Rat Chondrocytes Through ERK1/2

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Specific Activation of Insulin-like Growth Factor-1 Receptor by Ginsenoside Rg5 Promotes Angiogenesis and Vasorelaxation

Cited by 51 publications

References 36 publications

Developmental transcriptome analysis and identification of genes involved in formation of intestinal air-breathing function of Dojo loach, Misgurnus anguillicaudatus

Developmental transcriptome analysis and identification of genes involved in formation of intestinal air-breathing function of Dojo loach, Misgurnus anguillicaudatus

Ginsenoside Rh2 inhibits proliferation and induces apoptosis in human leukemia cells via TNF-&alpha; signaling pathway

Periodic Mechanical Stress Stimulates Cav-1-Dependent IGF-1R Mitogenic Signals in Rat Chondrocytes Through ERK1/2

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Ginsenoside Rh2 inhibits proliferation and induces apoptosis in human leukemia cells via TNF-α signaling pathway