Species variants of tachykinin receptor types

Watling, Keith J.; Guard, Steven; Boyle, S. J.; McKnight, A.T.; Woodruff, G.N.

doi:10.1042/bst0220118

Cited by 11 publications

(2 citation statements)

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“…These peptides and their respective receptors have been implicated in a number of mammalian motor and sensory functions (Helke et al, 1990;Maggi, 1995). There is evidence for three main types of mammalian tachykinin receptors, with species variants and subtypes among each of these three receptor types (Watling et al, 1994).…”

Section: Indexing Terms: Central Body; Fly; Insect; Locust; Corpus Camentioning

confidence: 99%

Immunolocalization of a tachykinin-receptor-like protein in the central nervous system of Locusta migratoria migratorioides and neobellieria bullata

Veelaert¹,

Oonk²,

Eynde³

et al. 1999

J. Comp. Neurol.

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Antisera raised against two distinct peptide regions of the Drosophila neurokinin-like receptor NKD were used to immunolocalize tachykinin-receptor-like proteins in the central nervous system of two insect species: the African migratory locust, Locusta migratoria, and the gray fleshfly, Neobellieria bullata. The resulting immunopositive staining patterns were identical for both antisera. Moreover, a very similar distribution of the immunoreactive material was observed in fleshflies and locusts. Immunoreactivity was found in nerve terminals of the retrocerebral complex, suggesting a presynaptic localization of the receptor in this part of the brain. Cell bodies were stained in the subesophageal ganglion: an anterior group of four larger cells and a posterior group of about 20 cells. These cells have axons projecting into the contralateral nervus corporis allati (NCA) II, bypassing the corpus allatum and projecting through the NCA I into the storage part of the corpus cardiacum. In the glandular part of the corpus cardiacum, the glandular adipokinetic hormone-producing cells did not show any immunopositive staining. In the locust, additional immunopositive staining was observed in internolaterally located neurons of the tritocerebrum and in important integrative parts of the neuropil such as the central body and the mushroom bodies.

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Section: Indexing Terms: Central Body; Fly; Insect; Locust; Corpus Camentioning

confidence: 99%

Immunolocalization of a tachykinin-receptor-like protein in the central nervous system of Locusta migratoria migratorioides and neobellieria bullata

Veelaert¹,

Oonk²,

Eynde³

et al. 1999

J. Comp. Neurol.

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“…These peptide hormones have been implicated in a number of mammalian motor and sensory functions, particularly related to central and peripheral neuron activity and the contraction of smooth muscle 1 . There is evidence for three classes of tachykinin receptors in mammals with variations in amino acid (aa) sequence occurring between species 2 . The classification of receptors is based on interactions with specific natural tachykinins and synthetic agonists which selectively act on only one of the three receptor classes.…”

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confidence: 99%

Cloning of a cDNA from Stable Fly which Encodes a Protein with Homology to a Drosophila Receptor for Tachykinin‐like Peptides

Guerrero

1997

Annals of the New York Academy of Sciences

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Tachykinins are members of a family of peptides which act as neurohormones in mammals. These peptide hormones have been implicated in a number of mammalian motor and sensory functions, particularly related to central and peripheral neuron activity and the contraction of smooth muscle.' There is evidence for three classes of tachykinin receptors in mammals with variations in amino acid (aa) sequence occumng between species.? The classification of receptors is based on interactions with specific natural tachykinins and synthetic agonists which selectively act on only one of the three receptor classes. Tachykinin-like peptides have been purified from invertebrate^,^ and several cDNAs from Drosophila have been cloned which code for proteins with structural and biochemical properties similar to mammalian tachykinin receptor^.^.^ The stable fly, Stomoxys calcitrans, is a major hematophagous pest of livestock causing an estimated $400 million annual loss to the United States livestock industry.6 Current control methodologies include good animal husbandry and insecticide spraying. Ongoing studies seek alternative ways of controlling infestations as concerns increase over the use of insecticides. As part of these studies, we sought to clone the receptor for tachykinin peptides in the stable fly to study the molecular interaction between the receptor and the peptide.A stable fly cDNA library was synthesized in a hgt22 cloning vector using polyA+ RNA from whole adult flies and screened with a Drosophila rnelanogaster DTKR tachykinin receptor DNA probe.4 One 4118 bp clone, designated STKR, was purified and sequenced and found to have an open reading frame which encoded a 678 aa protein with significant homology to the D . melanogaster DTKR open reading frame. The DTKR cDNA is reported to encode a 519 aa receptorlike protein with homology to vertebrae tachykinin receptors and whose mRNA is expressed in the adult insect's central nervous system and specific subsets of neurons in the developing e m b r y~.~ In expression studies, DTKR is activated by vertebrate substance P-like peptides. Homology between the STKR open reading frame and reported tachykinin receptors is highest in the seven putative membrane spanning regions of this receptor family. Comparison of the DTKR and STKR open reading frame sequence spanning transmembrane regions I-VII shows 227 of 283 aligned aa are identical (80%), whereas 266 of 283 (94%) are either identical or conservative substitutions as determined by the pam250S scoring matrix analysis of MacVector Sequence Analysis Software (International Biotechnologies Inc., 310

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Immunolocalization of a tachykinin-receptor-like protein in the central nervous system ofLocusta migratoria migratorioides andneobellieria bullata

Veelaert

Oonk²,

Eynde³

et al. 1999

J. Comp. Neurol.

View full text Add to dashboard Cite

Antisera raised against two distinct peptide regions of the Drosophila neurokinin‐like receptor NKD were used to immunolocalize tachykinin‐receptor‐like proteins in the central nervous system of two insect species: the African migratory locust, Locusta migratoria, and the gray fleshfly, Neobellieria bullata. The resulting immunopositive staining patterns were identical for both antisera. Moreover, a very similar distribution of the immunoreactive material was observed in fleshflies and locusts. Immunoreactivity was found in nerve terminals of the retrocerebral complex, suggesting a presynaptic localization of the receptor in this part of the brain. Cell bodies were stained in the subesophageal ganglion: an anterior group of four larger cells and a posterior group of about 20 cells. These cells have axons projecting into the contralateral nervus corporis allati (NCA) II, bypassing the corpus allatum and projecting through the NCA I into the storage part of the corpus cardiacum. In the glandular part of the corpus cardiacum, the glandular adipokinetic hormone‐producing cells did not show any immunopositive staining. In the locust, additional immunopositive staining was observed in internolaterally located neurons of the tritocerebrum and in important integrative parts of the neuropil such as the central body and the mushroom bodies. J. Comp. Neurol. 407:415–426, 1999. © 1999 Wiley‐Liss, Inc.

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Species variants of tachykinin receptor types

Cited by 11 publications

References 0 publications

Immunolocalization of a tachykinin-receptor-like protein in the central nervous system of Locusta migratoria migratorioides and neobellieria bullata

Immunolocalization of a tachykinin-receptor-like protein in the central nervous system of Locusta migratoria migratorioides and neobellieria bullata

Cloning of a cDNA from Stable Fly which Encodes a Protein with Homology to a Drosophila Receptor for Tachykinin‐like Peptides

Immunolocalization of a tachykinin-receptor-like protein in the central nervous system ofLocusta migratoria migratorioides andneobellieria bullata

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