Species Variability in Platelet Aggregation Response to Different Agonists

Pelagalli, Alessandra; Lombardi, Pietro; d’Angelo, Danila; Morte, Renata Della; Avallone, Luigi; Staiano, Norma

doi:10.1053/jcpa.2002.0570

Cited by 41 publications

(38 citation statements)

References 27 publications

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“…Like sheep, pigs have also been attributed with a coagulation system that is similar to humans, 72,73 although with a tendency towards hypercoagulability. 42 Pelagalli et al compared buffalo, horse, pig, and sheep platelets for their ability to aggregate in response to agonists 74 and to attach to immobilized autologous fibrinogen. 75 Pig platelets were more responsive to ADP than sheep, but sheep platelets demonstrated greater sensitivity to ristocetin, collagen type I, and PAF.…”

Section: Comparative Hematologymentioning

confidence: 99%

Animal models for the assessment of novel vascular conduits

Byrom

Bannon

White

et al. 2010

Journal of Vascular Surgery

149

125

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The development of an ideal small-diameter conduit for use in vascular bypass surgery has yet to be achieved. The ongoing innovation in biomaterial design generates novel conduits that require preclinical assessment in vivo, and a number of animal models have been used for this purpose. This article examines the rationale behind animal models used in the assessment of small-diameter vascular conduits encompassing the commonly used species: baboons, sheep, pigs, dogs, rabbits, and rodents. Studies on the comparative hematology for these species relative to humans are summarized, and the hydrodynamic values for common implant locations are also compared. The large- and small-animal models are then explored, highlighting the characteristics of each that determine their relative utility in the assessment of vascular conduits. Where possible, the performance of expanded polytetrafluoroethylene is given in each animal and in each location to allow direct comparisons between species. New challenges in animal modeling are outlined for the assessment of tissue-engineered graft designs. Finally, recommendations are given for the selection of animal models for the assessment of future vascular conduits.

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Section: Comparative Hematologymentioning

confidence: 99%

Animal models for the assessment of novel vascular conduits

Byrom

Bannon

White

et al. 2010

Journal of Vascular Surgery

149

125

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“…Platelet alpha granules [4,6] contain at least seven GF directly involved in the healing process. Of these proteins, transforming growth factor beta isoform 1 (TGF-β 1 ) is of pivotal importance for its actions on cell proliferation, angiogenesis and extracellular matrix deposition [7].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the effect of calcium gluconate and batroxobin on the release of transforming growth factor beta 1 in canine platelet concentrates

2012

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BackgroundThe clinical use of autologous platelet concentrates (also known as platelet-rich plasma) on the field of regenerative therapy, in the last decade has been the subject of several studies especially in equine medicine and surgery. The objectives of this study was: 1) to describe and compare the cellular population in whole blood, lower fraction (A) and upper fraction (B) of platelet concentrates, 2) to measure and compare the transforming growth factor beta 1 (TGF-β1) concentration in plasma and both platelet concentrates after be activated with calcium gluconate or batroxobin plus calcium gluconate and, 3) to determine correlations between cell counts in platelet concentrates and concentrations of TGF-β1. Blood samples were taken from 16 dogs for complete blood count, plasma collection and platelet concentrates preparation. The platelet concentrates (PC) were arbitrarily divided into two fractions, specifically, PC-A (lower fraction) and PC-B (upper fraction). The Platelet concentrates were analyzed by hemogram. After activated with calcium gluconate or batroxobin plus calcium gluconate, TGF-β1 concentration was determined in supernatants of platelet concentrates and plasma.ResultsThere were differences statistically significant (P < 0.05) for the platelet count and leukocyte count and TGF-β1 concentration between whole blood, plasma and both platelet concentrates. A significant correlation was found between the number of platelets in both platelet concentrates and TGF-β1 concentration. Platelet collection efficiency was 46.34% and 28.16% for PC-A and PC-B, respectively. TGF-β1 concentration efficiency for PC activated with calcium gluconate was 47.75% and 31.77%, for PC-A and PC-B, respectively. PC activated with batroxobin plus CG showed 46.87% and 32.24% for PC-A and PC-B, respectively.ConclusionsThe methodology used in this study allows the concentration of a number of platelets and TGF-β1 that might be acceptable for a biological effect for clinical or experimental use as a regenerative therapy in dogs.

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“…The efficiency with which platelets adhere and aggregate at the sites of vessel wall injury is dependent on the synergistic action of various adhesive and soluble agonist receptors, with the contribution of each of the individual receptors dependent on the prevailing blood flow conditions (Jackson et al, 2003). The activation of platelets is regulated and modulated by numerous relatively well-characterized factors, including adenosine diphosphate (ADP) (Pelagalli et al, 2002). Moreover, since it is known that exercise has variable effects on equine blood parameters (Piccione et al, 2008), it also causes modifications of platelet function (Sakita et al, 1997;El-Sayed et al, 2000), although the exact mechanisms and the regulatory pathways involved in the effects of exercise on platelet function are not completely understood.…”

Section: Introductionmentioning

confidence: 99%

Modifications of platelet aggregation during treadmill section and obstacle course in athletic horse

Piccione¹,

Assenza²,

Casella³

et al. 2010

Acta vet. (Beogr.)

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Eight clinically healthy Sella Italiana horses were used in order to assess the variations of platelet aggregation in response to different workload. Blood samples were collected from each horse at rest, immediately after exercise and 30 min after the end of the exercise. By means of an aggregometer platelet aggregation was quantitated as maximum degree of platelet aggregation and initial velocity platelet aggregation. Two-way repeated measures analysis of variance (ANOVA) was applied to determine significant differences (p<0.05) due to the intensity of exercise and different experimental condition. Our results showed a similar trend of platelet aggregation between treadmill and obstacle course, but statistically significant differences emerged on studied parameters in relation to different workload. These results showed an inhibition of platelet response that probably represents a protective endothelial mechanism through the production of nitric oxide during different workloads in athletic horses

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Species Variability in Platelet Aggregation Response to Different Agonists

Cited by 41 publications

References 27 publications

Animal models for the assessment of novel vascular conduits

Animal models for the assessment of novel vascular conduits

Comparison of the effect of calcium gluconate and batroxobin on the release of transforming growth factor beta 1 in canine platelet concentrates

Modifications of platelet aggregation during treadmill section and obstacle course in athletic horse

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